PMID- 28024794
OWN - NLM
STAT- MEDLINE
DCOM- 20170531
LR  - 20211204
IS  - 2352-3840 (Electronic)
IS  - 1499-2671 (Linking)
VI  - 41
IP  - 2
DP  - 2017 Apr
TI  - Does Resveratrol Improve Insulin Signalling in HepG2 Cells?
PG  - 211-216
LID - S1499-2671(15)30080-0 [pii]
LID - 10.1016/j.jcjd.2016.09.015 [doi]
AB  - OBJECTIVES: Diabetes mellitus is a common metabolic disorder with high global 
      prevalence. It is characterized by a decrease in insulin secretion or a decrease 
      in insulin sensitivity or both. The aim of the present study was to investigate 
      the effects of resveratrol treatment on the expression of the genes involved in 
      insulin signalling cascade, such as Forkhead box protein O1 (FoxO1), 
      3-phosphoinositide-dependent protein kinase 1 (PDPK1) and mammalian target of 
      rapamycin (mTOR). METHODS: HepG2 cells were cultured in serum-free medium with 
      high concentrations of glucose and insulin and then were treated with resveratrol 
      (5, 10 and 20 microM) for 24 and 48 hours. Complementary deoxyribonucleic acids 
      (cDNAs) were synthesized followed by RNA extraction. Real-time quantitative 
      reverse transcription polymerase chain reaction was used to analyze the 
      expression of FoxO1, PDPK1 and mTOR. RESULTS: Resveratrol increased the 
      expression of PDPK1, mTOR and FoxO1. No significant difference was seen among 
      differing dosages of resveratrol, but treatments for 48 hours exerted the 
      greatest effectiveness. CONCLUSIONS: Our results were consistent with other 
      studies showing the beneficial effects of resveratrol on diabetes. However, 
      considering the effects of resveratrol in increasing FoxO1 and gluconeogenic gene 
      expression, long-term usage of resveratrol should be investigated in greater 
      depth in future studies.
CI  - Copyright (c) 2016 Canadian Diabetes Association. Published by Elsevier Inc. All 
      rights reserved.
FAU - Norouzzadeh, Marjan
AU  - Norouzzadeh M
AD  - Department of Cellular and Molecular Nutrition, School of Nutritional Sciences 
      and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
FAU - Amiri, Fatemehsadat
AU  - Amiri F
AD  - Department of Nutrition, School of Public Health, Iran University of Medical 
      Sciences, Tehran, Iran.
FAU - Saboor-Yaraghi, Ali Akbar
AU  - Saboor-Yaraghi AA
AD  - Department of Cellular and Molecular Nutrition, School of Nutritional Sciences 
      and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
FAU - Shemirani, Farnoosh
AU  - Shemirani F
AD  - Department of Cellular and Molecular Nutrition, School of Nutritional Sciences 
      and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
FAU - Kalikias, Yas
AU  - Kalikias Y
AD  - Department of Cellular and Molecular Nutrition, School of Nutritional Sciences 
      and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
FAU - Sharifi, Loghman
AU  - Sharifi L
AD  - Department of Cellular and Molecular Nutrition, School of Nutritional Sciences 
      and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
FAU - Seyyedsalehi, Monireh Sadat
AU  - Seyyedsalehi MS
AD  - Department of Cellular and Molecular Nutrition, School of Nutritional Sciences 
      and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
FAU - Mahmoudi, Maryam
AU  - Mahmoudi M
AD  - Department of Cellular and Molecular Nutrition, School of Nutritional Sciences 
      and Dietetic, Tehran University of Medical Sciences, Tehran, Iran. Electronic 
      address: mahmoodi_maryam@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20161223
PL  - Canada
TA  - Can J Diabetes
JT  - Canadian journal of diabetes
JID - 101148810
RN  - 0 (FOXO1 protein, human)
RN  - 0 (Forkhead Box Protein O1)
RN  - 0 (Insulin)
RN  - 0 (Stilbenes)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (3-Phosphoinositide-Dependent Protein Kinases)
RN  - EC 2.7.11.1 (PDPK1 protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - 3-Phosphoinositide-Dependent Protein Kinases/genetics/metabolism
MH  - Forkhead Box Protein O1/genetics/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Hep G2 Cells
MH  - Humans
MH  - Insulin/*metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Resveratrol
MH  - Signal Transduction/drug effects
MH  - Stilbenes/*pharmacology
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
OTO - NOTNLM
OT  - FoxO1
OT  - HepG2
OT  - PDPK1
OT  - insulin resistance
OT  - insulinoresistance
OT  - mTOR
OT  - resveratrol
OT  - resveratrol
EDAT- 2016/12/28 06:00
MHDA- 2017/06/01 06:00
CRDT- 2016/12/28 06:00
PHST- 2015/12/24 00:00 [received]
PHST- 2016/09/05 00:00 [revised]
PHST- 2016/09/28 00:00 [accepted]
PHST- 2016/12/28 06:00 [pubmed]
PHST- 2017/06/01 06:00 [medline]
PHST- 2016/12/28 06:00 [entrez]
AID - S1499-2671(15)30080-0 [pii]
AID - 10.1016/j.jcjd.2016.09.015 [doi]
PST - ppublish
SO  - Can J Diabetes. 2017 Apr;41(2):211-216. doi: 10.1016/j.jcjd.2016.09.015. Epub 
      2016 Dec 23.