PMID- 28011154
OWN - NLM
STAT- MEDLINE
DCOM- 20170726
LR  - 20181202
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 40
DP  - 2017 Apr
TI  - Persistent organic pollutants alter DNA methylation during human adipocyte 
      differentiation.
PG  - 79-87
LID - S0887-2333(16)30267-3 [pii]
LID - 10.1016/j.tiv.2016.12.011 [doi]
AB  - Ubiquitous persistent organic pollutants (POPs) can accumulate in humans where 
      they might influence differentiation of adipocytes. The aim of this study was to 
      investigate whether DNA methylation is one of the underlying mechanisms by which 
      POPs affect adipocyte differentiation, and to what extent DNA methylation can be 
      related to gene transcription. Adipocyte differentiation was induced in two human 
      cell models with continuous exposure to different POPs throughout 
      differentiation. From the seven tested POPs, perfluorooctanesulfonic acid (PFOS) 
      and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) decreased lipid accumulation, 
      while tributyltin (TBT) increased lipid accumulation. In human mesenchymal stem 
      cells (hMSCs), TCDD and TBT induced opposite gene expression profiles, whereas 
      after PFOS exposure gene expression remained relatively stable. Genome-wide DNA 
      methylation analysis showed that all three POPs affected DNA methylation patterns 
      in adipogenic and other genes, possibly related to the phenotypic outcome, but 
      without concomitant gene expression changes. Differential methylation was 
      predominantly detected in intergenic regions, where the biological relevance of 
      alterations in DNA methylation is unclear. This study demonstrates that POPs, at 
      environmentally relevant levels, are able to induce differential DNA methylation 
      in human differentiating adipocytes.
CI  - Copyright (c) 2017 Wageningen University. Published by Elsevier Ltd.. All rights 
      reserved.
FAU - van den Dungen, Myrthe W
AU  - van den Dungen MW
AD  - Division of Human Nutrition, Wageningen University, The Netherlands; Marine 
      Animal Ecology group, Wageningen University, The Netherlands. Electronic address: 
      Myrthe.vandenDungen@wur.nl.
FAU - Murk, Albertinka J
AU  - Murk AJ
AD  - Marine Animal Ecology group, Wageningen University, The Netherlands. Electronic 
      address: Tinka.Murk@wur.nl.
FAU - Kok, Dieuwertje E
AU  - Kok DE
AD  - Division of Human Nutrition, Wageningen University, The Netherlands. Electronic 
      address: Dieuwertje.Kok@wur.nl.
FAU - Steegenga, Wilma T
AU  - Steegenga WT
AD  - Division of Human Nutrition, Wageningen University, The Netherlands. Electronic 
      address: Wilma.Steegenga@wur.nl.
LA  - eng
PT  - Journal Article
DEP - 20161220
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Alkanesulfonic Acids)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Fluorocarbons)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (Trialkyltin Compounds)
RN  - 4XDX163P3D (tributyltin)
RN  - 9H2MAI21CL (perfluorooctane sulfonic acid)
SB  - IM
MH  - Adipocytes/cytology/*drug effects/metabolism
MH  - Adipogenesis/genetics
MH  - Alkanesulfonic Acids/toxicity
MH  - Cell Differentiation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - DNA Methylation/*drug effects
MH  - Environmental Pollutants/*toxicity
MH  - Fluorocarbons/toxicity
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*drug effects/metabolism
MH  - Polychlorinated Dibenzodioxins/toxicity
MH  - Transcriptome/drug effects
MH  - Trialkyltin Compounds/toxicity
OTO - NOTNLM
OT  - Adipocytes
OT  - DNA methylation
OT  - Gene expression
OT  - Human mesenchymal stem cells
OT  - Infinium 450K BeadChip
OT  - Persistent organic pollutants (POPs)
EDAT- 2016/12/25 06:00
MHDA- 2017/07/27 06:00
CRDT- 2016/12/25 06:00
PHST- 2016/07/02 00:00 [received]
PHST- 2016/10/17 00:00 [revised]
PHST- 2016/12/18 00:00 [accepted]
PHST- 2016/12/25 06:00 [pubmed]
PHST- 2017/07/27 06:00 [medline]
PHST- 2016/12/25 06:00 [entrez]
AID - S0887-2333(16)30267-3 [pii]
AID - 10.1016/j.tiv.2016.12.011 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2017 Apr;40:79-87. doi: 10.1016/j.tiv.2016.12.011. Epub 2016 
      Dec 20.