PMID- 28053145
OWN - NLM
STAT- MEDLINE
DCOM- 20180522
LR  - 20180522
IS  - 1460-2709 (Electronic)
IS  - 1369-3786 (Linking)
VI  - 55
IP  - 7
DP  - 2017 Oct 1
TI  - Study of differential expression of miRNAs in lung tissue of mice submitted to 
      experimental infection by Paracoccidioides brasiliensis.
PG  - 774-784
LID - 10.1093/mmy/myw135 [doi]
AB  - MicroRNAs (miRNAs) are small single stranded RNA sequences involved in 
      post-transcriptional regulation of different biological and physiological 
      processes. Paracoccidioidomycosis (PCM) is an infection caused by 
      Paracoccidioides brasiliensis, and it is a major cause of mortality due to 
      systemic mycoses in Brazil. To date, there have been few reports on the role of 
      miRNAs in the immune response against fungi, especially PCM. The objective of 
      this study was to evaluate the differential expression of miRNAs related to the 
      inflammatory response associated with pulmonary infection by P. brasiliensis. For 
      this purpose, lungs from BALB/c mice, intravenously infected with P. brasiliensis 
      (2.7x107 yeast cells/ml, n = 12) and noninfected BALB/c mice (n = 8), were 
      collected at the 28 and 56 day after infection. The lung parenchyma presented a 
      great number of yeast cells, granulomas, and edema at 28 days and a framework of 
      resolution of the inflammatory process after 56 days. The mRNAs gata-3, ror-gammat, 
      foxp3, and IL-6 were positively regulated at the moment at the 56 day, while the 
      TGF-beta1 mRNA was positively regulated at both moments. The miRNAs 126a-5p, 340-5p, 
      30b-5p, 19b-3p, 221-3p, 20a-5p, 130a-3p, and 301a-3p, 466k presented the greatest 
      increase in expression levels 28 days after infection, and the miRNAs let-7f-5p, 
      let-7a-5p, 5p-26b, let-7e-5p and 369-3p, 466k presented a greater increase in 
      levels of expression 56 days after infection. This study shows a set of 
      differentially expressed miRNAs possibly involved in the immune response in mice 
      during pulmonary infection by P. brasiliensis.
CI  - (c) The Author 2016. Published by Oxford University Press on behalf of The 
      International Society for Human and Animal Mycology. All rights reserved. For 
      permissions, please e-mail: journals.permissions@oup.com.
FAU - Turini Gonzales Marioto, Denise
AU  - Turini Gonzales Marioto D
AD  - Postgraduate Program in Experimental Pathology, Department of Pathological 
      Sciences - State University of Londrina, Londrina, PR, Brazil.
FAU - Navarro Dos Santos Ferraro, Ana Carolina
AU  - Navarro Dos Santos Ferraro AC
AD  - Postgraduate Program in Experimental Pathology, Department of Pathological 
      Sciences - State University of Londrina, Londrina, PR, Brazil.
FAU - Goulart de Andrade, Fabio
AU  - Goulart de Andrade F
AD  - Department of Histology - State University of Londrina, Londrina, PR, Brazil.
FAU - Barros Oliveira, Marilia
AU  - Barros Oliveira M
AD  - Department of Biochemistry and Molecular Biology, Federal University of Goias, 
      Goiania, GO, Brazil.
FAU - Itano, Eiko Nakagawa
AU  - Itano EN
AD  - Postgraduate Program in Experimental Pathology, Department of Pathological 
      Sciences - State University of Londrina, Londrina, PR, Brazil.
FAU - Petrofeza, Silvana
AU  - Petrofeza S
AD  - Department of Biochemistry and Molecular Biology, Federal University of Goias, 
      Goiania, GO, Brazil.
FAU - Venancio, Emerson Jose
AU  - Venancio EJ
AD  - Postgraduate Program in Experimental Pathology, Department of Pathological 
      Sciences - State University of Londrina, Londrina, PR, Brazil.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Med Mycol
JT  - Medical mycology
JID - 9815835
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Gene Expression Profiling
MH  - Lung/*pathology
MH  - Male
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/*analysis
MH  - Paracoccidioidomycosis/*pathology
OTO - NOTNLM
OT  - fungal infection
OT  - lung parenchyma
OT  - microRNA
OT  - paracoccidioidomycosis
EDAT- 2017/01/06 06:00
MHDA- 2018/05/23 06:00
CRDT- 2017/01/06 06:00
PHST- 2016/07/07 00:00 [received]
PHST- 2016/11/29 00:00 [accepted]
PHST- 2017/01/06 06:00 [pubmed]
PHST- 2018/05/23 06:00 [medline]
PHST- 2017/01/06 06:00 [entrez]
AID - myw135 [pii]
AID - 10.1093/mmy/myw135 [doi]
PST - ppublish
SO  - Med Mycol. 2017 Oct 1;55(7):774-784. doi: 10.1093/mmy/myw135.