PMID- 28087534
OWN - NLM
STAT- MEDLINE
DCOM- 20171109
LR  - 20181113
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 83
IP  - 7
DP  - 2017 Apr 1
TI  - Mechanisms of Horizontal Cell-to-Cell Transfer of Wolbachia spp. in Drosophila 
      melanogaster.
LID - 10.1128/AEM.03425-16 [doi]
LID - e03425-16
AB  - Wolbachia is an intracellular endosymbiont present in most arthropod and filarial 
      nematode species. Transmission between hosts is primarily vertical, taking place 
      exclusively through the female germ line, although horizontal transmission has 
      also been documented. The results of several studies indicate that Wolbachia spp. 
      can undergo transfer between somatic and germ line cells during nematode 
      development and in adult flies. However, the mechanisms underlying horizontal 
      cell-to-cell transfer remain largely unexplored. Here, we establish a tractable 
      system for probing horizontal transfer of Wolbachia cells between Drosophila 
      melanogaster cells in culture using fluorescence in situ hybridization (FISH). 
      First, we show that horizontal transfer is independent of cell-to-cell contact 
      and can efficiently take place through the culture medium within hours. Further, 
      we demonstrate that efficient transfer utilizes host cell phagocytic and 
      clathrin/dynamin-dependent endocytic machinery. Lastly, we provide evidence that 
      this process is conserved between species, showing that horizontal transfer from 
      mosquito to Drosophila cells takes place in a similar fashion. Altogether, our 
      results indicate that Wolbachia utilizes host internalization machinery during 
      infection, and this mechanism is conserved across insect species.IMPORTANCE Our 
      work has broad implications for the control and treatment of tropical diseases. 
      Wolbachia can confer resistance against a variety of human pathogens in mosquito 
      vectors. Elucidating the mechanisms of horizontal transfer will be useful for 
      efforts to more efficiently infect nonnatural insect hosts with Wolbachia as a 
      biological control agent. Further, as Wolbachia is essential for the survival of 
      filarial nematodes, understanding horizontal transfer might provide new 
      approaches to treating human infections by targeting Wolbachia Finally, this work 
      provides a key first step toward the genetic manipulation of Wolbachia.
CI  - Copyright (c) 2017 American Society for Microbiology.
FAU - White, Pamela M
AU  - White PM
AD  - Department of Molecular, Cell & Developmental Biology, University of California, 
      Santa Cruz, California, USA.
FAU - Pietri, Jose E
AU  - Pietri JE
AD  - Department of Molecular, Cell & Developmental Biology, University of California, 
      Santa Cruz, California, USA jose.e.pietri@gmail.com.
FAU - Debec, Alain
AU  - Debec A
AD  - Jacques Monod Institute, CNRS, Paris Diderot University, Paris, France.
FAU - Russell, Shelbi
AU  - Russell S
AD  - Department of Molecular, Cell & Developmental Biology, University of California, 
      Santa Cruz, California, USA.
FAU - Patel, Bhavin
AU  - Patel B
AD  - Department of Molecular, Cell & Developmental Biology, University of California, 
      Santa Cruz, California, USA.
FAU - Sullivan, William
AU  - Sullivan W
AD  - Department of Molecular, Cell & Developmental Biology, University of California, 
      Santa Cruz, California, USA.
LA  - eng
GR  - R01 GM104486/GM/NIGMS NIH HHS/United States
GR  - R25 GM056765/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170317
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Clathrin)
RN  - EC 3.6.5.5 (Dynamins)
SB  - IM
MH  - Animals
MH  - Clathrin/metabolism
MH  - Drosophila melanogaster/*cytology/*microbiology/physiology
MH  - Dynamins/metabolism
MH  - Germ Cells/microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Wolbachia/cytology/*physiology
PMC - PMC5359480
OTO - NOTNLM
OT  - Drosophila
OT  - Wolbachia
OT  - endocytosis
OT  - entry
OT  - horizontal
OT  - invasion
OT  - phagocytosis
OT  - transfer
OT  - transmission
EDAT- 2017/01/15 06:00
MHDA- 2017/11/10 06:00
PMCR- 2017/09/17
CRDT- 2017/01/15 06:00
PHST- 2016/12/22 00:00 [received]
PHST- 2017/01/10 00:00 [accepted]
PHST- 2017/01/15 06:00 [pubmed]
PHST- 2017/11/10 06:00 [medline]
PHST- 2017/01/15 06:00 [entrez]
PHST- 2017/09/17 00:00 [pmc-release]
AID - AEM.03425-16 [pii]
AID - 03425-16 [pii]
AID - 10.1128/AEM.03425-16 [doi]
PST - epublish
SO  - Appl Environ Microbiol. 2017 Mar 17;83(7):e03425-16. doi: 10.1128/AEM.03425-16. 
      Print 2017 Apr 1.