PMID- 28060279
OWN - NLM
STAT- MEDLINE
DCOM- 20170814
LR  - 20181227
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 118
DP  - 2016 Dec 26
TI  - Quantitative 3D In Silico Modeling (q3DISM) of Cerebral Amyloid-beta Phagocytosis 
      in Rodent Models of Alzheimer's Disease.
LID - 10.3791/54868 [doi]
LID - 54868
AB  - Neuroinflammation is now recognized as a major etiological factor in 
      neurodegenerative disease. Mononuclear phagocytes are innate immune cells 
      responsible for phagocytosis and clearance of debris and detritus. These cells 
      include CNS-resident macrophages known as microglia, and mononuclear phagocytes 
      infiltrating from the periphery. Light microscopy has generally been used to 
      visualize phagocytosis in rodent or human brain specimens. However, qualitative 
      methods have not provided definitive evidence of in vivo phagocytosis. Here, we 
      describe quantitative 3D in silico modeling (q3DISM), a robust method allowing 
      for true 3D quantitation of amyloid-beta (Abeta) phagocytosis by mononuclear phagocytes 
      in rodent Alzheimer's Disease (AD) models. The method involves fluorescently 
      visualizing Abeta encapsulated within phagolysosomes in rodent brain sections. Large 
      z-dimensional confocal datasets are then 3D reconstructed for quantitation of Abeta 
      spatially colocalized within the phagolysosome. We demonstrate the successful 
      application of q3DISM to mouse and rat brains, but this methodology can be 
      extended to virtually any phagocytic event in any tissue.
FAU - Guillot-Sestier, Marie-Victoire
AU  - Guillot-Sestier MV
AD  - Zilkha Neurogenetic Institute, University of Southern California (USC).
FAU - Weitz, Tara M
AU  - Weitz TM
AD  - Zilkha Neurogenetic Institute, University of Southern California (USC).
FAU - Town, Terrence
AU  - Town T
AD  - Zilkha Neurogenetic Institute, University of Southern California (USC); 
      ttown@usc.edu.
LA  - eng
GR  - R01 NS076794/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Video-Audio Media
DEP - 20161226
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Amyloid beta-Peptides)
SB  - IM
MH  - Alzheimer Disease/*physiopathology
MH  - *Amyloid beta-Peptides
MH  - Animals
MH  - Disease Models, Animal
MH  - Humans
MH  - Imaging, Three-Dimensional/*methods
MH  - Mice
MH  - Microglia/*cytology
MH  - Models, Biological
MH  - Phagocytes/*cytology
MH  - *Phagocytosis
MH  - Rats
PMC - PMC5226459
EDAT- 2017/01/07 06:00
MHDA- 2017/08/15 06:00
PMCR- 2018/12/26
CRDT- 2017/01/07 06:00
PHST- 2017/01/07 06:00 [entrez]
PHST- 2017/01/07 06:00 [pubmed]
PHST- 2017/08/15 06:00 [medline]
PHST- 2018/12/26 00:00 [pmc-release]
AID - 54868 [pii]
AID - 10.3791/54868 [doi]
PST - epublish
SO  - J Vis Exp. 2016 Dec 26;(118):54868. doi: 10.3791/54868.