PMID- 28041913
OWN - NLM
STAT- MEDLINE
DCOM- 20170410
LR  - 20181113
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Print)
IS  - 0141-8130 (Linking)
VI  - 97
DP  - 2017 Apr
TI  - A three-dimensional in vitro model to demonstrate the haptotactic effect of 
      monocyte chemoattractant protein-1 on atherosclerosis-associated monocyte 
      migration.
PG  - 141-147
LID - S0141-8130(16)31760-3 [pii]
LID - 10.1016/j.ijbiomac.2016.12.072 [doi]
AB  - Monocyte transendothelial migration is a multi-step process critical for the 
      initiation and development of atherosclerosis. The chemokine monocyte 
      chemoattractant protein-1 (MCP-1) is overexpressed during atheroma and its 
      concentration gradients in the extracellular matrix (ECM) is critical for the 
      transendothelial recruitment of monocytes. Based on prior observations, we 
      hypothesize that both free and bound gradients of MCP-1 within the ECM are 
      involved in directing monocyte migration. The interaction between a 
      three-dimensional (3D), cell-free, collagen matrix and MCP-1; and its effect on 
      monocyte migration was measured in this study. Our results showed such an 
      interaction existed between MCP-1 and collagen, as 26% of the total MCP-1 added 
      to the collagen matrix was bound to the matrix after extensive washes. We also 
      characterized the collagen-MCP-1 interaction using biophysical techniques. The 
      treatment of the collagen matrix with MCP-1 lead to increased monocyte migration, 
      and this phenotype was abrogated by treating the matrix with an anti-MCP-1 
      antibody. Thus, our results indicate a binding interaction between MCP-1 and the 
      collagen matrix, which could elicit a haptotactic effect on monocyte migration. A 
      better understanding of such mechanisms controlling monocyte migration will help 
      identify target cytokines and lead to the development of better anti-inflammatory 
      therapeutic strategies.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Ghousifam, Neda
AU  - Ghousifam N
AD  - School of Chemical Engineering at Oklahoma State University, Stillwater, OK, 
      74078, USA.
FAU - Mortazavian, Hamid
AU  - Mortazavian H
AD  - Department of Chemistry at Oklahoma State University, Stillwater, OK, 74078, USA.
FAU - Bhowmick, Rudra
AU  - Bhowmick R
AD  - School of Chemical Engineering at Oklahoma State University, Stillwater, OK, 
      74078, USA.
FAU - Vasquez, Yolanda
AU  - Vasquez Y
AD  - Department of Chemistry at Oklahoma State University, Stillwater, OK, 74078, USA.
FAU - Blum, Frank D
AU  - Blum FD
AD  - Department of Chemistry at Oklahoma State University, Stillwater, OK, 74078, USA.
FAU - Gappa-Fahlenkamp, Heather
AU  - Gappa-Fahlenkamp H
AD  - School of Chemical Engineering at Oklahoma State University, Stillwater, OK, 
      74078, USA. Electronic address: heather.fahlenkamp@okstate.edu.
LA  - eng
GR  - R15 EB009527/EB/NIBIB NIH HHS/United States
PT  - Journal Article
DEP - 20161229
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Chemokine CCL2)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Cell Adhesion
MH  - *Cell Movement
MH  - Chemokine CCL2/*metabolism
MH  - Collagen/*metabolism
MH  - Extracellular Matrix/metabolism
MH  - Humans
MH  - *Models, Biological
MH  - Monocytes/*pathology
MH  - Plaque, Atherosclerotic/*metabolism/*pathology
MH  - Temperature
PMC - PMC5413302
MID - NIHMS843105
OTO - NOTNLM
OT  - Atherosclerosis
OT  - Monocyte chemoattractant protein-1
OT  - Monocyte migration
EDAT- 2017/01/04 06:00
MHDA- 2017/04/11 06:00
PMCR- 2018/04/01
CRDT- 2017/01/03 06:00
PHST- 2016/09/23 00:00 [received]
PHST- 2016/12/23 00:00 [revised]
PHST- 2016/12/28 00:00 [accepted]
PHST- 2017/01/04 06:00 [pubmed]
PHST- 2017/04/11 06:00 [medline]
PHST- 2017/01/03 06:00 [entrez]
PHST- 2018/04/01 00:00 [pmc-release]
AID - S0141-8130(16)31760-3 [pii]
AID - 10.1016/j.ijbiomac.2016.12.072 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2017 Apr;97:141-147. doi: 10.1016/j.ijbiomac.2016.12.072. 
      Epub 2016 Dec 29.