PMID- 28062126
OWN - NLM
STAT- MEDLINE
DCOM- 20171208
LR  - 20180103
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 35
IP  - 5
DP  - 2017 Feb 1
TI  - Cross reactivity of immune responses to porcine reproductive and respiratory 
      syndrome virus infection.
PG  - 782-788
LID - S0264-410X(16)31265-8 [pii]
LID - 10.1016/j.vaccine.2016.12.040 [doi]
AB  - Because porcine reproductive and respiratory syndrome virus (PRRSV) exhibits 
      extensive genetic variation among field isolates, characterizing the extent of 
      cross reactivity of immune responses, and most importantly cell-mediated immunity 
      (CMI), could help in the development of broadly cross-protective vaccines. We 
      infected 12 PRRSV-naive pigs with PRRSV strain FL12 and determined the number of 
      interferon (IFN)-gamma secreting cells (SC) by ELISpot assay using ten type 2 and one 
      type 1 PRRSV isolates as recall antigens. The number of IFN-gamma SC was extremely 
      variable among animals, and with exceptions, late to appear. Cross reactivity of 
      IFN-gamma SC among type 2 isolates was broad, and we found no evidence of an 
      association between increased genetic distance among isolates and the intensity 
      of the CMI response. Comparable to IFN-gamma SC, total antibodies evaluated by 
      indirect immunofluorescence assay (IFA) were cross reactive, however, 
      neutralizing antibody titers could only be detected against the strain used for 
      infection. Finally, we observed a moderate association between homologous IFN-gamma 
      SC and neutralizing antibodies.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Correas, Ignacio
AU  - Correas I
AD  - School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, United States; Nebraska Center for Virology, University of 
      Nebraska-Lincoln, United States.
FAU - Osorio, Fernando A
AU  - Osorio FA
AD  - School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, United States; Nebraska Center for Virology, University of 
      Nebraska-Lincoln, United States.
FAU - Steffen, David
AU  - Steffen D
AD  - School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, United States.
FAU - Pattnaik, Asit K
AU  - Pattnaik AK
AD  - School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, United States; Nebraska Center for Virology, University of 
      Nebraska-Lincoln, United States.
FAU - Vu, Hiep L X
AU  - Vu HLX
AD  - School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, United States; Nebraska Center for Virology, University of 
      Nebraska-Lincoln, United States. Electronic address: hiepvu@unl.edu.
LA  - eng
GR  - P30 GM103509/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170103
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/*biosynthesis
MH  - Antibodies, Viral/*biosynthesis
MH  - Cross Reactions
MH  - Genetic Variation
MH  - Genotype
MH  - Immunity, Cellular/*genetics
MH  - Interferon-gamma/biosynthesis/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/genetics/growth & development/*immunology
MH  - Swine
MH  - T-Lymphocytes/*immunology/virology
OTO - NOTNLM
OT  - Cell-mediated immunity
OT  - Heterologous immunity
OT  - Interferon-gamma
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - T cell cross reactivity
EDAT- 2017/01/08 06:00
MHDA- 2017/12/09 06:00
CRDT- 2017/01/08 06:00
PHST- 2016/10/26 00:00 [received]
PHST- 2016/12/16 00:00 [revised]
PHST- 2016/12/18 00:00 [accepted]
PHST- 2017/01/08 06:00 [pubmed]
PHST- 2017/12/09 06:00 [medline]
PHST- 2017/01/08 06:00 [entrez]
AID - S0264-410X(16)31265-8 [pii]
AID - 10.1016/j.vaccine.2016.12.040 [doi]
PST - ppublish
SO  - Vaccine. 2017 Feb 1;35(5):782-788. doi: 10.1016/j.vaccine.2016.12.040. Epub 2017 
      Jan 3.