PMID- 28025169
OWN - NLM
STAT- MEDLINE
DCOM- 20170201
LR  - 20181202
IS  - 1872-7786 (Electronic)
IS  - 0009-2797 (Linking)
VI  - 263
DP  - 2017 Feb 1
TI  - Evaluation of in vitro toxicity of polymeric micelles to human endothelial cells 
      under different conditions.
PG  - 46-54
LID - S0009-2797(16)30435-5 [pii]
LID - 10.1016/j.cbi.2016.12.014 [doi]
AB  - Polymeric micelles have been extensively studied in the area of antitumor 
      therapy, and more recently explored as nanocarriers for atherosclerosis. These 
      applications of polymeric micelles in biomedicine will increase their contact 
      with human blood vessels. However, few studies have considered the interactions 
      between polymeric micelles and endothelial cells, especially in a complex system. 
      This study used human umbilical vein endothelial cells (HUVECs) as an in vitro 
      model for endothelial cells to investigate the toxic effects of 
      methoxy-poly(ethylene glycol)-poly(d,l-lactide) (MPEG-PLA) based micelles. In 
      addition, an endoplasmic reticulum stress inducer thapsigargin (TG), and a 
      pro-atherogenic stimulus palmitate (PA), were used to co-expose HUVECs to further 
      mimic the responses of diseased endothelial cells to micelle exposure. Overall, 
      up to 200 mug/mL micelles did not significantly induce cytotoxicity, reactive 
      oxygen species (ROS), release of inflammatory mediators in terms of interleukin 6 
      (IL-6), IL-8 and soluble vascular cell adhesion molecule 1 (sVCAM-1), or adhesion 
      of THP-1 monocytes to HUVECs. TG and PA significantly induced cytotoxicity and 
      THP-1 adhesion as well as modestly promoted the release of IL-6, but did not 
      affect ROS or release of sVCAM-1 and IL-8. Co-exposure of micelles did not 
      significantly enhance the effects of TG and PA to HUVECs, and ANOVA analysis 
      indicated no interaction between concentrations of micelles and the presence of 
      TG/PA. Taken together, these data indicated that micelles are not toxic to HUVECs 
      under different conditions in vitro.
CI  - Copyright (c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Liu, Fang
AU  - Liu F
AD  - Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry of 
      Education, Laboratory of Biochemistry, College of Chemistry, Xiangtan University, 
      Xiangtan 411105, PR China.
FAU - Huang, Haikang
AU  - Huang H
AD  - Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry of 
      Education, Key Laboratory of Advanced Functional Polymeric Materials of College 
      of Hunan Province and Key Laboratory of Polymeric Materials & Application 
      Technology of Hunan Province, College of Chemistry, Xiangtan University, Xiangtan 
      411105, China.
FAU - Gong, Yu
AU  - Gong Y
AD  - Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry of 
      Education, Laboratory of Biochemistry, College of Chemistry, Xiangtan University, 
      Xiangtan 411105, PR China.
FAU - Li, Juan
AU  - Li J
AD  - Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry of 
      Education, Laboratory of Biochemistry, College of Chemistry, Xiangtan University, 
      Xiangtan 411105, PR China.
FAU - Zhang, Xuefei
AU  - Zhang X
AD  - Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry of 
      Education, Key Laboratory of Advanced Functional Polymeric Materials of College 
      of Hunan Province and Key Laboratory of Polymeric Materials & Application 
      Technology of Hunan Province, College of Chemistry, Xiangtan University, Xiangtan 
      411105, China. Electronic address: zxf7515@163.com.
FAU - Cao, Yi
AU  - Cao Y
AD  - Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry of 
      Education, Laboratory of Biochemistry, College of Chemistry, Xiangtan University, 
      Xiangtan 411105, PR China. Electronic address: caoyi39@xtu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20161223
PL  - Ireland
TA  - Chem Biol Interact
JT  - Chemico-biological interactions
JID - 0227276
RN  - 0 (Drug Carriers)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Micelles)
RN  - 0 (Palmitates)
RN  - 0 (Polyesters)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 0 (methoxy poly(ethylene glycol)-poly(lactide))
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 67526-95-8 (Thapsigargin)
SB  - IM
MH  - Cell Adhesion/drug effects
MH  - Cell Line
MH  - Drug Carriers/chemistry/toxicity
MH  - Endoplasmic Reticulum Stress/*drug effects
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/metabolism
MH  - *Micelles
MH  - Monocytes/cytology/immunology
MH  - Palmitates/toxicity
MH  - Polyesters/chemistry/*toxicity
MH  - Polyethylene Glycols/chemistry/*toxicity
MH  - Reactive Oxygen Species/metabolism
MH  - Thapsigargin/toxicity
MH  - Vascular Cell Adhesion Molecule-1/metabolism
OTO - NOTNLM
OT  - Human umbilical vein endothelial cells (HUVECs)
OT  - Methoxy-poly(ethylene glycol)-poly(D,L-lactide) (MPEG-PLA)
OT  - Micelles
OT  - Palmitate (PA)
OT  - Thapsigargin (TG)
EDAT- 2016/12/28 06:00
MHDA- 2017/02/02 06:00
CRDT- 2016/12/28 06:00
PHST- 2016/10/06 00:00 [received]
PHST- 2016/12/16 00:00 [revised]
PHST- 2016/12/22 00:00 [accepted]
PHST- 2016/12/28 06:00 [pubmed]
PHST- 2017/02/02 06:00 [medline]
PHST- 2016/12/28 06:00 [entrez]
AID - S0009-2797(16)30435-5 [pii]
AID - 10.1016/j.cbi.2016.12.014 [doi]
PST - ppublish
SO  - Chem Biol Interact. 2017 Feb 1;263:46-54. doi: 10.1016/j.cbi.2016.12.014. Epub 
      2016 Dec 23.