PMID- 28029738
OWN - NLM
STAT- MEDLINE
DCOM- 20171121
LR  - 20240104
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 89
IP  - 6
DP  - 2017 Mar
TI  - DRO1 influences root system architecture in Arabidopsis and Prunus species.
PG  - 1093-1105
LID - 10.1111/tpj.13470 [doi]
AB  - Roots provide essential uptake of water and nutrients from the soil, as well as 
      anchorage and stability for the whole plant. Root orientation, or angle, is an 
      important component of the overall architecture and depth of the root system; 
      however, little is known about the genetic control of this trait. Recent reports 
      in Oryza sativa (rice) identified a role for DEEPER ROOTING 1 (DRO1) in 
      influencing the orientation of the root system, leading to positive changes in 
      grain yields under water-limited conditions. Here we found that DRO1 and 
      DRO1-related genes are present across diverse plant phyla, and fall within the 
      IGT gene family. The IGT family also includes TAC1 and LAZY1, which are known to 
      affect the orientation of lateral shoots. Consistent with a potential role in 
      root development, DRO1 homologs in Arabidopsis and peach showed root-specific 
      expression. Promoter-reporter constructs revealed that AtDRO1 is predominantly 
      expressed in both the root vasculature and root tips, in a distinct developmental 
      pattern. Mutation of AtDRO1 led to more horizontal lateral root angles. 
      Overexpression of AtDRO1 under a constitutive promoter resulted in steeper 
      lateral root angles, as well as shoot phenotypes including upward leaf curling, 
      shortened siliques and narrow lateral branch angles. A conserved C-terminal 
      EAR-like motif found in IGT genes was required for these ectopic phenotypes. 
      Overexpression of PpeDRO1 in Prunus domestica (plum) led to deeper-rooting 
      phenotypes. Collectively, these data indicate a potential application for 
      DRO1-related genes to alter root architecture for drought avoidance and improved 
      resource use.
CI  - Published 2016. This article is a U.S. Government work and is in the public 
      domain in the USA.
FAU - Guseman, Jessica M
AU  - Guseman JM
AD  - USDA-ARS Appalachian Fruit Research Station, 2217 Wiltshire Rd., Kearneysville, 
      WV, 25430, USA.
FAU - Webb, Kevin
AU  - Webb K
AD  - USDA-ARS Appalachian Fruit Research Station, 2217 Wiltshire Rd., Kearneysville, 
      WV, 25430, USA.
FAU - Srinivasan, Chinnathambi
AU  - Srinivasan C
AD  - USDA-ARS Appalachian Fruit Research Station, 2217 Wiltshire Rd., Kearneysville, 
      WV, 25430, USA.
FAU - Dardick, Chris
AU  - Dardick C
AD  - USDA-ARS Appalachian Fruit Research Station, 2217 Wiltshire Rd., Kearneysville, 
      WV, 25430, USA.
LA  - eng
SI  - GENBANK/AY735558.1
SI  - GENBANK/DQ487489.1
SI  - GENBANK/AY954756.1
SI  - GENBANK/KB639030.1
SI  - GENBANK/KB639097.1
PT  - Journal Article
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Plant Proteins)
SB  - IM
MH  - Arabidopsis/genetics/*metabolism/physiology
MH  - Gravitropism/genetics/physiology
MH  - Plant Proteins/genetics/*metabolism
MH  - Plant Roots/genetics/*metabolism/physiology
MH  - Plants, Genetically Modified/genetics/growth & development/metabolism
MH  - Promoter Regions, Genetic/*genetics
MH  - Prunus/genetics/*metabolism/physiology
OTO - NOTNLM
OT  - Arabidopsis
OT  - IGT family
OT  - LAZY1
OT  - Prunus
OT  - TAC1
OT  - gravitropism
OT  - root orientation
OT  - root system architecture
EDAT- 2016/12/29 06:00
MHDA- 2017/11/29 06:00
CRDT- 2016/12/29 06:00
PHST- 2016/06/15 00:00 [received]
PHST- 2016/12/12 00:00 [revised]
PHST- 2016/12/16 00:00 [accepted]
PHST- 2016/12/29 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/12/29 06:00 [entrez]
AID - 10.1111/tpj.13470 [doi]
PST - ppublish
SO  - Plant J. 2017 Mar;89(6):1093-1105. doi: 10.1111/tpj.13470.