PMID- 28090559
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220915
IS  - 2332-8940 (Print)
IS  - 2332-8959 (Electronic)
IS  - 2332-8940 (Linking)
VI  - 3
IP  - 4
DP  - 2016
TI  - Potentiation of Ecstasy-induced hyperthermia and FAT/CD36 expression in 
      chronically exercised animals.
PG  - 557-566
LID - 10.1080/23328940.2016.1166310 [doi]
AB  - Fatal hyperthermia as a result of 3,4-methylenedioxymethamphetamine (MDMA) use 
      involves non-esterified free fatty acids (NEFA) and the activation of 
      mitochondrial uncoupling proteins (UCP). NEFA gain access into skeletal muscle 
      via specific transport proteins, including fatty acid translocase (FAT/CD36). 
      FAT/CD36 expression is known to increase following chronic exercise. Previous 
      studies have demonstrated the essential role of NEFA and UCP3 in MDMA-induced 
      hyperthermia. The aims of the present study were to use a chronic exercise model 
      (swimming for two consecutive hours per day, five days per wk for six wk) to 
      increase FAT/CD36 expression in order to: 1) determine the contribution of 
      FAT/CD36 in MDMA (20 mg/kg, s.c.)-mediated hyperthermia; and 2) examine the 
      effects of the FAT/CD36 inhibitor, SSO (sulfo-N-succinimidyl oleate), on 
      MDMA-induced hyperthermia in chronic exercise and sedentary control rats. MDMA 
      administration resulted in hyperthermia in both sedentary and chronic exercise 
      animals. However, MDMA-induced hyperthermia was significantly potentiated in the 
      chronic exercise animals compared to sedentary animals. Additionally, chronic 
      exercise significantly reduced body weight, increased FAT/CD36 protein expression 
      levels and reduced plasma NEFA levels. The FAT/CD36 inhibitor, SSO (40 mg/kg, 
      ip), significantly attenuated the hyperthermia mediated by MDMA in chronic 
      exercised but not sedentary animals. Plasma NEFA levels were elevated in 
      sedentary and exercised animals treated with SSO prior to MDMA suggesting 
      attenuation of NEFA uptake into skeletal muscle. Chronic exercise did not alter 
      skeletal muscle UCP3 protein expression levels. In conclusion, chronic exercise 
      potentiates MDMA-mediated hyperthermia in a FAT/CD36 dependent fashion.
FAU - Hrometz, Sandra L
AU  - Hrometz SL
AD  - Department of Pharmaceutical Sciences, College of Pharmacy, Natural and Health 
      Sciences, Manchester University , Fort Wayne, IN, USA.
FAU - Ebert, Jeremy A
AU  - Ebert JA
AD  - Department of Pharmaceutical and Biomedical Sciences, The Raabe College of 
      Pharmacy, Ohio Northern University , Ada, OH, USA.
FAU - Grice, Karen E
AU  - Grice KE
AD  - Department of Pharmaceutical and Biomedical Sciences, The Raabe College of 
      Pharmacy, Ohio Northern University , Ada, OH, USA.
FAU - Nowinski, Sara M
AU  - Nowinski SM
AD  - Division of Pharmacology and Toxicology, College of Pharmacy, University of Texas 
      at Austin , Austin, TX, USA.
FAU - Mills, Edward M
AU  - Mills EM
AD  - Division of Pharmacology and Toxicology, College of Pharmacy, University of Texas 
      at Austin , Austin, TX, USA.
FAU - Myers, Brian J
AU  - Myers BJ
AD  - Department of Chemistry and Biochemistry, The Getty College of Arts & Sciences, 
      Ohio Northern University ; Ada, OH, USA.
FAU - Sprague, Jon E
AU  - Sprague JE
AD  - The Ohio Attorney General's Center for the Future of Forensic Science, Bowling 
      Green State University , Bowling Green, OH, USA.
LA  - eng
GR  - R01 DK089224/DK/NIDDK NIH HHS/United States
GR  - R56 DK089224/DK/NIDDK NIH HHS/United States
PT  - Journal Article
DEP - 20160330
PL  - United States
TA  - Temperature (Austin)
JT  - Temperature (Austin, Tex.)
JID - 101655855
PMC - PMC5198810
OTO - NOTNLM
OT  - FAT/CD36
OT  - MDMA
OT  - chronic exercise
OT  - hyperthermia
OT  - sulfo-N-succinimidyl oleate
EDAT- 2017/01/17 06:00
MHDA- 2017/01/17 06:01
PMCR- 2016/03/30
CRDT- 2017/01/17 06:00
PHST- 2016/02/26 00:00 [received]
PHST- 2016/03/10 00:00 [revised]
PHST- 2016/03/10 00:00 [accepted]
PHST- 2017/01/17 06:00 [entrez]
PHST- 2017/01/17 06:00 [pubmed]
PHST- 2017/01/17 06:01 [medline]
PHST- 2016/03/30 00:00 [pmc-release]
AID - 1166310 [pii]
AID - 10.1080/23328940.2016.1166310 [doi]
PST - epublish
SO  - Temperature (Austin). 2016 Mar 30;3(4):557-566. doi: 
      10.1080/23328940.2016.1166310. eCollection 2016.