PMID- 28056323
OWN - NLM
STAT- MEDLINE
DCOM- 20170605
LR  - 20181202
IS  - 0578-1426 (Print)
IS  - 0578-1426 (Linking)
VI  - 56
IP  - 1
DP  - 2017 Jan 1
TI  - [The role of neuroglobin in oxygen-glucose deprivation and reoxygenation-induced 
      mitochondrial depolarization and reactive oxygen species production in SH-SY5Y 
      cells].
PG  - 44-48
LID - 10.3760/cma.j.issn.0578-1426.2017.01.011 [doi]
AB  - Objective: To investigate the role of neuroglobin (NGB) in oxygen-glucose 
      deprivation and reoxygenation (OGD/R) induced mitochondrial depolarization and 
      reactive oxygen species (ROS)production in a human neuroblastoma cell line 
      (SH-SY5Y). Methods: SH-SY5Y cells were transfected with lentivirus to establish a 
      stable cell line of NGB knockdown (KD). After treated with OGD/R, cells were 
      collected at different time points to analyze NGB mRNA and protein levels. 
      Furthermore, cells were stained with JC-1 and DCFH-DA to evaluate mitochondrial 
      depolarization and ROS production by inverted fluorescence microscope. Also, to 
      determine the neurotoxicity, we measured the lactate dehydrogenase(LDH)level in 
      the cell culture medium. Results: After the treatment of OGD/R, the NGB mRNA and 
      protein started to elevate and peak at 4 h and 8 h (2.04+/-0.35 fold, 1.69+/-0.18 
      fold). Compared with the vector group, NGB KD group had much more mitochondrial 
      depolarization [JC-1 red/green (1.10+/-0.10) vs (1.46+/-0.11), P<0.05] and ROS 
      production [DCFH-DA fluorescence (36.30+/-5.32) vs (16.26+/-2.97), P<0.05]. 
      Furthermore, NGB KD groups had a higher level of LDH release [(63.42+/-6.14)%vs 
      (49.65+/-5.09)%, P<0.05]. Conclusions: NGB plays an important role in the 
      homeostasis of mitochondria. Knockdown of NGB results in increased mitochondrial 
      depolarization, ROS production and neurotoxicity under hypoxia circumstances.
FAU - Deng, S Y
AU  - Deng SY
AD  - Department of Critical Care Medicine, Xiangya Hospital, Central South University, 
      Changsha 410008, China.
FAU - Ai, Y H
AU  - Ai YH
FAU - Zhang, L N
AU  - Zhang LN
FAU - Wu, L
AU  - Wu L
FAU - Chen, C X
AU  - Chen CX
FAU - Wang, Y M
AU  - Wang YM
FAU - Liu, Z Y
AU  - Liu ZY
FAU - Huang, L
AU  - Huang L
FAU - Peng, Q Y
AU  - Peng QY
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Nei Ke Za Zhi
JT  - Zhonghua nei ke za zhi
JID - 16210490R
RN  - 0 (Fluoresceins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuroglobin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 2044-85-1 (diacetyldichlorofluorescein)
RN  - 9004-22-2 (Globins)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Cells, Cultured
MH  - Fluoresceins
MH  - Globins/genetics/metabolism/*physiology
MH  - Glucose/*deficiency/metabolism/*pharmacology
MH  - Homeostasis/*drug effects
MH  - Humans
MH  - Hypoxia/*pathology
MH  - Nerve Tissue Proteins/genetics/metabolism/*physiology
MH  - Neuroglobin
MH  - Oxygen/metabolism/pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Transfection
EDAT- 2017/01/06 06:00
MHDA- 2017/06/06 06:00
CRDT- 2017/01/06 06:00
PHST- 2017/01/06 06:00 [entrez]
PHST- 2017/01/06 06:00 [pubmed]
PHST- 2017/06/06 06:00 [medline]
AID - 10.3760/cma.j.issn.0578-1426.2017.01.011 [doi]
PST - ppublish
SO  - Zhonghua Nei Ke Za Zhi. 2017 Jan 1;56(1):44-48. doi: 
      10.3760/cma.j.issn.0578-1426.2017.01.011.