PMID- 28073411
OWN - NLM
STAT- MEDLINE
DCOM- 20170928
LR  - 20181202
IS  - 1007-3418 (Print)
IS  - 1007-3418 (Linking)
VI  - 24
IP  - 12
DP  - 2016 Dec 20
TI  - [Effect of umbilical cord mesenchymal stem cell transplantation on immune 
      function and prognosis of patients with decompensated hepatitis B cirrhosis].
PG  - 907-910
LID - 10.3760/cma.j.issn.1007-3418.2016.12.006 [doi]
AB  - Objective: To investigate the effect of human umbilical cord mesenchymal stem 
      cells (hUCMSCs) on the immune function and prognosis of patients with 
      decompensated hepatitis B cirrhosis. Methods: A total of 65 patients with 
      decompensated hepatitis B cirrhosis were divided into observation group and 
      control group. The patients in the observation group were given intervention (via 
      the proper hepatic artery or the portal vein) and intravenous infusion of 4x10(8) 
      hUCMSCs in two doses, as well as the same basic treatment as in the control 
      group. The patients in the control group were given conventional medical 
      treatment. ELISA as used to measure the serum levels of interleukin-6 (IL-6), 
      tumor necrosis factor-alpha (TNFalpha), interleukin-10 (IL-10), and transforming growth 
      factor-beta (TGFbeta) in the observation group before surgery and at 1 week after 
      surgery, as well as the serum levels of IL-6, TNFalpha, IL-10, and TGFbeta in the 
      control group on admission and at 1 week after admission. Flow cytometry was used 
      to measure the percentage of lymphocyte subsets in the observation group before 
      surgery and at 1 week after surgery, as well as that in the control group on 
      admission and at 1 week after admission. In addition, the patients' prognosis and 
      major complications during hospitalization were observed in both groups, and the 
      patients were followed up for 24 weeks to record the number of deaths. The t-test 
      was used for comparison of continuous data, and the chi-square test was used for 
      comparison of categorical data which were expressed as percentages. Results: At 1 
      week after the transplantation of hUCMSCs, compared with the control group, the 
      observation group had significant reductions in the serum levels of IL-6 and TNFalpha 
      and significant increases in the serum levels of IL-10 and TGFbeta (all P < 0.001), 
      as well as significant increases in the percentages of T4 cells and Treg cells 
      and significant reductions in the percentages of T8 cells and B cells (all P < 
      0.05). There were no significant differences in the changes in T3 cells and 
      natural killer cells between the two groups (P > 0.05). Compared with the control 
      group, the observation group had a significantly lower probability of progression 
      to liver failure (6.45% vs 14.71%, P = 0.017). Conclusion: In the treatment of 
      patients with decompensated hepatitis B cirrhosis, transplantation of UCMSCs can 
      inhibit the proliferation of T cells and B cells and the differentiation of T8 
      cells, upregulate Treg cells, promote the secretion of immunosuppressive 
      cytokines, and reduce the production of inflammatory cytokines. Therefore, it can 
      alleviate liver inflammatory response and liver cell damage and reduce the 
      probability of hepatic failure.
FAU - Fang, X Q
AU  - Fang XQ
AD  - Clinical College of PLA of Anhui Medical University, Hefei 230001, China.
FAU - Zhang, J F
AU  - Zhang JF
AD  - The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
FAU - Song, H Y
AU  - Song HY
AD  - The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
FAU - Chen, Z L
AU  - Chen ZL
AD  - The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
FAU - Dong, J
AU  - Dong J
AD  - The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
FAU - Chen, X
AU  - Chen X
AD  - The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
FAU - Pan, J J
AU  - Pan JJ
AD  - The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
FAU - Liu, B
AU  - Liu B
AD  - The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
FAU - Chen, C X
AU  - Chen CX
AD  - The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
LA  - chi
PT  - Journal Article
PT  - Observational Study
PT  - Randomized Controlled Trial
PL  - China
TA  - Zhonghua Gan Zang Bing Za Zhi
JT  - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of 
      hepatology
JID - 9710009
RN  - 0 (Cytokines)
RN  - 0 (IL10 protein, human)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - B-Lymphocytes
MH  - Cell Differentiation
MH  - Cytokines/*blood
MH  - Flow Cytometry
MH  - Hepatitis B/blood/*surgery
MH  - Humans
MH  - Interleukin-10/blood
MH  - Interleukin-6/blood
MH  - Liver Cirrhosis/blood/*surgery
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells
MH  - Prognosis
MH  - T-Lymphocytes, Regulatory
MH  - Transforming Growth Factor beta/blood
MH  - Tumor Necrosis Factor-alpha/blood
MH  - *Umbilical Cord
EDAT- 2017/01/12 06:00
MHDA- 2017/09/29 06:00
CRDT- 2017/01/12 06:00
PHST- 2017/01/12 06:00 [entrez]
PHST- 2017/01/12 06:00 [pubmed]
PHST- 2017/09/29 06:00 [medline]
AID - 10.3760/cma.j.issn.1007-3418.2016.12.006 [doi]
PST - ppublish
SO  - Zhonghua Gan Zang Bing Za Zhi. 2016 Dec 20;24(12):907-910. doi: 
      10.3760/cma.j.issn.1007-3418.2016.12.006.