PMID- 28066137
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1226-4512 (Print)
IS  - 2093-3827 (Electronic)
IS  - 1226-4512 (Linking)
VI  - 21
IP  - 1
DP  - 2017 Jan
TI  - Betulin suppressed interleukin-1beta-induced gene expression, secretion and 
      proteolytic activity of matrix metalloproteinase in cultured articular 
      chondrocytes and production of matrix metalloproteinase in the knee joint of rat.
PG  - 19-26
LID - 10.4196/kjpp.2017.21.1.19 [doi]
AB  - We investigated whether betulin affects the gene expression, secretion and 
      proteolytic activity of matrix metalloproteinase-3 (MMP-3) in primary cultured 
      rabbit articular chondrocytes, as well as in vivo production of MMP-3 in the rat 
      knee joint to evaluate the potential chondroprotective effect of betulin. Rabbit 
      articular chondrocytes were cultured and reverse transcription-polymerase chain 
      reaction (RT-PCR) was used to measure interleukin-1beta (IL-1beta)-induced gene 
      expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with 
      thrombospondin motifs-4 (ADAMTS-4), ADAMTS-5 and type II collagen. Effect of 
      betulin on IL-1beta-induced secretion and proteolytic activity of MMP-3 was 
      investigated using western blot analysis and casein zymography, respectively. 
      Effect of betulin on MMP-3 protein production was also examined in vivo. The 
      results were as follows: (1) betulin inhibited the gene expression of MMP-3, 
      MMP-1, MMP-13, ADAMTS-4, and ADAMTS-5, but increased the gene expression of type 
      II collagen; (2) betulin inhibited the secretion and proteolytic activity of 
      MMP-3; (3) betulin suppressed the production of MMP-3 protein in vivo. These 
      results suggest that betulin can regulate the gene expression, secretion, and 
      proteolytic activity of MMP-3, by directly acting on articular chondrocytes.
FAU - Ra, Ho Jong
AU  - Ra HJ
AD  - Department of Orthopedic Surgery, Gangneung Asan Hospital, College of Medicine, 
      University of Ulsan, Gangneung 25440, Korea.
FAU - Lee, Hyun Jae
AU  - Lee HJ
AD  - Department of Health Management, Sahmyook University, Seoul 01795, Korea.
FAU - Jo, Ho Seung
AU  - Jo HS
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea.
FAU - Nam, Dae Cheol
AU  - Nam DC
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea.
FAU - Lee, Young Bok
AU  - Lee YB
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea.
FAU - Kang, Byeong Hun
AU  - Kang BH
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea.
FAU - Moon, Dong Kyu
AU  - Moon DK
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea.
FAU - Kim, Dong Hee
AU  - Kim DH
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea.
FAU - Lee, Choong Jae
AU  - Lee CJ
AD  - Department of Pharmacology, School of Medicine, Chungnam National University, 
      Daejeon 35015, Korea.
FAU - Hwang, Sun-Chul
AU  - Hwang SC
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea.
LA  - eng
PT  - Journal Article
DEP - 20161221
PL  - Korea (South)
TA  - Korean J Physiol Pharmacol
JT  - The Korean journal of physiology & pharmacology : official journal of the Korean 
      Physiological Society and the Korean Society of Pharmacology
JID - 9709505
EIN - Korean J Physiol Pharmacol. 2017 Mar;21(2):275. PMID: 28280422
PMC - PMC5214907
OTO - NOTNLM
OT  - Betulin
OT  - Chondrocyte
OT  - Metalloproteinase
OT  - Osteoarthritis
COIS- The authors have no conflicts of interest to declare.
EDAT- 2017/01/10 06:00
MHDA- 2017/01/10 06:01
PMCR- 2017/01/01
CRDT- 2017/01/10 06:00
PHST- 2016/06/15 00:00 [received]
PHST- 2016/08/18 00:00 [revised]
PHST- 2016/08/19 00:00 [accepted]
PHST- 2017/01/10 06:00 [entrez]
PHST- 2017/01/10 06:00 [pubmed]
PHST- 2017/01/10 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 10.4196/kjpp.2017.21.1.19 [doi]
PST - ppublish
SO  - Korean J Physiol Pharmacol. 2017 Jan;21(1):19-26. doi: 10.4196/kjpp.2017.21.1.19. 
      Epub 2016 Dec 21.