PMID- 28075448
OWN - NLM
STAT- MEDLINE
DCOM- 20170316
LR  - 20191210
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 39
IP  - 2
DP  - 2017 Feb
TI  - Microarray analysis of circular RNA expression patterns in polarized macrophages.
PG  - 373-379
LID - 10.3892/ijmm.2017.2852 [doi]
AB  - Circular RNAs (circRNAs) are generated from diverse genomic locations and are a 
      new player in the regulation of post-transcriptional gene expression. Recent 
      studies have revealed that circRNAs play a crucial role in fine-tuning the level 
      of microRNA (miRNA)-mediated regulation of gene expression by sequestering 
      miRNAs. The interaction of circRNAs with disease-associated miRNAs suggests that 
      circRNAs are important in the pathology of disease. However, the effects and 
      roles of circRNAs in macrophage polarization have yet to be explored. In the 
      present study, we performed a circRNA microarray to compare the circRNA 
      expression profiles of bone marrow-derived macrophages (BMDMs) under two distinct 
      polarizing conditions (M1 macrophages induced by interferon-gamma and LPS 
      stimulation, and M2 macrophages induced by interleukin-4 stimulation). Our 
      results showed that a total of 189 circRNAs were differentially expressed between 
      M1 and M2 macrophages. Differentially expressed circRNAs with a high fold-change 
      were selected for validation by RT-qPCR: circRNA-003780, circRNA-010056, and 
      circRNA-010231 were upregulated and circRNA-003424, circRNA-013630, 
      circRNA-001489 and circRNA-018127 were downregulated (fold-change >4, P<0.05) in 
      M1 compared to M2, which was found to correlate with the microarray data. 
      Furthermore, the most differentially expressed circRNAs within all the 
      comparisons were annotated in detail with circRNA/miRNA interaction information 
      using miRNA target prediction software. In conclusion, the present study provides 
      novel insight into the role of circRNAs in macrophage differentiation and 
      polarization.
FAU - Zhang, Yingying
AU  - Zhang Y
AD  - Laboratory Medicine, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui 
      241001, P.R. China.
FAU - Zhang, Yao
AU  - Zhang Y
AD  - Department of Biochemistry, Wannan Medical College, Wuhu, Anhui 241001, P.R. 
      China.
FAU - Li, Xueqin
AU  - Li X
AD  - Central Laboratory, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui 
      241001, P.R. China.
FAU - Zhang, Mengying
AU  - Zhang M
AD  - Central Laboratory, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui 
      241001, P.R. China.
FAU - Lv, Kun
AU  - Lv K
AD  - Central Laboratory, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui 
      241001, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170111
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Cluster Analysis
MH  - Computational Biology/methods
MH  - Epistasis, Genetic
MH  - *Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Macrophages/metabolism
MH  - Male
MH  - Mice
MH  - MicroRNAs/genetics
MH  - Molecular Sequence Annotation
MH  - RNA/*genetics
MH  - RNA Interference
MH  - RNA, Circular
MH  - *Transcriptome
PMC - PMC5358696
EDAT- 2017/01/12 06:00
MHDA- 2017/03/17 06:00
PMCR- 2017/01/11
CRDT- 2017/01/12 06:00
PHST- 2015/11/30 00:00 [received]
PHST- 2017/01/05 00:00 [accepted]
PHST- 2017/01/12 06:00 [pubmed]
PHST- 2017/03/17 06:00 [medline]
PHST- 2017/01/12 06:00 [entrez]
PHST- 2017/01/11 00:00 [pmc-release]
AID - ijmm-39-02-0373 [pii]
AID - 10.3892/ijmm.2017.2852 [doi]
PST - ppublish
SO  - Int J Mol Med. 2017 Feb;39(2):373-379. doi: 10.3892/ijmm.2017.2852. Epub 2017 Jan 
      11.