PMID- 28050866
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
DP  - 2017 Jan 3
TI  - MicroRNA-638 inhibits cell proliferation by targeting suppress PIM1 expression in 
      human osteosarcoma.
LID - 10.1007/s13277-016-5379-1 [doi]
AB  - MicroRNAs (miRNAs) are a type of small noncoding RNAs that often play important 
      roles in carcinogenesis, but the carcinogenic mechanism of miRNAs is still 
      unclear. This study will investigate the functions and the mechanism of miR-638 
      in osteosarcoma (OS). The expression of miR-638 in OS and the DNA copy number of 
      miR-638 were detected by real-time PCR. The effect of miR-638 on cell 
      proliferation was measured by CCK8 assay. Different assays, including 
      bioinformatics algorithms, luciferase report assay, and Western blotting, were 
      used to identify the target gene proviral integration site for Moloney murine 
      leukemia virus 1 (PIM1) of miR-638 in OS. The expression of PIM1 in clinical OS 
      tissues was also validated by immunohistochemical assay. From this research, we 
      found that miR-638 was downregulated in OS tissues compared with corresponding 
      noncancerous tissues (NCTs), and the DNA copy number of miR-638 was lower in OS 
      than in NCTs, which may induce the corresponding downregulation of miR-638 in OS. 
      Ectopic expression of miR-638 inhibited OS cell growth in vitro. Subsequently, we 
      identified that PIM1 is the downstream target gene of miR-638 in OS cells, and 
      silencing PIM1 expression phenocopied the inhibitory effect of miR-638 on OS cell 
      proliferation. Furthermore, we observed that PIM1 was overexpressed in OS 
      tissues, and high expression of PIM1 in OS predicted poor overall survival. In 
      summary, we revealed that miR-638 functions as a tumor suppressor through 
      inhibiting PIM1 expression in OS.
FAU - Wang, Xiao-Xu
AU  - Wang XX
AD  - Department of Joint Surgery, the Second Affiliated Hospital, University of South 
      China, 35 Jiefang Road, Hengyang, Hunan, People's Republic of China.
FAU - Liu, Jue
AU  - Liu J
AD  - Department of Dobstertics and Gynecology, the Second Affiliated Hospital, 
      University of South China, Hengyang, Hunan, People's Republic of China.
FAU - Tang, Yi-Min
AU  - Tang YM
AD  - Department of Nursing, the First Affiliated Hospital, University of South China, 
      Hengyang, Hunan, People's Republic of China.
FAU - Hong, Liang
AU  - Hong L
AD  - Department of Joint Surgery, the Second Affiliated Hospital, University of South 
      China, 35 Jiefang Road, Hengyang, Hunan, People's Republic of China.
FAU - Zeng, Zhi
AU  - Zeng Z
AD  - Department of Joint Surgery, the Second Affiliated Hospital, University of South 
      China, 35 Jiefang Road, Hengyang, Hunan, People's Republic of China.
FAU - Tan, Guang-Hua
AU  - Tan GH
AD  - Department of Joint Surgery, the Second Affiliated Hospital, University of South 
      China, 35 Jiefang Road, Hengyang, Hunan, People's Republic of China. 
      fueryisheng@163.com.
LA  - eng
PT  - Journal Article
DEP - 20170103
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
OTO - NOTNLM
OT  - Osteosarcoma
OT  - PIM1
OT  - Proliferation
OT  - miR-638
EDAT- 2017/01/05 06:00
MHDA- 2017/01/05 06:00
CRDT- 2017/01/05 06:00
PHST- 2016/03/31 00:00 [received]
PHST- 2016/09/09 00:00 [accepted]
PHST- 2017/01/05 06:00 [entrez]
PHST- 2017/01/05 06:00 [pubmed]
PHST- 2017/01/05 06:00 [medline]
AID - 10.1007/s13277-016-5379-1 [pii]
AID - 10.1007/s13277-016-5379-1 [doi]
PST - aheadofprint
SO  - Tumour Biol. 2017 Jan 3. doi: 10.1007/s13277-016-5379-1.