PMID- 28057764
OWN - NLM
STAT- MEDLINE
DCOM- 20170919
LR  - 20190402
IS  - 1939-4586 (Electronic)
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 28
IP  - 5
DP  - 2017 Mar 1
TI  - Regulation of yeast ESCRT-III membrane scission activity by the Doa4 ubiquitin 
      hydrolase.
PG  - 661-672
LID - 10.1091/mbc.E16-11-0761 [doi]
AB  - ESCRT-III executes membrane scission during the budding of intralumenal vesicles 
      (ILVs) at endosomes. The scission mechanism is unknown but appears to be linked 
      to the cycle of assembly and disassembly of ESCRT-III complexes at membranes. 
      Regulating this cycle is therefore expected to be important for determining the 
      timing of ESCRT-III-mediated membrane scission. We show that in Saccharomyces 
      cerevisiae, ESCRT-III complexes are stabilized and ILV membrane scission is 
      delayed by Doa4, which is the ubiquitin hydrolase that deubiquitinates 
      transmembrane proteins sorted as cargoes into ILVs. These results suggest a 
      mechanism to delay ILV budding while cargoes undergo deubiquitination. We further 
      show that deubiquitination of ILV cargoes is inhibited via Doa4 binding to Vps20, 
      which is the subunit of ESCRT-III that initiates assembly of the complex. Current 
      models suggest that ESCRT-III complexes surround ubiquitinated cargoes to trap 
      them at the site of ILV budding while the cargoes undergo deubiquitination. Thus 
      our results also propose a mechanism to prevent the onset of ILV cargo 
      deubiquitination at the initiation of ESCRT-III complex assembly.
CI  - (c) 2017 Johnson et al. This article is distributed by The American Society for 
      Cell Biology under license from the author(s). Two months after publication it is 
      available to the public under an Attribution-Noncommercial-Share Alike 3.0 
      Unported Creative Commons License 
      (http://creativecommons.org/licenses/by-nc-sa/3.0).
FAU - Johnson, Natalie
AU  - Johnson N
AD  - Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, 
      CO 80309.
FAU - West, Matt
AU  - West M
AD  - Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, 
      CO 80309.
FAU - Odorizzi, Greg
AU  - Odorizzi G
AD  - Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, 
      CO 80309 odorizzi@colorado.edu.
LA  - eng
GR  - R01 GM111335/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20170105
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (DOA4 protein, S cerevisiae)
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Ubiquitin)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
SB  - IM
MH  - Cell Division/physiology
MH  - Endopeptidases/*metabolism
MH  - Endosomal Sorting Complexes Required for Transport/*metabolism
MH  - Endosomes/metabolism
MH  - Intracellular Membranes/metabolism
MH  - Multivesicular Bodies/metabolism
MH  - Protein Binding
MH  - Protein Transport
MH  - Saccharomyces cerevisiae/cytology/metabolism
MH  - Saccharomyces cerevisiae Proteins/*metabolism
MH  - Ubiquitin/metabolism
MH  - Ubiquitin Thiolesterase/*metabolism
PMC - PMC5328624
EDAT- 2017/01/07 06:00
MHDA- 2017/09/20 06:00
PMCR- 2017/05/16
CRDT- 2017/01/07 06:00
PHST- 2016/11/04 00:00 [received]
PHST- 2016/12/15 00:00 [revised]
PHST- 2016/12/30 00:00 [accepted]
PHST- 2017/01/07 06:00 [pubmed]
PHST- 2017/09/20 06:00 [medline]
PHST- 2017/01/07 06:00 [entrez]
PHST- 2017/05/16 00:00 [pmc-release]
AID - mbc.E16-11-0761 [pii]
AID - E16-11-0761 [pii]
AID - 10.1091/mbc.E16-11-0761 [doi]
PST - ppublish
SO  - Mol Biol Cell. 2017 Mar 1;28(5):661-672. doi: 10.1091/mbc.E16-11-0761. Epub 2017 
      Jan 5.