PMID- 28063705
OWN - NLM
STAT- MEDLINE
DCOM- 20171208
LR  - 20171213
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 35
IP  - 5
DP  - 2017 Feb 1
TI  - H9N2 avian influenza virus enhances the immune responses of BMDCs by 
      down-regulating miR29c.
PG  - 729-737
LID - S0264-410X(16)31287-7 [pii]
LID - 10.1016/j.vaccine.2016.12.054 [doi]
AB  - Avian influenza virus (AIV) of the subtypes H9 and N2 is well recognised and 
      caused outbreaks-due to its high genetic variability and high rate of 
      recombination with other influenza virus subtypes. The pathogenicity of H9N2 AIV 
      depends on the host immune response. Dendritic cells (DCs) are major antigen 
      presenting cells that can significantly inhibit H9N2 AIV replication. MicroRNAs 
      (miRNAs) influence the ability of DCs to present antigens, as well as the ability 
      of AIVs to infect host cells and replicate. Here, we studied the molecular 
      mechanism underlying the miRNA-mediated regulation of immune function of mouse 
      DCs. We first screened for and verified the induction of miRNAs in DCs after H9N2 
      AIVstimulation. We also constructed miR29c, miR339 and miR222 over-expression 
      vector and showed that only the induction of miR29c lead to a hugely increased 
      expression of surface marker MHCII and CD40. Whilst the inhibition of miR29c, 
      miR339 and miR222 in mouse DCs would repressed the expression of DCs surface 
      markers. Moreover, we found that miR29c stimulation not only up-regulate MHCII 
      and CD40, but also enhance the ability of DCs to activate lymphocytes and secrete 
      cytokines IL-6 or TNF-a. Furthermore, we found that Tarbp1 and Rfx7 were targeted 
      and repressed by miR29c. Finally, we revealed that the inhibition of miR29c 
      marvelously accelerated virus replication. Together, our data shed new light on 
      the roles and mechanisms of miR29c in regulating DC function and suggest new 
      strategies for combating AIVs.
CI  - Copyright (c) 2016. Published by Elsevier Ltd.
FAU - Lin, Jian
AU  - Lin J
AD  - College of Life Science, Nanjing Agricultural University, Weigang 1, Nanjing, 
      Jiangsu 210095, PR China. Electronic address: linjian@njau.edu.cn.
FAU - Xia, Jing
AU  - Xia J
AD  - College of Life Science, Nanjing Agricultural University, Weigang 1, Nanjing, 
      Jiangsu 210095, PR China. Electronic address: 2014816155@njau.edu.cn.
FAU - Chen, Ya T
AU  - Chen YT
AD  - College of Life Science, Nanjing Agricultural University, Weigang 1, Nanjing, 
      Jiangsu 210095, PR China. Electronic address: 2013816153@njau.edu.cn.
FAU - Zhang, Ke Y
AU  - Zhang KY
AD  - College of Life Science, Nanjing Agricultural University, Weigang 1, Nanjing, 
      Jiangsu 210095, PR China. Electronic address: keyunzhang@njau.edu.cn.
FAU - Zeng, Yan
AU  - Zeng Y
AD  - College of Life Science, Nanjing Agricultural University, Weigang 1, Nanjing, 
      Jiangsu 210095, PR China. Electronic address: zengyan@njau.edu.cn.
FAU - Yang, Qian
AU  - Yang Q
AD  - College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, 
      Nanjing, Jiangsu 210095, PR China. Electronic address: zxbyq@njau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170104
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (CD40 Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Interleukin-6)
RN  - 0 (MIRN29 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Oligoribonucleotides, Antisense)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Regulatory Factor X Transcription Factors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (interleukin-6, mouse)
RN  - 136628-24-5 (trans-activation responsive RNA-binding protein)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Bone Marrow Cells/immunology/virology
MH  - CD40 Antigens/genetics/immunology
MH  - Dendritic Cells/*immunology/virology
MH  - Gene Expression Regulation
MH  - Histocompatibility Antigens Class II/genetics/immunology
MH  - *Host-Pathogen Interactions
MH  - Influenza A Virus, H9N2 Subtype/*genetics/immunology/pathogenicity
MH  - Interleukin-6/genetics/*immunology
MH  - Lymphocyte Activation
MH  - Lymphocytes/immunology/virology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/agonists/antagonists & inhibitors/*genetics/immunology
MH  - Oligoribonucleotides, Antisense/genetics/metabolism
MH  - Primary Cell Culture
MH  - RNA-Binding Proteins/genetics/immunology
MH  - Regulatory Factor X Transcription Factors/genetics/immunology
MH  - Signal Transduction
MH  - Tumor Necrosis Factor-alpha/genetics/*immunology
MH  - Virulence
MH  - Virus Replication
OTO - NOTNLM
OT  - Dendritic cells
OT  - H9N2 AIV
OT  - Immune regulation
OT  - Target gene
OT  - miR29c
EDAT- 2017/01/09 06:00
MHDA- 2017/12/09 06:00
CRDT- 2017/01/09 06:00
PHST- 2016/09/18 00:00 [received]
PHST- 2016/12/19 00:00 [revised]
PHST- 2016/12/22 00:00 [accepted]
PHST- 2017/01/09 06:00 [pubmed]
PHST- 2017/12/09 06:00 [medline]
PHST- 2017/01/09 06:00 [entrez]
AID - S0264-410X(16)31287-7 [pii]
AID - 10.1016/j.vaccine.2016.12.054 [doi]
PST - ppublish
SO  - Vaccine. 2017 Feb 1;35(5):729-737. doi: 10.1016/j.vaccine.2016.12.054. Epub 2017 
      Jan 4.