PMID- 28054857
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1551-5044 (Electronic)
IS  - 0022-1554 (Linking)
VI  - 31
IP  - 1A_suppl
DP  - 1983 Jan
TI  - Rapid Analysis of Normal and Abnormal Cell Types in Human Semen and Testis 
      Biopsies by Flow Cytometry (1)(2).
PG  - 248-253
LID - 10.1177/31.1A_SUPPL.6186729 [doi]
AB  - A simple, rapid procedure is described that quantitates RNA content and DNA 
      content/chromatin condensation for each of many possible cell types and 
      differentiation levels of the cells present in human semen. A fresh semen sample 
      (1-6 hr postemission) or frozen sample (allowing samples to be accumulated and 
      sent to a laboratory) is treated with a detergent solution, stained with acridine 
      orange (AO), and measured by flow cytometry (FCM); approximately 10 minutes are 
      required to measure 5,000 cells per sample and analyze the data with computer 
      assistance. The following can be learned from a single measurement: a) the 
      percentage of each cell type in semen including, i) mature sperm, ii) immature 
      sperm precursor cells, representing all stages of development from spermatogonia 
      to mature sperm, iii) somatic cells, e.g., leukocytes; b) normality/abnormality 
      of sperm nuclear chromatin condensation. These measurements can be correlated 
      with cell types in testis biopsies identified by two-parameter FCM measurements 
      (RNA versus DNA) using AO as the fluorescent probe.
FAU - Evenson, Donald P
AU  - Evenson DP
AD  - Investigative Cytology Laboratory, Memorial Sloan-Kettering Cancer Center, New 
      York. New York (OA 82-293P4).
FAU - Melamed, Myron R
AU  - Melamed MR
AD  - Investigative Cytology Laboratory, Memorial Sloan-Kettering Cancer Center, New 
      York. New York (OA 82-293P4).
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
OTO - NOTNLM
OT  - Altered spermiogenesis
OT  - Cancer
OT  - Chemicals
OT  - Chemotherapy
OT  - Chromatin structure
OT  - Flow cytometry.
OT  - Semen cells
OT  - Sperm
OT  - Testis
EDAT- 1983/01/01 00:00
MHDA- 1983/01/01 00:01
CRDT- 2017/01/06 06:00
PHST- 2017/01/06 06:00 [entrez]
PHST- 1983/01/01 00:00 [pubmed]
PHST- 1983/01/01 00:01 [medline]
AID - 10.1177/31.1A_SUPPL.6186729 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1983 Jan;31(1A_suppl):248-253. doi: 
      10.1177/31.1A_SUPPL.6186729.