PMID- 28010925 OWN - NLM STAT- MEDLINE DCOM- 20170523 LR - 20210920 IS - 1879-3592 (Electronic) IS - 1383-5718 (Linking) VI - 813 DP - 2017 Jan TI - Effects of chronic restraint-induced stress on radiation-induced chromosomal aberrations in mouse splenocytes. PG - 18-26 LID - S1383-5718(16)30067-5 [pii] LID - 10.1016/j.mrgentox.2016.11.005 [doi] AB - Both ionizing radiation (IR) and psychological stress (PS) cause detrimental effects on humans. A recent study showed that chronic restraint-induced PS (CRIPS) diminished the functions of Trp53 and enhanced radiocarcinogenesis in Trp53-heterozygous (Trp53(+/-)) mice. These findings had a marked impact on the academic field as well as the general public, particularly among residents living in areas radioactively contaminated by nuclear accidents. In an attempt to elucidate the modifying effects of CRIPS on radiation-induced health consequences in Trp53 wild-type (Trp53(+/+)) animals, investigations involving multidisciplinary analyses were performed. We herein demonstrated that CRIPS induced changes in the frequency of IR-induced chromosomal aberrations (CAs) in splenocytes. Five-week-old male Trp53(+/+) C57BL/6J mice were restrained for 6h per day for 28 consecutive days, and total body irradiation (TBI) at a dose of 4Gy was performed on the 8th day. Metaphase chromosome spreads prepared from splenocytes at the end of the 28-day restraint regimen were painted with fluorescence in situ hybridization (FISH) probes for chromosomes 1, 2, and 3. The results obtained showed that CRIPS alone did not induce CAs, while TBI caused significant increases in CAs, mostly translocations. Translocations appeared at a lower frequency in mice exposed to TBI plus CRIPS than in those exposed to TBI alone. No significant differences were observed in the frequencies of the other types of CAs (insertions, dicentrics, and fragments) visualized with FISH between these experimental groups (TBI+CRIPS vs. TBI). These results suggest that CRIPS does not appear to synergize with the clastogenicity of IR. CI - Copyright (c) 2016 Elsevier B.V. All rights reserved. FAU - Katsube, Takanori AU - Katsube T AD - National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: katsube.takanori@qst.go.jp. FAU - Wang, Bing AU - Wang B AD - National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: wang.bing@qst.go.jp. FAU - Tanaka, Kaoru AU - Tanaka K AD - National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: tanaka.kaoru@qst.go.jp. FAU - Ninomiya, Yasuharu AU - Ninomiya Y AD - National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: ninomiya.yasuharu@qst.go.jp. FAU - Vares, Guillaume AU - Vares G AD - Advanced Medical Instrumentation Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa, 904-0495, Japan. Electronic address: guillaume.vares@oist.jp. FAU - Kawagoshi, Taiki AU - Kawagoshi T AD - National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: kawagoshi.taiki@qst.go.jp. FAU - Shiomi, Naoko AU - Shiomi N AD - National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: shiomi.naoko@qst.go.jp. FAU - Kubota, Yoshihisa AU - Kubota Y AD - National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: kubota.yoshihisa@qst.go.jp. FAU - Liu, Qiang AU - Liu Q AD - Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, PR China. Electronic address: liuqiang@irm-cams.ac.cn. FAU - Morita, Akinori AU - Morita A AD - Department of Biomedical Science and Technology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8503, Japan. Electronic address: morita@tokushima-u.ac.jp. FAU - Nakajima, Tetsuo AU - Nakajima T AD - National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: nakajima.tetsuo@qst.go.jp. FAU - Nenoi, Mitsuru AU - Nenoi M AD - National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: nenoi.mitsuru@qst.go.jp. LA - eng PT - Journal Article DEP - 20161122 PL - Netherlands TA - Mutat Res Genet Toxicol Environ Mutagen JT - Mutation research. Genetic toxicology and environmental mutagenesis JID - 101632149 RN - 0 (Tumor Suppressor Protein p53) SB - IM MH - Animals MH - *Chromosome Aberrations MH - *Immobilization MH - Male MH - Mice MH - Mice, Inbred C57BL MH - Spleen/*radiation effects MH - *Stress, Physiological MH - Tumor Suppressor Protein p53/genetics MH - Whole-Body Irradiation OTO - NOTNLM OT - Chronic restraint-induced psychological stress OT - Fluorescence in situ hybridization (FISH) OT - Mouse restraint model OT - Total body irradiation EDAT- 2016/12/25 06:00 MHDA- 2017/05/24 06:00 CRDT- 2016/12/25 06:00 PHST- 2016/03/04 00:00 [received] PHST- 2016/10/25 00:00 [revised] PHST- 2016/11/21 00:00 [accepted] PHST- 2016/12/25 06:00 [entrez] PHST- 2016/12/25 06:00 [pubmed] PHST- 2017/05/24 06:00 [medline] AID - S1383-5718(16)30067-5 [pii] AID - 10.1016/j.mrgentox.2016.11.005 [doi] PST - ppublish SO - Mutat Res Genet Toxicol Environ Mutagen. 2017 Jan;813:18-26. doi: 10.1016/j.mrgentox.2016.11.005. Epub 2016 Nov 22.