PMID- 28077616
OWN - NLM
STAT- MEDLINE
DCOM- 20170919
LR  - 20181113
IS  - 1939-4586 (Electronic)
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 28
IP  - 5
DP  - 2017 Mar 1
TI  - Different modes of enhancer-specific regulation by Runt and Even-skipped during 
      Drosophila segmentation.
PG  - 681-691
LID - 10.1091/mbc.E16-09-0630 [doi]
AB  - The initial metameric expression of the Drosophila sloppy paired 1 (slp1) gene is 
      controlled by two distinct cis-regulatory DNA elements that interact in a 
      nonadditive manner to integrate inputs from transcription factors encoded by the 
      pair-rule segmentation genes. We performed chromatin immunoprecipitation on 
      reporter genes containing these elements in different embryonic genotypes to 
      investigate the mechanism of their regulation. The distal early stripe element 
      (DESE) mediates both activation and repression by Runt. We find that the 
      differential response of DESE to Runt is due to an inhibitory effect of Fushi 
      tarazu (Ftz) on P-TEFb recruitment and the regulation of RNA polymerase II (Pol 
      II) pausing. The proximal early stripe element (PESE) is also repressed by Runt, 
      but in this case, Runt prevents PESE-dependent Pol II recruitment and 
      preinitiation complex (PIC) assembly. PESE is also repressed by Even-skipped 
      (Eve), but, of interest, this repression involves regulation of P-TEFb 
      recruitment and promoter-proximal Pol II pausing. These results demonstrate that 
      the mode of slp1 repression by Runt is enhancer specific, whereas the mode of 
      repression of the slp1 PESE enhancer is transcription factor specific. We propose 
      a model based on these differential regulatory interactions that accounts for the 
      nonadditive interactions between the PESE and DESE enhancers during Drosophila 
      segmentation.
CI  - (c) 2017 Hang and Gergen. This article is distributed by The American Society for 
      Cell Biology under license from the author(s). Two months after publication it is 
      available to the public under an Attribution-Noncommercial-Share Alike 3.0 
      Unported Creative Commons License 
      (http://creativecommons.org/licenses/by-nc-sa/3.0).
FAU - Hang, Saiyu
AU  - Hang S
AD  - Department of Biochemistry and Cell Biology and Center for Developmental Genetics 
      and.
AD  - Graduate Program in Biochemistry and Structural Biology, Stony Brook University, 
      Stony Brook, NY 11794.
FAU - Gergen, J Peter
AU  - Gergen JP
AD  - Department of Biochemistry and Cell Biology and Center for Developmental Genetics 
      and john.peter.gergen@stonybrook.edu.
LA  - eng
GR  - R01 GM094401/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20170111
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Fushi Tarazu Transcription Factors)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (eve protein, Drosophila)
RN  - 0 (ftz protein, Drosophila)
RN  - 0 (run protein, Drosophila)
RN  - 0 (slp1 protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Body Patterning/genetics
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Drosophila/embryology/genetics/metabolism
MH  - Drosophila Proteins/genetics/*metabolism
MH  - Embryo, Nonmammalian/metabolism
MH  - Female
MH  - Fushi Tarazu Transcription Factors/genetics/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Genes, Insect
MH  - Homeodomain Proteins/genetics/*metabolism
MH  - Male
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Promoter Regions, Genetic
MH  - Regulatory Sequences, Nucleic Acid
MH  - Transcription Factors/genetics/*metabolism
PMC - PMC5328626
EDAT- 2017/01/13 06:00
MHDA- 2017/09/20 06:00
PMCR- 2017/05/16
CRDT- 2017/01/13 06:00
PHST- 2016/09/02 00:00 [received]
PHST- 2016/12/13 00:00 [revised]
PHST- 2017/01/04 00:00 [accepted]
PHST- 2017/01/13 06:00 [pubmed]
PHST- 2017/09/20 06:00 [medline]
PHST- 2017/01/13 06:00 [entrez]
PHST- 2017/05/16 00:00 [pmc-release]
AID - mbc.E16-09-0630 [pii]
AID - E16-09-0630 [pii]
AID - 10.1091/mbc.E16-09-0630 [doi]
PST - ppublish
SO  - Mol Biol Cell. 2017 Mar 1;28(5):681-691. doi: 10.1091/mbc.E16-09-0630. Epub 2017 
      Jan 11.