PMID- 28053185
OWN - NLM
STAT- MEDLINE
DCOM- 20170828
LR  - 20210217
IS  - 1539-7262 (Electronic)
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 58
IP  - 3
DP  - 2017 Mar
TI  - Macrophage LTB(4) drives efficient phagocytosis of Borrelia burgdorferi via BLT1 
      or BLT2.
PG  - 494-503
LID - 10.1194/jlr.M068882 [doi]
AB  - Unresolved experimental Lyme arthritis in C3H 5-lipoxygenase (5-LOX)(-/-) mice is 
      associated with impaired macrophage phagocytosis of Borrelia burgdorferi In the 
      present study, we further investigated the effects of the 5-LOX metabolite, 
      leukotriene (LT)B(4) on phagocytosis of B. burgdorferi Bone marrow-derived 
      macrophages (BMDMs) from 5-LOX(-/-) mice were defective in the uptake and killing 
      of B. burgdorferi from the earliest stages of spirochete internalization. BMDMs 
      from mice deficient for the LTB(4) high-affinity receptor (BLT1(-/-)) were also 
      unable to efficiently phagocytose B. burgdorferi Addition of exogenous LTB(4) 
      augmented the phagocytic capability of BMDMs from both 5-LOX(-/-) and BLT1(-/-) 
      mice, suggesting that the low-affinity LTB(4) receptor, BLT2, might be involved. 
      Blocking BLT2 activity with the specific antagonist, LY255283, inhibited 
      phagocytosis in LTB(4)-stimulated BLT1(-/-) BMDMs, demonstrating a role for BLT2. 
      However, the lack of a phagocytic defect in BLT2(-/-) BMDMs suggested that this 
      was a compensatory effect. In contrast, 12(S)-hydroxyheptadeca-5Z,8E,10E-trienoic 
      acid, a natural BLT2-specific high-affinity ligand, and resolvin E1, a BLT1 
      agonist, were both unable to boost phagocytosis in BMDMs from either 5-LOX(-/-) 
      or BLT1(-/-) mice, suggesting a specific role for LTB(4) in mediating 
      phagocytosis in murine macrophages. This study demonstrates that LTB(4) promotes 
      macrophage phagocytosis of bacteria via BLT1, and that BLT2 can fulfill this role 
      in the absence of BLT1.
CI  - Copyright (c) 2017 by the American Society for Biochemistry and Molecular Biology, 
      Inc.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Department of Veterinary Pathobiology, College of Veterinary Medicine, University 
      of Missouri-Columbia, Columbia, MO 65211.
FAU - Olson, Rachel M
AU  - Olson RM
AD  - Department of Veterinary Pathobiology, College of Veterinary Medicine, University 
      of Missouri-Columbia, Columbia, MO 65211.
FAU - Brown, Charles R
AU  - Brown CR
AD  - Department of Veterinary Pathobiology, College of Veterinary Medicine, University 
      of Missouri-Columbia, Columbia, MO 65211 brownchar@missouri.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170104
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (Ltb4r1 protein, mouse)
RN  - 0 (Ltb4r2 protein, mouse)
RN  - 0 (Receptors, Leukotriene B4)
RN  - 0 (Tetrazoles)
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - EC 1.13.11.34 (Arachidonate 5-Lipoxygenase)
RN  - H037W1I5AL (LY 255283)
SB  - IM
MH  - Animals
MH  - Arachidonate 5-Lipoxygenase/*genetics/metabolism
MH  - Borrelia burgdorferi/genetics/pathogenicity
MH  - Disease Models, Animal
MH  - Humans
MH  - Leukotriene B4/administration & dosage/genetics/metabolism
MH  - Lyme Disease/*genetics/metabolism/microbiology/pathology
MH  - Macrophages/metabolism/pathology
MH  - Mice
MH  - Mice, Transgenic
MH  - Phagocytosis/genetics
MH  - Receptors, Leukotriene B4/antagonists & inhibitors/*genetics
MH  - Tetrazoles/administration & dosage
PMC - PMC5335579
OTO - NOTNLM
OT  - bacteria
OT  - bone marrow-derived macrophage
OT  - eicosanoids
OT  - immunology
OT  - leukotriene B4
OT  - leukotriene B4 receptor 1
OT  - leukotriene B4 receptor 2
OT  - leukotrienes
OT  - phagocytosis
OT  - receptors
EDAT- 2017/01/06 06:00
MHDA- 2017/08/29 06:00
PMCR- 2018/03/01
CRDT- 2017/01/06 06:00
PHST- 2016/04/28 00:00 [received]
PHST- 2016/12/15 00:00 [revised]
PHST- 2017/01/06 06:00 [pubmed]
PHST- 2017/08/29 06:00 [medline]
PHST- 2017/01/06 06:00 [entrez]
PHST- 2018/03/01 00:00 [pmc-release]
AID - S0022-2275(20)30443-0 [pii]
AID - m068882 [pii]
AID - 10.1194/jlr.M068882 [doi]
PST - ppublish
SO  - J Lipid Res. 2017 Mar;58(3):494-503. doi: 10.1194/jlr.M068882. Epub 2017 Jan 4.