PMID- 28081700
OWN - NLM
STAT- MEDLINE
DCOM- 20170823
LR  - 20181113
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 14
IP  - 1
DP  - 2017 Jan 11
TI  - HHV-6A in vitro infection of thyrocytes and T cells alters the expression of 
      miRNA associated to autoimmune thyroiditis.
PG  - 3
LID - 10.1186/s12985-016-0672-6 [doi]
LID - 3
AB  - BACKGROUND: Human herpesviruses have been hypothesized as environmental triggers 
      in the development of autoimmune thyroid diseases (AITD), and in particular 
      active human herpesvirus 6A (HHV-6A) infection was detected in thyrocytes of 
      Hashimoto's thyroiditis (HT) patients, who also show specific anti-viral immune 
      responses. On the other hand, AITD patients display modulation of specific miRNAs 
      in thyroid tissue and blood. We wanted to ascertain whether HHV-6A infection 
      might be correlated to the miRNA dysregulation observed in AITD. METHODS: Human 
      thyroid and T-cell lines were infected in vitro with HHV-6A,-6B or -7, and 
      analysed for miRNAs expression, either by microarray or by specific RT-PCR assays 
      detecting miRNAs associated with AITD in vivo. RESULTS: HHV-6A infection, but not 
      -6B or -7 infections, induced a decrease in miR-155_2 expression and an increase 
      in miR-1238 expression in thyrocytes, as well as an increase in the expression 
      levels of several autoimmunity-associated miRNAs in T lymphocytes, including 
      miR-16_1, miR34a, miR-130a, miR-143_1, miR-202, miR-301b, miR-302c, miR-449b, 
      miR-451_1, and miR-1238_2. CONCLUSIONS: HHV-6A infection modulates miRNAs 
      expression in the cell types involved in the development of AITD. Notably, our in 
      vitro findings correlate with what observed in AITD patients, further supporting 
      the association between HHV-6A infection and AITD development. Moreover, these 
      effects are 6A-specific, emphasizing the differences between the two HHV-6 virus 
      species, and suggesting diverse virus mechanisms of action and therapeutic 
      approaches.
FAU - Caselli, Elisabetta
AU  - Caselli E
AUID- ORCID: 0000-0001-6048-9141
AD  - Department of Medical Sciences, Section of Microbiology and Medical Genetics, 
      University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy. 
      elisabetta.caselli@unife.it.
FAU - D'Accolti, Maria
AU  - D'Accolti M
AD  - Department of Medical Sciences, Section of Microbiology and Medical Genetics, 
      University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy.
FAU - Soffritti, Irene
AU  - Soffritti I
AD  - Department of Medical Sciences, Section of Microbiology and Medical Genetics, 
      University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy.
FAU - Zatelli, Maria Chiara
AU  - Zatelli MC
AD  - Department of Medical Sciences, Section of Endocrinology and Internal Medicine, 
      University of Ferrara, via A. Moro 8, 44124, Ferrara, Italy.
FAU - Rossi, Roberta
AU  - Rossi R
AD  - Endocrinology Unit, Azienda Ospedaliero-Universitaria di Ferrara, via A. Moro 8, 
      44124, Ferrara, Italy.
FAU - Degli Uberti, Ettore
AU  - Degli Uberti E
AD  - Department of Medical Sciences, Section of Endocrinology and Internal Medicine, 
      University of Ferrara, via A. Moro 8, 44124, Ferrara, Italy.
FAU - Di Luca, Dario
AU  - Di Luca D
AD  - Department of Medical Sciences, Section of Microbiology and Medical Genetics, 
      University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170111
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Gene Expression Profiling
MH  - Herpesvirus 6, Human/*growth & development
MH  - Herpesvirus 7, Human/growth & development
MH  - Humans
MH  - MicroRNAs/*analysis
MH  - Microarray Analysis
MH  - Real-Time Polymerase Chain Reaction
MH  - T-Lymphocytes/*virology
MH  - Thyroid Epithelial Cells/*virology
MH  - Thyroiditis, Autoimmune/*pathology
PMC - PMC5234148
OTO - NOTNLM
OT  - Autoimmune thyroiditis
OT  - HHV-6
OT  - miRNA
EDAT- 2017/01/14 06:00
MHDA- 2017/08/24 06:00
PMCR- 2017/01/11
CRDT- 2017/01/14 06:00
PHST- 2016/10/13 00:00 [received]
PHST- 2016/12/15 00:00 [accepted]
PHST- 2017/01/14 06:00 [entrez]
PHST- 2017/01/14 06:00 [pubmed]
PHST- 2017/08/24 06:00 [medline]
PHST- 2017/01/11 00:00 [pmc-release]
AID - 10.1186/s12985-016-0672-6 [pii]
AID - 672 [pii]
AID - 10.1186/s12985-016-0672-6 [doi]
PST - epublish
SO  - Virol J. 2017 Jan 11;14(1):3. doi: 10.1186/s12985-016-0672-6.