PMID- 28078845
OWN - NLM
STAT- MEDLINE
DCOM- 20170316
LR  - 20170316
IS  - 0393-974X (Print)
IS  - 0393-974X (Linking)
VI  - 30
IP  - 4
DP  - 2016 Oct-Dec
TI  - Effect of low energy light irradiation by light emitting diode on U937 cells.
PG  - 997-1007
AB  - Photobiomodulation (PBM) can induce a set of different biological modulators 
      either in vitro or in vivo. Experimental evidence has highlighted the role of 
      light effects on the mechanisms related to inflammation, apoptosis and autophagy. 
      The goal of this project was the evaluation of PBM on U937, an established cell 
      line of histiocytic lymphoma origin. Several aspects of modulation of 
      proinflammatory pathways were analyzed and autophagic and proapoptotic mechanisms 
      related to low laser light exposure of cells were studied. As a source of low 
      energy light emission, we used an NIR-LED device, characterized by an 880 
      nm-wavelength as light source. Flow cytometry analysis was performed on 
      supernatants of controls and treated U937 cells to detect inflammatory cytokine 
      levels. In order to evaluate NF-kB and caspase3 expressions, Western blot 
      analysis was performed according to standard procedures. In this report, we show 
      the effect of PBM on a monocyte/macrophage established tumor cell line (U-937). 
      We demonstrate that LED exposure, in the presence or absence of 
      lipopolysaccharide (LPS), activates cell degranulation, increased expression of 
      Interleukin-8 (IL-8) and modulation of beta galactosidase activity. Evidence 
      shows that the well-known pro-inflammatory nuclear factor 
      kappa-light-chain-enhancer of activated B cells (NF-kB) and the apoptotic marker 
      (caspase3/cleaved-caspase3 ratio) are up-regulated in response to a 
      proinflammatory biochemical pathway.
FAU - Spoto, G
AU  - Spoto G
AD  - Department of Medical, Oral and Biotechnological Sciences, University G. 
      d’Annunzio of Chieti, Italy.
FAU - De Iuliis, V
AU  - De Iuliis V
AD  - Unit of Predictive Medicine and Clinical Molecular biology, SS Annunziata 
      University Hospital, ASL Lanciano-Vasto-Chieti, University G d’Annunzio, Chieti, 
      Italy.
FAU - Petrini, M
AU  - Petrini M
AD  - Department of Medical, Oral and Biotechnological Sciences, University G. 
      d’Annunzio of Chieti, Italy.
FAU - Flati, V
AU  - Flati V
AD  - Department of Biotechnological and Applied Clinical Sciences, University of 
      L’Aquila, L’Aquila, Italy.
FAU - Di Gregorio, J
AU  - Di Gregorio J
AD  - Department of Biotechnological and Applied Clinical Sciences, University of 
      L’Aquila, L’Aquila, Italy.
FAU - Vitale, D
AU  - Vitale D
AD  - Department of Medical, Oral and Biotechnological Sciences, University G. 
      d’Annunzio of Chieti, Italy.
FAU - Caruso, M
AU  - Caruso M
AD  - Department of Medical, Oral and Biotechnological Sciences, University G. 
      d’Annunzio of Chieti, Italy.
FAU - Dadorante, V
AU  - Dadorante V
AD  - Unit of Predictive Medicine and Clinical Molecular biology, SS Annunziata 
      University Hospital, ASL Lanciano-Vasto-Chieti, University G d’Annunzio, Chieti, 
      Italy.
FAU - Ciarmoli, M
AU  - Ciarmoli M
AD  - CNR, Institute of Molecular Genetics, Section of Chieti, Chieti, Italy.
FAU - Robuffo, I
AU  - Robuffo I
AD  - CNR, Institute of Molecular Genetics, Section of Chieti, Chieti, Italy.
FAU - Martinotti, S
AU  - Martinotti S
AD  - Unit of Predictive Medicine and Clinical Molecular biology, SS Annunziata 
      University Hospital, ASL Lanciano-Vasto-Chieti, University G d’Annunzio, Chieti, 
      Italy.
FAU - Toniato, E
AU  - Toniato E
AD  - Department of Medical, Oral and Biotechnological Sciences, University G. 
      d’Annunzio of Chieti, Italy.
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - J Biol Regul Homeost Agents
JT  - Journal of biological regulators and homeostatic agents
JID - 8809253
RN  - 0 (Interleukin-8)
RN  - 0 (NF-kappa B)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Apoptosis/*radiation effects
MH  - Blotting, Western
MH  - Caspase 3/metabolism
MH  - Cell Degranulation/*radiation effects
MH  - Flow Cytometry
MH  - Humans
MH  - Inflammation/metabolism
MH  - Interleukin-8/metabolism
MH  - *Low-Level Light Therapy
MH  - Macrophages/metabolism/*radiation effects
MH  - Monocytes/metabolism/*radiation effects
MH  - NF-kappa B/metabolism
MH  - U937 Cells
EDAT- 2017/01/13 06:00
MHDA- 2017/03/17 06:00
CRDT- 2017/01/13 06:00
PHST- 2017/01/13 06:00 [entrez]
PHST- 2017/01/13 06:00 [pubmed]
PHST- 2017/03/17 06:00 [medline]
AID - 7 [pii]
PST - ppublish
SO  - J Biol Regul Homeost Agents. 2016 Oct-Dec;30(4):997-1007.