PMID- 28074628
OWN - NLM
STAT- MEDLINE
DCOM- 20180723
LR  - 20180723
IS  - 1476-5829 (Electronic)
IS  - 1476-5810 (Linking)
VI  - 15
IP  - 4
DP  - 2017 Dec
TI  - S100A4 (metastasin) positive mesenchymal canine mammary tumour spheroids reduce 
      Tenascin C synthesis under DMSO exposure in vitro.
PG  - 1428-1444
LID - 10.1111/vco.12287 [doi]
AB  - In breast cancer research S100A4-positive tumour-associated stromal cells are 
      assumed as primary source of Tenascin C (TNC) in the metastatic environment. Aim 
      of the present study was to isolate and characterize S100A4/TNC positive stromal 
      canine mammary tumour (CMT) cells. Cells grown as scaffold-free spheroids were 
      investigated for S100A4, TNC, and proliferative activity under 1.8% DMSO 
      stimulation by means of Western blot and immunohistochemistry. DMSO is a commonly 
      used drug solvent despite well-known side effects on cells including TNC 
      expression. DMSO did not affect proliferation, but TNC was significantly reduced 
      under DMSO exposure for 7 and 14 days, whereby for S100A4 a reducing effect was 
      only observed after 14 days. Without DMSO, cells stably expressed TNC and S100A4 
      which makes them suitable to be used in experimental approaches requiring 
      S100A4/TNC expressing CMT stromal cells. Results show that 1.8% DMSO should not 
      be used as solvent for experiments concerning TNC/S100A4 expression.
CI  - (c) 2017 John Wiley & Sons Ltd.
FAU - Kau, S
AU  - Kau S
AD  - Institute of Anatomy, Histology and Embryology, Department of Pathobiology, 
      University of Veterinary Medicine, Vienna, Austria.
FAU - Miller, I
AU  - Miller I
AD  - Institute for Medical Biochemistry, Department for Biomedical Sciences, 
      University of Veterinary Medicine, Vienna, Austria.
FAU - Tichy, A
AU  - Tichy A
AD  - Platform Biostatistics, Department of Biomedical Science, Institute of Population 
      Genetics, University of Veterinary Medicine, Vienna, Austria.
FAU - Gabriel, C
AU  - Gabriel C
AD  - Institute of Anatomy, Histology and Embryology, Department of Pathobiology, 
      University of Veterinary Medicine, Vienna, Austria.
LA  - eng
PT  - Journal Article
DEP - 20170111
PL  - England
TA  - Vet Comp Oncol
JT  - Veterinary and comparative oncology
JID - 101185242
RN  - 0 (S100 Calcium-Binding Protein A4)
RN  - 0 (Tenascin)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Animals
MH  - Blotting, Western/veterinary
MH  - Dimethyl Sulfoxide/*pharmacology
MH  - Dog Diseases/*metabolism
MH  - Dogs
MH  - Female
MH  - Mammary Neoplasms, Animal/*metabolism
MH  - S100 Calcium-Binding Protein A4/*metabolism
MH  - Spheroids, Cellular/drug effects/metabolism
MH  - Tenascin/*metabolism
MH  - Tumor Cells, Cultured/drug effects/metabolism
OTO - NOTNLM
OT  - DMSO
OT  - S100A4
OT  - TNC
OT  - canine mammary tumour
OT  - tumour stroma
EDAT- 2017/01/12 06:00
MHDA- 2018/07/24 06:00
CRDT- 2017/01/12 06:00
PHST- 2016/05/17 00:00 [received]
PHST- 2016/09/21 00:00 [revised]
PHST- 2016/10/16 00:00 [accepted]
PHST- 2017/01/12 06:00 [pubmed]
PHST- 2018/07/24 06:00 [medline]
PHST- 2017/01/12 06:00 [entrez]
AID - 10.1111/vco.12287 [doi]
PST - ppublish
SO  - Vet Comp Oncol. 2017 Dec;15(4):1428-1444. doi: 10.1111/vco.12287. Epub 2017 Jan 
      11.