PMID- 28081279
OWN - NLM
STAT- MEDLINE
DCOM- 20170316
LR  - 20170316
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 15
IP  - 4
DP  - 2016 Dec 23
TI  - Karyotype analysis and ribosomal gene localization of spotted knifejaw 
      Oplegnathus punctatus.
LID - 10.4238/gmr15049159 [doi]
AB  - The spotted knifejaw, Oplegnathus punctatus, is an important aquaculture fish 
      species in China. To better understand the chromosomal microstructure and the 
      karyotypic origin of this species, cytogenetic analysis was performed using 
      Giemsa staining to identify metaphase chromosomes, C-banding to detect C-positive 
      heterochromatin, silver staining to identify the nucleolus organizer regions 
      (Ag-NORs), and fluorescence in situ hybridization (FISH) for physical mapping of 
      the major (18S rDNA) and minor (5S rDNA) ribosomal genes. The species showed a 
      karyotype of 2n = 48 for females, composed of 2 submetacentric and 46 telocentric 
      chromosomes, with a fundamental number (FN) = 50, while the karyotype of males 
      was 2n = 47, composed of 1 exclusive large metacentric, 2 submetacentric, and 44 
      telocentric chromosomes, with FN = 50. These karyotype results suggest that O. 
      punctatus might have an X(1)X(1)X(2)X(2)/X(1)X(2)Y multiple sex chromosome 
      system. C-positive heterochromatin was distributed in the centromeres of all 
      chromosomal pairs and in the terminal portions of some chromosomes. A single pair 
      of Ag-positive NORs was found to be localized at the terminal regions of the 
      short arms of the subtelocentric chromosome pair, which was supported by FISH of 
      18S rDNA. After FISH, 5S rDNA were located on the interstitial regions of the 
      smallest telocentric chromosome pair. This study was the first to identify the 
      karyotype of this species and will facilitate further research on karyotype 
      evolution in the order Perciformes.
FAU - Li, P Z
AU  - Li PZ
AD  - Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, 
      Ocean University of China, Ministry of Education, Qingdao, China.
FAU - Cao, D D
AU  - Cao DD
AD  - Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, 
      Ocean University of China, Ministry of Education, Qingdao, China.
FAU - Liu, X B
AU  - Liu XB
AD  - Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, 
      Ocean University of China, Ministry of Education, Qingdao, China.
FAU - Wang, Y J
AU  - Wang YJ
AD  - Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, 
      Ocean University of China, Ministry of Education, Qingdao, China.
FAU - Yu, H Y
AU  - Yu HY
AD  - Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, 
      Ocean University of China, Ministry of Education, Qingdao, China.
FAU - Li, X J
AU  - Li XJ
AD  - Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, 
      Ocean University of China, Ministry of Education, Qingdao, China.
FAU - Zhang, Q Q
AU  - Zhang QQ
AD  - Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, 
      Ocean University of China, Ministry of Education, Qingdao, China.
FAU - Wang, X B
AU  - Wang XB
AD  - Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, 
      Ocean University of China, Ministry of Education, Qingdao, China 
      wangxubo@ouc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20161223
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - DNA, Ribosomal/*genetics
MH  - Fishes/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *Karyotype
MH  - Karyotyping
MH  - Nucleolus Organizer Region
EDAT- 2017/01/13 06:00
MHDA- 2017/03/17 06:00
CRDT- 2017/01/13 06:00
PHST- 2017/01/13 06:00 [entrez]
PHST- 2017/01/13 06:00 [pubmed]
PHST- 2017/03/17 06:00 [medline]
AID - gmr-15-04-gmr.15049159 [pii]
AID - 10.4238/gmr15049159 [doi]
PST - epublish
SO  - Genet Mol Res. 2016 Dec 23;15(4). doi: 10.4238/gmr15049159.