PMID- 28008587
OWN - NLM
STAT- MEDLINE
DCOM- 20170317
LR  - 20181202
IS  - 1439-3646 (Electronic)
IS  - 0947-7349 (Linking)
VI  - 125
IP  - 2
DP  - 2017 Feb
TI  - Neutral Ceramidase Secreted Via Exosome Protects Against Palmitate-Induced 
      Apoptosis in INS-1 Cells.
PG  - 130-135
LID - 10.1055/s-0042-116314 [doi]
AB  - Aims: To investigate the effects of neutral ceramidase (NCDase) packaged in 
      exosomes that are secreted from beta-cells on free fatty acid (FFA)-induced beta-cells 
      apoptosis and its role in regulation of sphingolipid-mediated signaling pathway. 
      Methods: HPLC and Western blotting were performed to determine NCDase activity 
      and expression. Annexin V-fluorescein-isothiocyanate/propidium iodide flow 
      cytometry was used to assess apoptosis. Electrospray ionization tandem mass 
      spectrometry was used for ceramide (Cer), sphingosine-1-phosphate (S1P), and 
      sphingosine (SPH) determination. Results: INS-1 cells over-expressed NCDase 
      secreted active NCDase via exosomes. Exosomes isolated from the cultured medium 
      of INS-1 cells that oxpressed NCDase could ameliorate palmitate-induced 
      apoptosis. Furthermore, the results showed that exosome-derived NCDase treatment 
      reduced intracellular Cer/S1P ratio. Conclusions: beta-cell secreted active NCDase 
      via exosome, the exosome-packaged-NCDase protected beta-cells from FFA-induced 
      apoptosis through regulating sphingolipid metabolites and it might be a potential 
      treatment for beta-cell lipotoxicity and type 2 diabetes.
CI  - (c) Georg Thieme Verlag KG Stuttgart . New York.
FAU - Tang, S
AU  - Tang S
AD  - Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
FAU - Luo, F
AU  - Luo F
AD  - Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
FAU - Feng, Y M
AU  - Feng YM
AD  - Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
FAU - Wei, X
AU  - Wei X
AD  - Endocrine and Diabetes Center, Jiangsu Province Hospital on Integration of 
      Chinese and Western Medicine, Nanjing University of Traditional Chinese Medicine, 
      Branch of China Academy of Chinese Medical Science, Nanjing, China.
FAU - Miao, H
AU  - Miao H
AD  - Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
FAU - Lu, Y B
AU  - Lu YB
AD  - Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
FAU - Tang, Y
AU  - Tang Y
AD  - Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
FAU - Ding, D F
AU  - Ding DF
AD  - Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
FAU - Jin, J F
AU  - Jin JF
AD  - China-USA Lipids in Health and Disease Research Center, Guilin Medical 
      University, Guilin, China.
FAU - Zhu, Q
AU  - Zhu Q
AD  - Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
LA  - eng
PT  - Journal Article
DEP - 20161222
PL  - Germany
TA  - Exp Clin Endocrinol Diabetes
JT  - Experimental and clinical endocrinology & diabetes : official journal, German 
      Society of Endocrinology [and] German Diabetes Association
JID - 9505926
RN  - 0 (Sphingolipids)
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - EC 3.5.1.23 (Neutral Ceramidase)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cell Line
MH  - Diabetes Mellitus, Type 2/metabolism/pathology/therapy
MH  - Exosomes/*metabolism
MH  - Insulin-Secreting Cells/*metabolism/pathology
MH  - Neutral Ceramidase/*metabolism
MH  - Palmitic Acid/*toxicity
MH  - Rats
MH  - Sphingolipids/metabolism
EDAT- 2016/12/23 06:00
MHDA- 2017/03/18 06:00
CRDT- 2016/12/24 06:00
PHST- 2016/12/23 06:00 [pubmed]
PHST- 2017/03/18 06:00 [medline]
PHST- 2016/12/24 06:00 [entrez]
AID - 10.1055/s-0042-116314 [doi]
PST - ppublish
SO  - Exp Clin Endocrinol Diabetes. 2017 Feb;125(2):130-135. doi: 
      10.1055/s-0042-116314. Epub 2016 Dec 22.