PMID- 28059125
OWN - NLM
STAT- MEDLINE
DCOM- 20181029
LR  - 20211204
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
DP  - 2017 Jan 6
TI  - Neuronal apoptosis inhibitory protein (NAIP) localizes to the cytokinetic 
      machinery during cell division.
PG  - 39981
LID - 10.1038/srep39981 [doi]
LID - 39981
AB  - The neuronal apoptosis inhibitory protein (NAIP) is a constituent of the 
      inflammasome and a key component of the innate immune system. Here we use 
      immunofluorescence to position NAIP within the cytokinetic apparatus, contiguous 
      to chromosomal passenger complex (CPC), Centralspindlin, PRC1 and KIF4A. During 
      metaphase, NAIP accumulates in the mitotic spindle poles and is shown in spindle 
      microtubules; in anaphase NAIP is detected in the middle of the central spindle. 
      At the end of cytokinesis, NAIP is localized in the outlying region of the stem 
      body, the center of the intercellular bridge formed between daughter cells prior 
      to cellular abscission. We also describe the sustained presence of NAIP mRNA and 
      protein throughout the cell cycle with a significant increase observed in the 
      G2/M phase. Consistent with a role for NAIP in cytokinesis, NAIP overexpression 
      in HeLa cells promotes the acquisition of a multinuclear phenotype. Conversely, 
      NAIP siRNA gene silencing results in an apoptotic lethal phenotype. Our confocal 
      and super resolution stimulated-emission-depletion (STED) examination of 
      mammalian cell cytokinesis demonstrate a potential new role for NAIP in addition 
      to anti-apoptotic and innate immunology functions.
FAU - Abadia-Molina, Francisco
AU  - Abadia-Molina F
AD  - Department of Cell Biology, University of Granada, Granada 18071, Spain.
AD  - Biomedical Research Centre, University of Granada, Granada 18100, Spain.
FAU - Moron-Calvente, Virginia
AU  - Moron-Calvente V
AD  - Department of Cell Biology, University of Granada, Granada 18071, Spain.
AD  - Biomedical Research Centre, University of Granada, Granada 18100, Spain.
FAU - Baird, Stephen D
AU  - Baird SD
AD  - Children's Hospital of Eastern Ontario Research Institute, University of Ottawa, 
      Ottawa ON K1H 8L1, Canada.
FAU - Shamim, Fahad
AU  - Shamim F
AD  - Children's Hospital of Eastern Ontario Research Institute, University of Ottawa, 
      Ottawa ON K1H 8L1, Canada.
FAU - Martin, Francisco
AU  - Martin F
AD  - Department of Human DNA Variability, GENYO, Centre for Genomics and Oncological 
      Research, Pfizer, University of Granada, Andalusian Regional Government, Granada, 
      18016 Spain.
FAU - MacKenzie, Alex
AU  - MacKenzie A
AD  - Children's Hospital of Eastern Ontario Research Institute, University of Ottawa, 
      Ottawa ON K1H 8L1, Canada.
AD  - Department of Pediatrics, University of Ottawa, Ottawa ON K1H 8L1, Canada.
LA  - eng
GR  - CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170106
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (NAIP protein, human)
RN  - 0 (Neuronal Apoptosis-Inhibitory Protein)
RN  - 0 (PRC1 protein, human)
RN  - EC 3.6.1.- (KIF4A protein, human)
RN  - EC 3.6.4.4 (Kinesins)
SB  - IM
MH  - Cell Cycle Proteins/metabolism
MH  - Cell Survival
MH  - *Cytokinesis
MH  - HeLa Cells
MH  - Humans
MH  - Kinesins/metabolism
MH  - Microscopy, Confocal
MH  - Mitosis
MH  - Neuronal Apoptosis-Inhibitory Protein/*genetics/*metabolism
MH  - Phenotype
MH  - Spindle Apparatus/*metabolism
MH  - Spindle Poles/metabolism
MH  - Up-Regulation
PMC - PMC5216396
EDAT- 2017/01/07 06:00
MHDA- 2018/10/30 06:00
PMCR- 2017/01/06
CRDT- 2017/01/07 06:00
PHST- 2016/01/14 00:00 [received]
PHST- 2016/11/30 00:00 [accepted]
PHST- 2017/01/07 06:00 [entrez]
PHST- 2017/01/07 06:00 [pubmed]
PHST- 2018/10/30 06:00 [medline]
PHST- 2017/01/06 00:00 [pmc-release]
AID - srep39981 [pii]
AID - 10.1038/srep39981 [doi]
PST - epublish
SO  - Sci Rep. 2017 Jan 6;7:39981. doi: 10.1038/srep39981.