PMID- 28007956
OWN - NLM
STAT- MEDLINE
DCOM- 20170830
LR  - 20180510
IS  - 1460-2180 (Electronic)
IS  - 0143-3334 (Linking)
VI  - 38
IP  - 2
DP  - 2017 Feb 1
TI  - Upregulation of RPA2 promotes NF-kappaB activation in breast cancer by relieving the 
      antagonistic function of menin on NF-kappaB-regulated transcription.
PG  - 196-206
LID - 10.1093/carcin/bgw123 [doi]
AB  - RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is 
      overexpressed in various cancers. In this study, we showed a significant RPA2 
      upregulation in breast cancer tissues and cell lines. Ectopic expression of RPA2 
      in MCF7 and MDA-MB-231 cells promoted cell proliferation, adhesion, migration and 
      invasion, and induced epithelial-mesenchymal transition (EMT) of MCF7 cells. 
      Ablation of RPA2 in MDA-MB-231 cells induced apoptosis and suppressed colony 
      formation, EMT and invasion. Binding assays indicated that menin, the multiple 
      endocrine neoplasia type 1 (MEN1) tumor suppressor gene product, interacted with 
      RPA2. Ectopic expression of RPA2 inhibited the formation of the menin-NK-kappaB p65 
      complex and repressed the inhibitory effect of menin on expression of 
      NF-kappaB-regulated genes that contribute to tumor progression. Conversely, knockdown 
      of RPA2 promoted formation of the menin-p65 complex and repressed the expression 
      of NF-kappaB-mediated genes. RPA2 expression was induced via an E2F1-dependent 
      mechanism in MCF7 and MDA-MB-231 cells treated with NF-kappaB activators, TNF-alpha 
      or lipopolysaccharide (LPS). These results suggested that RPA2-dependent 
      tumorigenicity was mediated via enhancement of NF-kappaB activity by relieving the 
      antagonistic function of menin on NF-kappaB-regulated transcription in breast cancer 
      cells.
CI  - (c) The Author 2016. Published by Oxford University Press. All rights reserved. For 
      Permissions, please email: journals.permissions@oup.com.
FAU - Chen, Chao-Chung
AU  - Chen CC
AD  - Department of Biotechnology, College of Medicine and Nursing, Hung Kuang 
      University, Taichung 43302, Taiwan.
FAU - Juan, Chi-Wen
AU  - Juan CW
AD  - Department of Emergency, Kuang Tien General Hospital, Taichung 43302, Taiwan.
AD  - Department of Nursing, College of Medicine and Nursing, Hung Kuang University, 
      Taichung 43302, Taiwan.
FAU - Chen, Kuan-Yu
AU  - Chen KY
AD  - Hepatobiliary Division, Department of Internal Medicine, Kaohsiung Medical 
      University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
FAU - Chang, Yi-Chien
AU  - Chang YC
AD  - Department of Surgery, National Cheng Kung University Medical College and 
      Hospital, Tainan 70101, Taiwan and.
FAU - Lee, Janq-Chang
AU  - Lee JC
AD  - Department of Surgery, National Cheng Kung University Medical College and 
      Hospital, Tainan 70101, Taiwan and.
FAU - Chang, Ming-Chung
AU  - Chang MC
AD  - Department of Nutrition, College of Medicine and Nursing, Hung Kuang University, 
      Taichung 43302, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (MEN1 protein, human)
RN  - 0 (NF-kappa B)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Replication Protein A)
RN  - EC 2.7.7.7 (RPA2 protein, human)
SB  - IM
MH  - Apoptosis/genetics
MH  - Breast Neoplasms/*genetics/pathology
MH  - Carcinogenesis/genetics
MH  - Cell Adhesion/genetics
MH  - Cell Movement/genetics
MH  - Cell Proliferation/*genetics
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - MCF-7 Cells
MH  - NF-kappa B/genetics
MH  - Neoplasm Invasiveness/genetics/pathology
MH  - Proto-Oncogene Proteins/*genetics/metabolism
MH  - Replication Protein A/*biosynthesis/genetics
MH  - Signal Transduction/genetics
EDAT- 2016/12/23 06:00
MHDA- 2017/08/31 06:00
CRDT- 2016/12/24 06:00
PHST- 2016/04/20 00:00 [received]
PHST- 2016/11/16 00:00 [accepted]
PHST- 2016/12/23 06:00 [pubmed]
PHST- 2017/08/31 06:00 [medline]
PHST- 2016/12/24 06:00 [entrez]
AID - bgw123 [pii]
AID - 10.1093/carcin/bgw123 [doi]
PST - ppublish
SO  - Carcinogenesis. 2017 Feb 1;38(2):196-206. doi: 10.1093/carcin/bgw123.