PMID- 28044285
OWN - NLM
STAT- MEDLINE
DCOM- 20180226
LR  - 20180426
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1547
DP  - 2017
TI  - Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Patterning 
      Capture Antibodies Using Microcontact Printing and Dry-Film Resists.
PG  - 37-47
LID - 10.1007/978-1-4939-6734-6_3 [doi]
AB  - The miniaturization of immunoassays using microfluidic devices is attractive for 
      many applications, but an important challenge remains the patterning of capture 
      antibodies (cAbs) on the surface of microfluidic structures. Here, we describe 
      how to pattern cAbs on planar poly(dimethylsiloxane) (PDMS) stamps and how to 
      microcontact print the cAbs on a dry-film resist (DFR). DFRs are new types of 
      photoresists having excellent chemical resistance and good mechanical, adhesive, 
      and optical properties. Instead of being liquid photoresists, DFRs are thin 
      layers that are easy to handle, cut, photo-pattern, and laminate over surfaces. 
      We show how to perform a simple fluorescence immunoassay using anti-biotin cAbs 
      patterned on a 50-mum-thick DF-1050 DFR, Atto 647N-biotin analytes, and 
      capillary-driven chips fabricated in silicon.
FAU - Temiz, Yuksel
AU  - Temiz Y
AD  - IBM Research GmbH, Saumerstrasse 4, 8803, Ruschlikon, Switzerland.
FAU - Lovchik, Robert D
AU  - Lovchik RD
AD  - IBM Research GmbH, Saumerstrasse 4, 8803, Ruschlikon, Switzerland.
FAU - Delamarche, Emmanuel
AU  - Delamarche E
AD  - IBM Research GmbH, Saumerstrasse 4, 8803, Ruschlikon, Switzerland. 
      emd@zurich.ibm.com.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antibodies)
SB  - IM
MH  - Antibodies/immunology
MH  - Equipment Design
MH  - Fluorescent Antibody Technique
MH  - Immunoassay/*instrumentation/methods
MH  - Microfluidic Analytical Techniques/*instrumentation
MH  - Microfluidics/*instrumentation/methods
MH  - Microscopy, Fluorescence
OTO - NOTNLM
OT  - Dry-film resist
OT  - Immunoassay
OT  - Microcontact printing
OT  - Microfluidics
OT  - PDMS
OT  - Patterning antibodies
EDAT- 2017/01/04 06:00
MHDA- 2018/02/27 06:00
CRDT- 2017/01/04 06:00
PHST- 2017/01/04 06:00 [entrez]
PHST- 2017/01/04 06:00 [pubmed]
PHST- 2018/02/27 06:00 [medline]
AID - 10.1007/978-1-4939-6734-6_3 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1547:37-47. doi: 10.1007/978-1-4939-6734-6_3.