PMID- 28049953
OWN - NLM
STAT- MEDLINE
DCOM- 20170203
LR  - 20181202
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 40
IP  - 1
DP  - 2017
TI  - Cobalt Chloride Induces Expression and Function of Breast Cancer Resistance 
      Protein (BCRP/ABCG2) in Human Renal Proximal Tubular Epithelial Cell Line HK-2.
PG  - 82-87
LID - 10.1248/bpb.b16-00684 [doi]
AB  - The human breast cancer resistance protein (BCRP/ABCG2), a member of the 
      ATP-binding cassette transporter family, is a drug transporter restricting 
      absorption and enhancing excretion of many compounds including anticancer drugs. 
      The cis-regulatory elements in the BCRP promoter include a hypoxia response 
      element, i.e., the DNA binding site for hypoxia-inducible factor-1 (HIF-1). In 
      this study, we investigated the effect of cobalt chloride, a chemical inducer of 
      HIF-1alpha, on the expression and function of BCRP in human renal proximal tubular 
      cell line HK-2. Cobalt chloride treatment significantly increased the mRNA 
      expression of not only glucose transporter 1 (GLUT1), a typical HIF-1 target gene 
      mRNA, but also ABCG2 mRNA in HK-2 cells. The BCRP inhibitor Ko143-sensitive 
      accumulation of BCRP substrates such as Hoechst33342 and mitoxantrone was 
      significantly enhanced by cobalt chloride treatment. In addition, treatment with 
      cobalt chloride significantly increased the Ko143-sensitive accumulation of 
      fluorescein isothiocyanate-labeled methotrexate in HK-2 cells. Furthermore, 
      cobalt chloride treatment attenuated the cytotoxicity induced by mitoxantrone and 
      methotrexate, which might be, at least in part, due to the increase in 
      BCRP-mediated transport activity via HIF-1 activation. These findings indicate 
      that HIF-1 activation protects renal proximal tubular cells against BCRP 
      substrate-induced cytotoxicity by enhancing the expression and function of BCRP 
      in renal proximal tubular cells.
FAU - Nishihashi, Katsuki
AU  - Nishihashi K
AD  - Laboratory of Pharmaceutics, Osaka University of Pharmaceutical Sciences.
FAU - Kawashima, Kei
AU  - Kawashima K
FAU - Nomura, Takami
AU  - Nomura T
FAU - Urakami-Takebayashi, Yumiko
AU  - Urakami-Takebayashi Y
FAU - Miyazaki, Makoto
AU  - Miyazaki M
FAU - Takano, Mikihisa
AU  - Takano M
FAU - Nagai, Junya
AU  - Nagai J
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (ABCG2 protein, human)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 2)
RN  - 0 (Glucose Transporter Type 1)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 3G0H8C9362 (Cobalt)
RN  - BZ114NVM5P (Mitoxantrone)
RN  - EVS87XF13W (cobaltous chloride)
RN  - YL5FZ2Y5U1 (Methotrexate)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 2/genetics/*metabolism
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Cobalt/*pharmacology
MH  - Epithelial Cells/*drug effects/metabolism
MH  - Glucose Transporter Type 1/genetics
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/genetics/metabolism
MH  - Methotrexate/pharmacology
MH  - Mitoxantrone/pharmacology
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - RNA, Messenger/metabolism
EDAT- 2017/01/05 06:00
MHDA- 2017/02/06 06:00
CRDT- 2017/01/05 06:00
PHST- 2017/01/05 06:00 [entrez]
PHST- 2017/01/05 06:00 [pubmed]
PHST- 2017/02/06 06:00 [medline]
AID - 10.1248/bpb.b16-00684 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2017;40(1):82-87. doi: 10.1248/bpb.b16-00684.