PMID- 28059075
OWN - NLM
STAT- MEDLINE
DCOM- 20181018
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
DP  - 2017 Jan 6
TI  - Deficiency of the eIF4E isoform nCBP limits the cell-to-cell movement of a plant 
      virus encoding triple-gene-block proteins in Arabidopsis thaliana.
PG  - 39678
LID - 10.1038/srep39678 [doi]
LID - 39678
AB  - One of the important antiviral genetic strategies used in crop breeding is 
      recessive resistance. Two eukaryotic translation initiation factor 4E family 
      genes, eIF4E and eIFiso4E, are the most common recessive resistance genes whose 
      absence inhibits infection by plant viruses in Potyviridae, Carmovirus, and 
      Cucumovirus. Here, we show that another eIF4E family gene, nCBP, acts as a novel 
      recessive resistance gene in Arabidopsis thaliana toward plant viruses in Alpha- 
      and Betaflexiviridae. We found that infection by Plantago asiatica mosaic virus 
      (PlAMV), a potexvirus, was delayed in ncbp mutants of A. thaliana. Virus 
      replication efficiency did not differ between an ncbp mutant and a wild type 
      plant in single cells, but viral cell-to-cell movement was significantly delayed 
      in the ncbp mutant. Furthermore, the accumulation of triple-gene-block protein 2 
      (TGB2) and TGB3, the movement proteins of potexviruses, decreased in the ncbp 
      mutant. Inoculation experiments with several viruses showed that the accumulation 
      of viruses encoding TGBs in their genomes decreased in the ncbp mutant. These 
      results indicate that nCBP is a novel member of the eIF4E family recessive 
      resistance genes whose loss impairs viral cell-to-cell movement by inhibiting the 
      efficient accumulation of TGB2 and TGB3.
FAU - Keima, Takuya
AU  - Keima T
AD  - Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 
      Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Hagiwara-Komoda, Yuka
AU  - Hagiwara-Komoda Y
AD  - Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 
      Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Hashimoto, Masayoshi
AU  - Hashimoto M
AD  - Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 
      Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Neriya, Yutaro
AU  - Neriya Y
AD  - Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 
      Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Koinuma, Hiroaki
AU  - Koinuma H
AD  - Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 
      Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Iwabuchi, Nozomu
AU  - Iwabuchi N
AD  - Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 
      Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Nishida, Shuko
AU  - Nishida S
AD  - Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 
      Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Yamaji, Yasuyuki
AU  - Yamaji Y
AD  - Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 
      Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Namba, Shigetou
AU  - Namba S
AD  - Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 
      Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170106
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Eukaryotic Initiation Factor-4E)
RN  - 0 (Plant Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Viral Proteins)
RN  - Plantago asiatica mosaic virus
SB  - IM
MH  - Arabidopsis/*genetics/*virology
MH  - Cell Movement
MH  - Disease Resistance
MH  - Eukaryotic Initiation Factor-4E/*genetics
MH  - Mutation
MH  - Plant Proteins/*genetics
MH  - Potexvirus/*pathogenicity
MH  - Protein Isoforms/genetics
MH  - Viral Proteins/metabolism
PMC - PMC5216350
EDAT- 2017/01/07 06:00
MHDA- 2018/10/20 06:00
PMCR- 2017/01/06
CRDT- 2017/01/07 06:00
PHST- 2016/10/06 00:00 [received]
PHST- 2016/11/25 00:00 [accepted]
PHST- 2017/01/07 06:00 [entrez]
PHST- 2017/01/07 06:00 [pubmed]
PHST- 2018/10/20 06:00 [medline]
PHST- 2017/01/06 00:00 [pmc-release]
AID - srep39678 [pii]
AID - 10.1038/srep39678 [doi]
PST - epublish
SO  - Sci Rep. 2017 Jan 6;7:39678. doi: 10.1038/srep39678.