PMID- 28058088
OWN - NLM
STAT- MEDLINE
DCOM- 20170313
LR  - 20181113
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2016
DP  - 2016
TI  - Long-Term Alteration of Reactive Oxygen Species Led to Multidrug Resistance in 
      MCF-7 Cells.
PG  - 7053451
LID - 10.1155/2016/7053451 [doi]
LID - 7053451
AB  - Reactive oxygen species (ROS) play an important role in multidrug resistance 
      (MDR). This study aimed to investigate the effects of long-term ROS alteration on 
      MDR in MCF-7 cells and to explore its underlying mechanism. Our study showed both 
      long-term treatments of H(2)O(2) and glutathione (GSH) led to MDR with suppressed 
      iROS levels in MCF-7 cells. Moreover, the MDR cells induced by 0.1 muM H(2)O(2) 
      treatment for 20 weeks (MCF-7/ROS cells) had a higher viability and proliferative 
      ability than the control MCF-7 cells. MCF-7/ROS cells also showed higher activity 
      or content of intracellular antioxidants like glutathione peroxidase (GPx), GSH, 
      superoxide dismutase (SOD), and catalase (CAT). Importantly, MCF-7/ROS cells were 
      characterized by overexpression of MDR-related protein 1 (MRP1) and 
      P-glycoprotein (P-gp), as well as their regulators NF-E2-related factor 2 (Nrf2), 
      hypoxia-inducible factor 1 (HIF-1alpha), and the activation of PI3K/Akt pathway in 
      upstream. Moreover, several typical MDR mediators, including glutathione 
      S-transferase-pi (GST-pi) and c-Myc and Protein Kinase Calpha (PKCalpha), were also found 
      to be upregulated in MCF-7/ROS cells. Collectively, our results suggest that ROS 
      may be critical in the generation of MDR, which may provide new insights into 
      understanding of mechanisms of MDR.
FAU - Cen, Juan
AU  - Cen J
AD  - Key Laboratory of Natural Medicine and Immune Engineering, Henan University, 
      Kaifeng, China.
FAU - Zhang, Li
AU  - Zhang L
AD  - Key Laboratory of Natural Medicine and Immune Engineering, Henan University, 
      Kaifeng, China.
FAU - Liu, Fangfang
AU  - Liu F
AD  - Key Laboratory of Natural Medicine and Immune Engineering, Henan University, 
      Kaifeng, China.
FAU - Zhang, Feng
AU  - Zhang F
AUID- ORCID: 0000-0001-7414-0450
AD  - School of Pharmacy, Henan University, Kaifeng, China.
FAU - Ji, Bian-Sheng
AU  - Ji BS
AUID- ORCID: 0000-0001-9203-9663
AD  - Key Laboratory of Natural Medicine and Immune Engineering, Henan University, 
      Kaifeng, China.
LA  - eng
PT  - Journal Article
DEP - 20161212
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Antioxidants)
RN  - 0 (Reactive Oxygen Species)
RN  - 1N3CZ14C5O (Rhodamine 123)
RN  - 80168379AG (Doxorubicin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Antioxidants/metabolism
MH  - Biological Transport/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Doxorubicin/pharmacology
MH  - Drug Resistance, Multiple/*drug effects
MH  - Drug Resistance, Neoplasm/*drug effects
MH  - Glutathione/pharmacology
MH  - Humans
MH  - Hydrogen Peroxide/pharmacology
MH  - Intracellular Space/metabolism
MH  - MCF-7 Cells
MH  - Models, Biological
MH  - Reactive Oxygen Species/*pharmacology
MH  - Rhodamine 123/pharmacology
MH  - Signal Transduction/drug effects
MH  - Time Factors
MH  - Up-Regulation/drug effects
PMC - PMC5183793
COIS- The authors declare that there is no conflict of interests regarding the 
      publication of this paper.
EDAT- 2017/01/07 06:00
MHDA- 2017/03/14 06:00
PMCR- 2016/12/12
CRDT- 2017/01/07 06:00
PHST- 2016/08/18 00:00 [received]
PHST- 2016/10/25 00:00 [revised]
PHST- 2016/11/06 00:00 [accepted]
PHST- 2017/01/07 06:00 [entrez]
PHST- 2017/01/07 06:00 [pubmed]
PHST- 2017/03/14 06:00 [medline]
PHST- 2016/12/12 00:00 [pmc-release]
AID - 10.1155/2016/7053451 [doi]
PST - ppublish
SO  - Oxid Med Cell Longev. 2016;2016:7053451. doi: 10.1155/2016/7053451. Epub 2016 Dec 
      12.