PMID- 28085534
OWN - NLM
STAT- MEDLINE
DCOM- 20171110
LR  - 20220409
IS  - 1557-7732 (Electronic)
IS  - 1080-7683 (Print)
IS  - 1080-7683 (Linking)
VI  - 33
IP  - 3
DP  - 2017 Apr
TI  - Photoelectric Dye Used for Okayama University-Type Retinal Prosthesis Reduces the 
      Apoptosis of Photoreceptor Cells.
PG  - 149-160
LID - 10.1089/jop.2016.0093 [doi]
AB  - PURPOSE: Our previous study demonstrated that photoelectric dye-coupled 
      polyethylene film (Okayama University-type retinal prosthesis), which was 
      implanted in subretinal space of the eyes of Royal College of Surgeons (RCS) 
      rats, prevented retinal neurons from apoptotic death. In this study, we aimed to 
      examine whether photoelectric dye itself would protect retinal neurons from 
      apoptosis in RCS rats. METHODS: RCS rats received intravitreous injection of 
      different concentrations of the dye in the left eye and housed under a 12-h 
      light-dark cycle. Saline injection in the right eye served as control. In 
      addition, RCS rats with dye injection were kept in 24-h daily dark condition. 
      Sections were processed for terminal deoxynucleotidyl transferase-mediated 
      fluorescein-conjugated-dUTP nick-end-labeling (TUNEL) assay and 
      immunohistochemical staining of glial fibrillary acidic protein (GFAP) and 
      protein kinase Calpha (PKCalpha). RESULTS: The number of TUNEL-positive cells 
      significantly decreased in the retina of dye-injected eyes compared with those in 
      saline-injected eyes (P = 0.0001, 2-factor analysis of variance [ANOVA]), under 
      12-h light-dark cycle. Significant decrease of TUNEL-positive cells was noted in 
      the retina of rats with dye injection compared with those with saline injection, 
      kept under 24-h dark condition (P = 0.0001, 2-factor ANOVA). Immunoreactive area 
      for GFAP decreased significantly in the retina of dye-injected eyes compared with 
      that in controls (P = 0.0001, 2-factor ANOVA), whereas immunoreactive area for 
      PKCalpha increased significantly in the retina of dye-injected eyes compared with 
      that in controls (P = 0.01, 2-factor ANOVA). CONCLUSIONS: Photoelectric dye 
      inhibits apoptotic death of photoreceptor cells in RCS rats and downregulates 
      GFAP expression in retinal Muller cells. Photoelectric dye may be a candidate 
      agent for neuroprotection in retinitis pigmentosa and other retinal diseases.
FAU - Liu, Shihui
AU  - Liu S
AD  - 1 Department of Ophthalmology, Okayama University Medical School and Graduate 
      School of Medicine , Dentistry, and Pharmaceutical Sciences, Okayama City, Japan 
      .
FAU - Matsuo, Toshihiko
AU  - Matsuo T
AD  - 1 Department of Ophthalmology, Okayama University Medical School and Graduate 
      School of Medicine , Dentistry, and Pharmaceutical Sciences, Okayama City, Japan 
      .
FAU - Hosoya, Osamu
AU  - Hosoya O
AD  - 2 Department of Medical Neurobiology, Okayama University Medical School and 
      Graduate School of Medicine , Dentistry, and Pharmaceutical Sciences, Okayama 
      City, Japan .
FAU - Uchida, Tetsuya
AU  - Uchida T
AD  - 3 Polymer Materials Science, Okayama University Faculty of Engineering and 
      Graduate School of Natural Science and Technology , Okayama City, Japan .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170113
PL  - United States
TA  - J Ocul Pharmacol Ther
JT  - Journal of ocular pharmacology and therapeutics : the official journal of the 
      Association for Ocular Pharmacology and Therapeutics
JID - 9511091
RN  - 0 (Coloring Agents)
RN  - 9002-88-4 (Polyethylene)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Coloring Agents/chemistry/*pharmacology
MH  - Photoreceptor Cells/*drug effects
MH  - Polyethylene/chemistry/*pharmacology
MH  - Rats
MH  - Rats, Mutant Strains
MH  - Retina/*drug effects/physiopathology
MH  - *Visual Prosthesis
PMC - PMC5385417
OTO - NOTNLM
OT  - GFAP
OT  - PKCalpha
OT  - apoptosis
OT  - drug
OT  - photoreceptors
OT  - retina
COIS- No competing financial interests exist.
EDAT- 2017/01/14 06:00
MHDA- 2017/11/11 06:00
PMCR- 2017/04/01
CRDT- 2017/01/14 06:00
PHST- 2017/01/14 06:00 [pubmed]
PHST- 2017/11/11 06:00 [medline]
PHST- 2017/01/14 06:00 [entrez]
PHST- 2017/04/01 00:00 [pmc-release]
AID - 10.1089/jop.2016.0093 [pii]
AID - 10.1089/jop.2016.0093 [doi]
PST - ppublish
SO  - J Ocul Pharmacol Ther. 2017 Apr;33(3):149-160. doi: 10.1089/jop.2016.0093. Epub 
      2017 Jan 13.