PMID- 28060008
OWN - NLM
STAT- MEDLINE
DCOM- 20180102
LR  - 20181027
IS  - 1473-5571 (Electronic)
IS  - 0269-9370 (Linking)
VI  - 31
IP  - 5
DP  - 2017 Mar 13
TI  - Acute HIV-1 infection is associated with increased plasma levels of heme 
      oxygenase-1 and presence of heme oxygenase-1-specific regulatory T cells.
PG  - 635-641
LID - 10.1097/QAD.0000000000001390 [doi]
AB  - OBJECTIVE: Heme oxygenase-1 (HO-1) is an inducible stress response protein with 
      potent anti-inflammatory activity and recent data suggest a potentially 
      beneficial role in HIV pathogenesis. We investigated the impact of HO-1 and a 
      novel subset of HO-1-specific CD8 regulatory T cells on virus-specific T-cell 
      immunity in HIV-1-infected individuals. METHODS: HO-1 protein levels were 
      quantified in plasma from individuals at different stages of HIV-1 disease and 
      longitudinally following primary HIV infection. HO-1-specific CD8 T cells were 
      investigated by flow cytometry using human leukocyte antigen (HLA) class I 
      pentamers. Flow-sorted HO-1-specific CD8 T cells were cultured and tested for 
      suppressive activity on HIV-1-specific cytotoxic T-cell clones clones. HO-1 gene 
      expression was determined in sorted peripheral blood mononuclear cell (PBMC) 
      subsets from individuals with acute HIV-1 infection. RESULTS: HO-1 plasma levels 
      were significantly increased in HIV-1 infection, with the highest levels in 
      individuals with acute HIV-1 infection, and gradually declined over time. The 
      frequency of CD8 T cells specific for HO-1 was elevated in study participants 
      with primary HIV-1 infection and flow-sorted HO-1-specific CD8 T cells were 
      capable of suppressing HIV-1-specific lysis of cytotoxic T-cell clones clones. 
      HO-1 gene expression was upregulated in multiple immune cell subsets during acute 
      HIV-1 infection and HO-1 overexpression modulated anti-HIV immunity in vitro. 
      CONCLUSION: Our data suggest that HO-1 is induced during acute HIV-1 infection, 
      likely mediating anti-inflammatory effects and driving expansion of HO-1-specific 
      CD8 regulatory T cells capable of suppressing HIV-1-specific immune responses in 
      vitro. The investigation of HO-1 and the novel CD8 regulatory cell type described 
      here provide further insight into immune regulation in HIV-1 infection and may 
      hold potential for future immunotherapeutic intervention.
FAU - Angin, Mathieu
AU  - Angin M
AD  - aRagon Institute of MGH, MIT and Harvard, Boston, Massachusetts, USA bUniversity 
      of Munster, Munster, North Rhine-Westphalia cDepartment of Viral Immunology, 
      Leibniz Institute for Experimental Virology, Hamburg, Germany dDivision of 
      Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA 
      eI. Department of Medicine, University Medical Center Hamburg-Eppendorf fGerman 
      Center for Infection Research (DZIF), Standort Hamburg-Luebeck-Borstel, Hamburg, 
      Germany.
FAU - Fathi, Anahita
AU  - Fathi A
FAU - King, Melanie
AU  - King M
FAU - Ledoux, Mary B
AU  - Ledoux MB
FAU - Piechocka-Trocha, Alicja
AU  - Piechocka-Trocha A
FAU - Altfeld, Marcus
AU  - Altfeld M
FAU - Addo, Marylyn M
AU  - Addo MM
LA  - eng
PT  - Journal Article
PL  - England
TA  - AIDS
JT  - AIDS (London, England)
JID - 8710219
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
SB  - IM
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cells, Cultured
MH  - Flow Cytometry
MH  - HIV Infections/*immunology/virology
MH  - HIV-1/*immunology
MH  - Heme Oxygenase-1/*blood
MH  - Humans
MH  - Immunophenotyping
MH  - Longitudinal Studies
MH  - Plasma/*enzymology
MH  - T-Lymphocytes, Regulatory/*immunology
EDAT- 2017/01/07 06:00
MHDA- 2018/01/03 06:00
CRDT- 2017/01/07 06:00
PHST- 2017/01/07 06:00 [pubmed]
PHST- 2018/01/03 06:00 [medline]
PHST- 2017/01/07 06:00 [entrez]
AID - 10.1097/QAD.0000000000001390 [doi]
PST - ppublish
SO  - AIDS. 2017 Mar 13;31(5):635-641. doi: 10.1097/QAD.0000000000001390.