PMID- 2803404
OWN - NLM
STAT- MEDLINE
DCOM- 19891127
LR  - 20131121
IS  - 0904-1850 (Print)
IS  - 0904-1850 (Linking)
VI  - 6
DP  - 1989 Jun
TI  - Oxidative metabolism of human peripheral blood eosinophils and neutrophils: H2O2 
      production after stimulation with phorbol myristate acetate and immune complexes.
PG  - 435s-440s
AB  - Eosinophils like neutrophils possess an oxidative metabolism which is activated 
      by phagocytosis and cell membrane activation. Since eosinophils may take part in 
      inflammatory reactions by releasing oxygen species and have membrane receptors 
      for immunoglobulins, immune complexes may activate their respiratory burst. We 
      compared the ability of phorbol myristate acetate (PMA) and immunoglobulin G 
      (IgG) and immunoglobulin E (IgE) immune complexes to stimulate hydrogen peroxide 
      (H2O2) release from circulating eosinophils and neutrophils. PMA was able to 
      stimulate a significant respiratory burst in both cell types, PMA-induced H2O2 
      production being higher in eosinophils than in neutrophils (p less than 0.05). 
      Similar results were obtained when the cells were stimulated by IgG (p less than 
      0.05). In contrast, aggregated human myeloma IgE induced a detectable H2O2 
      release only from peripheral blood eosinophils. We conclude that H2O2 release 
      from eosinophils may play a role in parenchymal injury associated with 
      hypereosinophilic disorders and the presence of immune complexes at sites of 
      disease activity.
FAU - Rossi, G A
AU  - Rossi GA
AD  - First Division of Pneumology, San Martino Hospital, Genoa, Italy.
FAU - Sacco, O
AU  - Sacco O
FAU - Vassallo, F
AU  - Vassallo F
FAU - Lantero, S
AU  - Lantero S
FAU - Morelli, A
AU  - Morelli A
FAU - Benatti, U
AU  - Benatti U
FAU - Damiani, G
AU  - Damiani G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur Respir J Suppl
JT  - The European respiratory journal. Supplement
JID - 8910681
RN  - 0 (Antigen-Antibody Complex)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adult
MH  - Antigen-Antibody Complex/*physiology
MH  - Eosinophils/drug effects/*metabolism
MH  - Female
MH  - Humans
MH  - Hydrogen Peroxide/metabolism
MH  - Male
MH  - Neutrophils/drug effects/*metabolism
MH  - Oxygen Consumption/*drug effects
MH  - Tetradecanoylphorbol Acetate/*pharmacology
EDAT- 1989/06/01 00:00
MHDA- 1989/06/01 00:01
CRDT- 1989/06/01 00:00
PHST- 1989/06/01 00:00 [pubmed]
PHST- 1989/06/01 00:01 [medline]
PHST- 1989/06/01 00:00 [entrez]
PST - ppublish
SO  - Eur Respir J Suppl. 1989 Jun;6:435s-440s.