PMID- 28032995
OWN - NLM
STAT- MEDLINE
DCOM- 20170428
LR  - 20191210
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 65
IP  - 3
DP  - 2017 Jan 25
TI  - Comprehensive Profiling of Phytohormones in Honey by Sequential Liquid-Liquid 
      Extraction Coupled with Liquid Chromatography-Mass Spectrometry.
PG  - 575-585
LID - 10.1021/acs.jafc.6b04234 [doi]
AB  - Honey exhibits various nutritional and medicinal functions, which are highly 
      related to the active components; thus, the exploration of new compounds in honey 
      is of great importance. Because honey is a byproduct of flower nectar, which is 
      rich in phytohormones, the existence of phytohormones in honey is anticipated. In 
      this research, a method for comprehensive profiling of 49 phytohormones in honey 
      was developed by sequential liquid-liquid extraction (LLE) coupled with liquid 
      chromatography-tandem mass spectrometry (LC-MS/MS). Good linearities for 49 
      phytohormones were obtained with correlation coefficients (R) larger than 0.9913. 
      The limits of detection (LODs) were in the range of 0.2-628.2 pg/mL. Satisfied 
      reproducibility and reliability were achieved by evaluation of the intra- and 
      interday precisions with relative standard deviations (RSDs) less than 15.8% and 
      relative recoveries ranging from 80.4 to 123.7%. The method was further applied 
      to analyze the phytohormones in 14 monofloral raw honey samples and 3 commercial 
      honey samples. The existence of 34 phytohormones was confirmed, including 14 
      cytokinins (CKs), 8 gibberellins (GAs), 5 brassinosteroids (BRs), indole-3-acetic 
      acid (IAA), indole-3-butyric acid (IBA), abscisic acid (ABA), salicylic acid 
      (SA), jasmonic acid (JA), jasmonoyl-leucine (JA-Leu), and jasmonoyl-phenylalanine 
      (JA-Phe). In addition, the content and species of phytohormones varies in 
      different kinds of honey. The study is beneficial to fully illustrate the 
      phytohormone profile of honey and contributive to elucidate the mechanism of its 
      nutritional and medicinal functions.
FAU - Wang, Qing
AU  - Wang Q
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University , Wuhan, Hubei 430072, 
      People's Republic of China.
FAU - Cai, Wen-Jing
AU  - Cai WJ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University , Wuhan, Hubei 430072, 
      People's Republic of China.
FAU - Yu, Lei
AU  - Yu L
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University , Wuhan, Hubei 430072, 
      People's Republic of China.
FAU - Ding, Jun
AU  - Ding J
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University , Wuhan, Hubei 430072, 
      People's Republic of China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AUID- ORCID: 0000-0003-1107-5385
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University , Wuhan, Hubei 430072, 
      People's Republic of China.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20170112
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Plant Growth Regulators)
SB  - IM
MH  - Chromatography, Liquid/*methods
MH  - Honey/*analysis
MH  - Liquid-Liquid Extraction/*methods
MH  - Molecular Structure
MH  - Plant Growth Regulators/*chemistry/*isolation & purification
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
OTO - NOTNLM
OT  - honey
OT  - liquid-liquid extraction
OT  - mass spectrometry
OT  - nutritional and medicinal function
OT  - phytohormones
EDAT- 2016/12/30 06:00
MHDA- 2017/04/30 06:00
CRDT- 2016/12/30 06:00
PHST- 2016/12/30 06:00 [pubmed]
PHST- 2017/04/30 06:00 [medline]
PHST- 2016/12/30 06:00 [entrez]
AID - 10.1021/acs.jafc.6b04234 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2017 Jan 25;65(3):575-585. doi: 10.1021/acs.jafc.6b04234. Epub 
      2017 Jan 12.