PMID- 28087905
OWN - NLM
STAT- MEDLINE
DCOM- 20170926
LR  - 20230805
IS  - 1008-9292 (Print)
IS  - 1008-9292 (Linking)
VI  - 45
IP  - 5
DP  - 2016 May 25
TI  - [Inflammatory cytokines and oxidative stress markers in the inhibition of 
      osteoarthritis by curcumin].
PG  - 461-468
AB  - Objective: To observe the influence of matrix metalloproteinases-2 (MMP-2), 
      monocyte chemoattractant protein-1 (MCP-1), CD47, L-selectin and advanced 
      oxidation proteinproducts (AOPP) in osteoarthritis and the intervention of 
      curcumin. Methods: A total of 20 male C57BL/6 mice (10.05-15.00 g) were randomly 
      divided into control group, OA group, Cur25 group and Cur50 group 
      (intraperitoneal injected 25 mumol/L or 50 mumol/L of curcumin everyday after 
      modeling). After 4 weeks treatment, we observed the morphological changes of the 
      gross specimen by immunohistochemical method, and observed the ultrastructure of 
      cartilage tissue under electron microscope. The expression of MMP-2, MCP-1 and 
      CD47 were detected by western blotting, and L-selectin and AOPP were detected by 
      ELISA and spectrophotometer, respectively. Results: In the cartilage tissue 
      morphology, the chondrocytes of OA group showed obvious change, while Cur25 and 
      Cur50 groups maintained the good cartilage cell membrane intact. Compared with 
      control group, the expressions of MMP-2, MCP-1, L-selectin and AOPP in OA group, 
      Cur25 group and Cur50 group were increased (all P<0.05), while CD47 levels were 
      decreased (all P<0.05). Compared with OA group, the expressions of MMP-2, MCP-1, 
      L-selectin and AOPP in Cur25 group and Cur50 group were decreased (all P<0.05), 
      while CD47 levels were increased (all P<0.05), and such changes were more 
      significant in Cur50 group (all P<0.05). Conclusion: The MMP-2, MCP-1, CD47, 
      L-selectin and AOPP are closely associated with the pathology course of OA. 
      Curcumin has protection effect on cartilage, which can relieve joint cartilage 
      degeneration, reduce cartilage inflammation and increase the metabolic activity 
      of chondrocytes.
FAU - Liu, Jun
AU  - Liu J
AD  - Department of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 
      730050, China.
FAU - He, Xiaole
AU  - He X
AD  - Department of Gerontology, Xijing Hospital, the Fourth Military Medical 
      University, Xi'an 710032, China.
FAU - Zhen, Ping
AU  - Zhen P
AD  - Department of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 
      730050, China.
FAU - Zhou, Shenghu
AU  - Zhou S
AD  - Department of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 
      730050, China.
FAU - Li, Xusheng
AU  - Li X
AD  - Department of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 
      730050, China. lixush1968@sina.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhejiang Da Xue Xue Bao Yi Xue Ban
JT  - Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical 
      sciences
JID - 100927946
RN  - 0 (Advanced Oxidation Protein Products)
RN  - 0 (Biomarkers)
RN  - 0 (CD47 Antigen)
RN  - 0 (Cd47 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 126880-86-2 (L-Selectin)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Advanced Oxidation Protein Products/metabolism
MH  - Animals
MH  - Biomarkers
MH  - CD47 Antigen/metabolism
MH  - Cartilage/chemistry/drug effects/*pathology
MH  - Chemokine CCL2/metabolism
MH  - Chondrocytes/drug effects/*pathology
MH  - Curcumin/administration & dosage/*pharmacology
MH  - Cytokines
MH  - L-Selectin/metabolism
MH  - Male
MH  - Matrix Metalloproteinase 2/metabolism
MH  - Mice, Inbred C57BL
MH  - Osteoarthritis/genetics/*pathology/physiopathology
MH  - Oxidative Stress
PMC - PMC10396913
EDAT- 2017/01/15 06:00
MHDA- 2017/09/28 06:00
PMCR- 2016/09/25
CRDT- 2017/01/15 06:00
PHST- 2017/01/15 06:00 [entrez]
PHST- 2017/01/15 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
PHST- 2016/09/25 00:00 [pmc-release]
AID - zjdxxbyxb-45-5-461 [pii]
AID - 10.3785/j.issn.1008-9292.2016.09.03 [doi]
PST - ppublish
SO  - Zhejiang Da Xue Xue Bao Yi Xue Ban. 2016 May 25;45(5):461-468. doi: 
      10.3785/j.issn.1008-9292.2016.09.03.