PMID- 28055115
OWN - NLM
STAT- MEDLINE
DCOM- 20171002
LR  - 20200108
IS  - 1934-9300 (Electronic)
IS  - 1934-9297 (Linking)
VI  - 79
DP  - 2017 Jan 5
TI  - Measurement of T-Cell Telomere Length Using Amplified-Signal FISH Staining and 
      Flow Cytometry.
PG  - 7.47.1-7.47.10
LID - 10.1002/cpcy.11 [doi]
AB  - Exposure to pathogen-associated molecular patterns (PAMPS), damage-associated 
      molecular patterns (DAMPS), and physiologically challenging stimuli either 
      positively or negatively affect leukocyte maturity. Cellular maturity has 
      implications for the effectiveness of host response to bacterial or viral 
      infection and/or tissue injury. Thus, the ability to accurately assess cellular 
      maturity and health is important to fully understand immune status and function. 
      The most common technique for measuring cellular maturity is to measure telomere 
      length; however, existing techniques are not optimized for single-cell 
      measurements using flow cytometry. Specifically, existing methods used to measure 
      telomere length are PCR-based, making it difficult for a researcher to measure 
      maturity within specific leukocyte subsets (e.g., T cells). In this report, we 
      describe a new approach for the measurement of telomere length within individual 
      T cells using an amplified fluorescence in situ hybridization (FISH) technique 
      (PrimeFlow RNA Assay). The unique aspect of this technique is that it amplifies 
      the fluorescent signal rather than the target up to 3000-fold, resulting in the 
      detection of as few as 1 copy of the target nucleic acid. While the current 
      technique focuses on human T cells, this method can be broadly applied to a 
      variety of cell types and disease models. (c) 2017 by John Wiley & Sons, Inc.
CI  - Copyright (c) 2017 John Wiley & Sons, Inc.
FAU - Henning, Andrea L
AU  - Henning AL
AD  - Applied Physiology Laboratory, University of North Texas, Denton, Texas.
AD  - Department of Biological Sciences, University of North Texas, Denton, Texas.
FAU - Levitt, Danielle E
AU  - Levitt DE
AD  - Applied Physiology Laboratory, University of North Texas, Denton, Texas.
AD  - Department of Biological Sciences, University of North Texas, Denton, Texas.
FAU - Vingren, Jakob L
AU  - Vingren JL
AD  - Applied Physiology Laboratory, University of North Texas, Denton, Texas.
AD  - Department of Biological Sciences, University of North Texas, Denton, Texas.
FAU - McFarlin, Brian K
AU  - McFarlin BK
AD  - Applied Physiology Laboratory, University of North Texas, Denton, Texas.
AD  - Department of Biological Sciences, University of North Texas, Denton, Texas.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170105
PL  - United States
TA  - Curr Protoc Cytom
JT  - Current protocols in cytometry
JID - 100899351
SB  - IM
MH  - Cell Membrane/metabolism
MH  - Flow Cytometry/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Staining and Labeling
MH  - T-Lymphocytes/*metabolism
MH  - Telomere/*metabolism
OTO - NOTNLM
OT  - cell health
OT  - cellular aging
OT  - disease risk
OT  - senescence
EDAT- 2017/01/06 06:00
MHDA- 2017/10/03 06:00
CRDT- 2017/01/06 06:00
PHST- 2017/01/06 06:00 [entrez]
PHST- 2017/01/06 06:00 [pubmed]
PHST- 2017/10/03 06:00 [medline]
AID - 10.1002/cpcy.11 [doi]
PST - epublish
SO  - Curr Protoc Cytom. 2017 Jan 5;79:7.47.1-7.47.10. doi: 10.1002/cpcy.11.