PMID- 2808832
OWN - NLM
STAT- MEDLINE
DCOM- 19891207
LR  - 20190709
IS  - 0190-9622 (Print)
IS  - 0190-9622 (Linking)
VI  - 21
IP  - 5 Pt 1
DP  - 1989 Nov
TI  - Reproduction of the characteristic morphologic changes of familial benign chronic 
      pemphigus in cultures of lesional keratinocytes onto dead deepidermized dermis.
PG  - 961-5
AB  - We report the in vitro reproduction of the classic histologic and ultrastructural 
      features of familial benign chronic pemphigus (FBCP) by seeding suspensions of 
      lesional keratinocytes onto healthy heterologous dead deepidermized dermis (DED). 
      With the use of normal keratinocytes, control cultures showed a 
      well-differentiated epidermis, with keratohyaline granules and lamellar bodies. 
      To the best of our knowledge this is the first successful culture of FBCP with 
      the use of dispersed lesional keratinocytes. The results suggest that in FBCP the 
      epidermis is the site of the defect leading to acantholysis, without any dermal 
      contribution.
FAU - De Dobbeleer, G
AU  - De Dobbeleer G
AD  - Department of Dermatology, Hopital Erasme, Universite Libre de Bruxelles, 
      Belgium.
FAU - De Graef, C
AU  - De Graef C
FAU - M'Poudi, E
AU  - M'Poudi E
FAU - Gourdain, J M
AU  - Gourdain JM
FAU - Heenen, M
AU  - Heenen M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Am Acad Dermatol
JT  - Journal of the American Academy of Dermatology
JID - 7907132
RN  - 0 (Culture Media)
SB  - IM
MH  - Cell Division
MH  - Cells, Cultured
MH  - Chronic Disease
MH  - Culture Media
MH  - Epithelium/physiology
MH  - Humans
MH  - Keratinocytes/*physiology/ultrastructure
MH  - Pemphigus/*pathology
MH  - Skin Physiological Phenomena
EDAT- 1989/11/01 00:00
MHDA- 1989/11/01 00:01
CRDT- 1989/11/01 00:00
PHST- 1989/11/01 00:00 [pubmed]
PHST- 1989/11/01 00:01 [medline]
PHST- 1989/11/01 00:00 [entrez]
AID - S0190-9622(89)70283-8 [pii]
AID - 10.1016/s0190-9622(89)70283-8 [doi]
PST - ppublish
SO  - J Am Acad Dermatol. 1989 Nov;21(5 Pt 1):961-5. doi: 
      10.1016/s0190-9622(89)70283-8.