PMID- 28004965
OWN - NLM
STAT- MEDLINE
DCOM- 20171116
LR  - 20171128
IS  - 1130-0108 (Print)
IS  - 1130-0108 (Linking)
VI  - 109
IP  - 3
DP  - 2017 Mar
TI  - Restoration of density of interstitial cells of Cajal in the jejunum of diabetic 
      rats after quercetin supplementation.
PG  - 190-195
LID - 10.17235/reed.2016.4338/2016 [doi]
AB  - BACKGROUND: Interstitial cells of Cajal (ICC) are required for normal motility in 
      the gastrointestinal tract. Depletion of ICC has been associated with diabetic 
      gastroenteropathy. PURPOSE: To determine the effect of quercertin supplementation 
      on anoctamin-1 (Ano1) immunoreactive ICC in the myenteric region (ICC-MY) and 
      deep muscular plexus (ICC-DMP) in the jejunum of diabetic rats. METHODS: 
      Thirty-two 90-day-old male Wistar rats were distributed into the following 
      groups: normoglycemic (C), normoglycemic supplemented with quercetin (CQ; 40 mg 
      daily), diabetic (D), and diabetic supplemented with quercetin (DQ; 40 mg daily). 
      Diabetes was induced by streptozotocin injection. After 120 days, preparations of 
      the jejunal muscular and submucosal layers were immunostained for Ano1 to 
      visualize ICC. Evaluation of the immunofluorescence intensity as well as density 
      of ICC was performed. RESULTS: The density of ICC-MY was 46% lower in group D 
      compared to group C (p < 0.01); ICC-DMP were reduced by 37% (p > 0.05). After 
      quercertin treatment, the densities of ICC-MY were significantly higher in the DQ 
      group compared to group D (ICC-MY: 58%, p < 0.05). Supplementation with quercetin 
      in normoglycemic animals (CQ) compared with group C did not significantly change 
      the ICC density (p > 0.05). CONCLUSIONS: In STZ-treated diabetic rats, diabetes 
      promoted a reduction in the density of jejunal ICC-MY with no significant effect 
      on ICC-DMP. Supplementation with quercetin (DQ) appeared to protect ICC-MY from 
      depletion in diabetes possibly due to its antioxidant action.
FAU - Vieira Frez, Flavia Cristina
AU  - Vieira Frez FC
AD  - Morphological Science, State University of Maringa, Brazil.
FAU - Martins Colombo Perles, Juliana Vanessa
AU  - Martins Colombo Perles JV
AD  - Department of Morphological Sciences, State University of Maringa.
FAU - Robert Linden, David
AU  - Robert Linden D
AD  - Mayo Clinic.
FAU - Gibbons, Simon John
AU  - Gibbons SJ
AD  - Physiology and Biomedical Engineering and Enteric, Mayo Clinic, USA.
FAU - Amilcar Martins, Heber
AU  - Amilcar Martins H
AD  - Universidade Estadual de Maringa.
FAU - Almeida Brito Romualdo, Debora
AU  - Almeida Brito Romualdo D
AD  - Morphological Science, Universidade Estadual de Maringa, Brazil.
FAU - de Souza, Sara Raquel
AU  - de Souza SR
AD  - Department of Morphological Sciences, State University of Maringa.
FAU - Daion Piovezana Bossolani, Gleison
AU  - Daion Piovezana Bossolani G
AD  - Universidade Estadual de Maringa.
FAU - Zanoni, Jacqueline Nelisis
AU  - Zanoni JN
AD  - Morphological Sciences, State University of Maringa, Brazil.
LA  - eng
PT  - Journal Article
PL  - Spain
TA  - Rev Esp Enferm Dig
JT  - Revista espanola de enfermedades digestivas
JID - 9007566
RN  - 0 (ANO1 protein, rat)
RN  - 0 (Anoctamin-1)
RN  - 0 (Antioxidants)
RN  - 9IKM0I5T1E (Quercetin)
SB  - IM
MH  - Animals
MH  - Anoctamin-1/metabolism
MH  - Antioxidants/*therapeutic use
MH  - Diabetes Mellitus, Experimental/*drug therapy/pathology
MH  - *Dietary Supplements
MH  - Interstitial Cells of Cajal/*drug effects/pathology
MH  - Jejunum/drug effects/*pathology
MH  - Male
MH  - Quercetin/*therapeutic use
MH  - Rats
MH  - Rats, Wistar
EDAT- 2016/12/23 06:00
MHDA- 2017/11/29 06:00
CRDT- 2016/12/23 06:00
PHST- 2016/12/23 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/12/23 06:00 [entrez]
AID - 10.17235/reed.2016.4338/2016 [doi]
PST - ppublish
SO  - Rev Esp Enferm Dig. 2017 Mar;109(3):190-195. doi: 10.17235/reed.2016.4338/2016.