PMID- 28028895
OWN - NLM
STAT- MEDLINE
DCOM- 20171103
LR  - 20181202
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 105
IP  - 4
DP  - 2017 Apr
TI  - Characterization of Sox9-overexpressing human umbilical cord blood-derived 
      mesenchymal stem cells-based engineered cartilage both in vitro and in vivo.
PG  - 1150-1155
LID - 10.1002/jbm.a.35989 [doi]
AB  - The source of seed cells is a critical factor for tissue engineering. The goal of 
      this study was to evaluate the chondrogenesis of Sox9-overexpressing human 
      umbilical cord mesenchymal stem cells (hUCMSCs) seeded onto bone matrix gelatin 
      (BMG)/fibrin hybrid scaffolds both in vitro and in vivo. hUCMSCs were stably 
      transfected with Sox9-expressing plasmid and grown on the three-dimensional 
      BMG/fibrin hybrid scaffold for 8 weeks. Scanning electron microscopy and 
      histochemistry were performed. The hUCMSC-loaded scaffolds were implanted into 
      the subcutaneous layer of immunocompetent rats and chondrogenesis and host immune 
      responses were monitored for 8 weeks. We found that hUCMSCs spread well and 
      proliferated from 2 weeks after culturing. They produced abundant 
      glycosaminoglycans and collagen II. At 8 weeks after implanting into rats, the 
      hUCMSCs on the scaffolds formed cartilage-like tissue and displayed positive 
      staining for toluidine blue, safranin O, Masson's trichrome, and collagen II. No 
      significant changes in serum levels of lgG, lgA, lgM, C3, and C4 were observed 
      after implantation of the hUCMSC-loaded scaffolds. Xenogeneic implantation of 
      Sox9-overexpressing hUCMSCs embedded in the BMG/fibrin scaffolds promotes the 
      formation of cartilage-like tissue without inducing evident host immune response. 
      Therefore, Sox9-overexpressing hUCMSCs represent a promising cell candidate for 
      cartilage tissue engineering. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res 
      Part A: 105A: 1150-1155, 2017.
CI  - (c) 2017 Wiley Periodicals, Inc.
FAU - Li, Xiao-Li
AU  - Li XL
AD  - Department of Dermatology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University, Xi'an, 710004, China.
FAU - Zhang, Jun
AU  - Zhang J
AD  - Department of Otolaryngology, The Affiliated Hospital of Yan'an University, 
      Yan'an, China.
FAU - Luo, Hua-Nan
AU  - Luo HN
AD  - Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital, Xi'an Jiaotong University, Xi'an, 710004, China.
FAU - Zhao, Xiao-Yan
AU  - Zhao XY
AD  - Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital, Xi'an Jiaotong University, Xi'an, 710004, China.
FAU - Zhang, A-Ling
AU  - Zhang AL
AD  - Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital, Xi'an Jiaotong University, Xi'an, 710004, China.
FAU - Wang, Zheng-Hui
AU  - Wang ZH
AD  - Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital, Xi'an Jiaotong University, Xi'an, 710004, China.
LA  - eng
PT  - Journal Article
DEP - 20170209
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (SOX9 Transcription Factor)
RN  - 0 (SOX9 protein, human)
SB  - IM
MH  - Animals
MH  - Cartilage/cytology/*metabolism
MH  - Fetal Blood/cytology/*metabolism
MH  - Heterografts
MH  - Humans
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - SOX9 Transcription Factor/*biosynthesis
MH  - Time Factors
MH  - *Tissue Engineering
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - chondrogenesis
OT  - immune rejection
OT  - mesenchymal stem cells
OT  - tissue engineering
EDAT- 2016/12/29 06:00
MHDA- 2017/11/04 06:00
CRDT- 2016/12/29 06:00
PHST- 2016/10/31 00:00 [received]
PHST- 2016/12/09 00:00 [revised]
PHST- 2016/12/21 00:00 [accepted]
PHST- 2016/12/29 06:00 [pubmed]
PHST- 2017/11/04 06:00 [medline]
PHST- 2016/12/29 06:00 [entrez]
AID - 10.1002/jbm.a.35989 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2017 Apr;105(4):1150-1155. doi: 10.1002/jbm.a.35989. Epub 
      2017 Feb 9.