PMID- 28033431
OWN - NLM
STAT- MEDLINE
DCOM- 20170705
LR  - 20220409
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 12
DP  - 2016
TI  - Integrated Analysis of Long Noncoding RNA and mRNA Expression Profile in Advanced 
      Laryngeal Squamous Cell Carcinoma.
PG  - e0169232
LID - 10.1371/journal.pone.0169232 [doi]
LID - e0169232
AB  - Long non-coding RNA (lncRNA) plays an important role in tumorigenesis. However, 
      the expression pattern and function of lncRNAs in laryngeal squamous cell 
      carcinoma (LSCC) are still unclear. To investigate the aberrantly expressed 
      lncRNAs and mRNAs in advanced LSCC, we screened lncRNA and mRNA expression 
      profiles in 9 pairs of primary Stage IVA LSCC tissues and adjacent non-neoplastic 
      tissues by lncRNA and mRNA integrated microarrays. Gene Ontology and pathway 
      analysis were performed to find out the significant function and pathway of the 
      differentially expressed mRNAs, gene-gene functional interaction network and 
      ceRNA network were constructed to select core mRNAs, and lncRNA-mRNA expression 
      correlation network was built to identify the interactions between lncRNA and 
      mRNA. qRT-PCR was performed to further validate the expressions of selected 
      lncRNAs and mRNAs in advanced LSCC. We found 1459 differentially expressed 
      lncRNAs and 2381 differentially expressed mRNAs, including 846 up-regulated 
      lncRNAs and 613 down-regulated lncRNAs, 1542 up-regulated mRNAs and 839 
      down-regulated mRNAs. The mRNAs ITGB1, HIF1A, and DDIT4 were selected as core 
      mRNAs, which are mainly involved in biological processes, such as matrix 
      organization, cell cycle, adhesion, and metabolic pathway. LncRNA-mRNA expression 
      correlation network showed LncRNA NR_027340, MIR31HG were positively correlated 
      with ITGB1, HIF1A respectively. LncRNA SOX2-OT was negatively correlated with 
      DDIT4. qRT-PCR further validated the expression of these lncRNAs and mRNAs. The 
      work provides convincing evidence that the identified lncRNAs and mRNAs are 
      potential biomarkers in advanced LSCC for further future studies.
FAU - Feng, Ling
AU  - Feng L
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, China.
AD  - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of 
      Education, Beijing, China.
FAU - Wang, Ru
AU  - Wang R
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, China.
AD  - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of 
      Education, Beijing, China.
FAU - Lian, Meng
AU  - Lian M
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, China.
AD  - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of 
      Education, Beijing, China.
FAU - Ma, Hongzhi
AU  - Ma H
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, China.
AD  - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of 
      Education, Beijing, China.
FAU - He, Ning
AU  - He N
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, China.
AD  - Department of Head and Neck Surgery, The People's Hospital of Guangxi Zhuang 
      Autonomous Region, Nanning, Guangxi, China.
FAU - Liu, Honggang
AU  - Liu H
AD  - Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing, 
      China.
FAU - Wang, Haizhou
AU  - Wang H
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, China.
AD  - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of 
      Education, Beijing, China.
FAU - Fang, Jugao
AU  - Fang J
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, China.
AD  - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of 
      Education, Beijing, China.
AD  - Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20161229
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics/*pathology
MH  - Computational Biology/*methods
MH  - Female
MH  - *Gene Expression Profiling
MH  - Gene Ontology
MH  - Gene Regulatory Networks
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - RNA, Long Noncoding/*genetics
MH  - RNA, Messenger/genetics
PMC - PMC5199101
COIS- The authors have declared that no competing interests exist.
EDAT- 2016/12/30 06:00
MHDA- 2017/07/06 06:00
PMCR- 2016/12/29
CRDT- 2016/12/30 06:00
PHST- 2016/09/27 00:00 [received]
PHST- 2016/12/13 00:00 [accepted]
PHST- 2016/12/30 06:00 [entrez]
PHST- 2016/12/30 06:00 [pubmed]
PHST- 2017/07/06 06:00 [medline]
PHST- 2016/12/29 00:00 [pmc-release]
AID - PONE-D-16-38119 [pii]
AID - 10.1371/journal.pone.0169232 [doi]
PST - epublish
SO  - PLoS One. 2016 Dec 29;11(12):e0169232. doi: 10.1371/journal.pone.0169232. 
      eCollection 2016.