PMID- 28032743
OWN - NLM
STAT- MEDLINE
DCOM- 20181212
LR  - 20181212
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 9
IP  - 4
DP  - 2017 Feb 1
TI  - Sustained Local Release of NGF from a Chitosan-Sericin Composite Scaffold for 
      Treating Chronic Nerve Compression.
PG  - 3432-3444
LID - 10.1021/acsami.6b14691 [doi]
AB  - Chronic nerve compression (CNC), a common form of peripheral nerve injury, always 
      leads to chronic peripheral nerve pain and dysfunction. Current available 
      treatments for CNC are ineffective as they usually aim to alleviate symptoms at 
      the acute phase with limited capability toward restoring injured nerve function. 
      New approaches for effective recovery of CNC injury are highly desired. Here we 
      report for the first time a tissue-engineered approach for the repair of CNC. A 
      genipin cross-linked chitosan-sericin 3D scaffold for delivering nerve growth 
      factor (NGF) was designed and fabricated. This scaffold combines the advantages 
      of both chitosan and sericin, such as high porosity, adjustable mechanical 
      properties and swelling ratios, the ability of supporting Schwann cells growth, 
      and improving nerve regeneration. The degradation products of the composite 
      scaffold upregulate the mRNA levels of the genes important for facilitating nerve 
      function recovery, including glial-derived neurotrophic factor (GDNF), early 
      growth response 2 (EGR2), and neural cell adhesion molecule (NCAM) in Schwann 
      cells, while down-regulating two inflammatory genes' mRNA levels in macrophages, 
      tumor necrosis factor alpha (TNF-alpha), and interleukin-1 beta (IL-1beta). Importantly, 
      our tissue-engineered strategy achieves significant nerve functional recovery in 
      a preclinical CNC animal model by decreasing neuralgia, improving nerve 
      conduction velocity (NCV), accelerating microstructure restoration, and 
      attenuating gastrocnemius muscles dystrophy. Together, this work suggests a 
      promising clinical alternative for treating chronic peripheral nerve compression 
      injury.
FAU - Zhang, Lei
AU  - Zhang L
AD  - Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, 
      Tongji Medical College, Huazhong University of Science and Technology , Jiefang 
      Avenue 1277, Wuhan, China 430022.
AD  - Department of Clinical Laboratory, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology , Wuhan, China 430022.
FAU - Yang, Wen
AU  - Yang W
AD  - Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, 
      Tongji Medical College, Huazhong University of Science and Technology , Jiefang 
      Avenue 1277, Wuhan, China 430022.
FAU - Tao, Kaixiong
AU  - Tao K
AD  - Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology , Wuhan, China 430022.
FAU - Song, Yu
AU  - Song Y
AD  - Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, 
      Tongji Medical College, Huazhong University of Science and Technology , Jiefang 
      Avenue 1277, Wuhan, China 430022.
FAU - Xie, Hongjian
AU  - Xie H
AD  - Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, 
      Tongji Medical College, Huazhong University of Science and Technology , Jiefang 
      Avenue 1277, Wuhan, China 430022.
FAU - Wang, Jian
AU  - Wang J
AD  - Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, 
      Tongji Medical College, Huazhong University of Science and Technology , Jiefang 
      Avenue 1277, Wuhan, China 430022.
FAU - Li, Xiaolin
AU  - Li X
AD  - Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, 
      Tongji Medical College, Huazhong University of Science and Technology , Jiefang 
      Avenue 1277, Wuhan, China 430022.
FAU - Shuai, Xiaoming
AU  - Shuai X
AD  - Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology , Wuhan, China 430022.
FAU - Gao, Jinbo
AU  - Gao J
AD  - Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology , Wuhan, China 430022.
FAU - Chang, Panpan
AU  - Chang P
AD  - Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, 
      Tongji Medical College, Huazhong University of Science and Technology , Jiefang 
      Avenue 1277, Wuhan, China 430022.
FAU - Wang, Guobin
AU  - Wang G
AD  - Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology , Wuhan, China 430022.
FAU - Wang, Zheng
AU  - Wang Z
AD  - Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, 
      Tongji Medical College, Huazhong University of Science and Technology , Jiefang 
      Avenue 1277, Wuhan, China 430022.
AD  - Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology , Wuhan, China 430022.
FAU - Wang, Lin
AU  - Wang L
AUID- ORCID: 0000-0001-5716-6587
AD  - Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, 
      Tongji Medical College, Huazhong University of Science and Technology , Jiefang 
      Avenue 1277, Wuhan, China 430022.
AD  - Department of Clinical Laboratory, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology , Wuhan, China 430022.
LA  - eng
PT  - Journal Article
DEP - 20170120
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Sericins)
RN  - 9012-76-4 (Chitosan)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
EIN - ACS Appl Mater Interfaces. 2020 Jan 15;12(2):3276. PMID: 31904209
MH  - Animals
MH  - Chitosan
MH  - Nerve Growth Factor/*chemistry
MH  - Nerve Regeneration
MH  - Rats, Sprague-Dawley
MH  - Schwann Cells
MH  - Sericins
OTO - NOTNLM
OT  - NGF
OT  - chitosan-sericin scaffold
OT  - chronic nerve compression
OT  - functional restoration
OT  - tissue engineering approach
EDAT- 2016/12/30 06:00
MHDA- 2018/12/13 06:00
CRDT- 2016/12/30 06:00
PHST- 2016/12/30 06:00 [pubmed]
PHST- 2018/12/13 06:00 [medline]
PHST- 2016/12/30 06:00 [entrez]
AID - 10.1021/acsami.6b14691 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2017 Feb 1;9(4):3432-3444. doi: 
      10.1021/acsami.6b14691. Epub 2017 Jan 20.