PMID- 28091547
OWN - NLM
STAT- MEDLINE
DCOM- 20181015
LR  - 20181217
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
DP  - 2017 Jan 16
TI  - Corydalis edulis Maxim. Promotes Insulin Secretion via the Activation of Protein 
      Kinase Cs (PKCs) in Mice and Pancreatic beta Cells.
PG  - 40454
LID - 10.1038/srep40454 [doi]
LID - 40454
AB  - Corydalis edulis Maxim., a widely grown plant in China, had been proposed for the 
      treatment for type 2 diabetes mellitus. In this study, we found that C. edulis 
      extract (CE) is protective against diabetes in mice. The treatment of 
      hyperglycemic and hyperlipidemic apolipoprotein E (ApoE)-/- mice with a high dose 
      of CE reduced serum glucose by 28.84% and serum total cholesterol by 17.34% and 
      increased insulin release. We also found that CE significantly enhanced insulin 
      secretion in a glucose-independent manner in hamster pancreatic beta cell (HIT-T15). 
      Further investigation revealed that CE stimulated insulin exocytosis by a protein 
      kinase C (PKC)-dependent signaling pathway and that CE selectively activated 
      novel protein kinase Cs (nPKCs) and atypical PKCs (aPKCs) but not conventional 
      PKCs (cPKCs) in HIT-T15 cells. To the best of our knowledge, our study is the 
      first to identify the PKC pathway as a direct target and one of the major 
      mechanisms underlying the antidiabetic effect of CE. Given the good 
      insulinotropic effect of this herbal medicine, CE is a promising agent for the 
      development of new drugs for treating diabetes.
FAU - Zheng, Jiao
AU  - Zheng J
AD  - Modern Research Center for Traditional Chinese Medicine, Beijing University of 
      Chinese Medicine, Beijing 100029, China.
FAU - Zhao, Yunfang
AU  - Zhao Y
AD  - Modern Research Center for Traditional Chinese Medicine, Beijing University of 
      Chinese Medicine, Beijing 100029, China.
FAU - Lun, Qixing
AU  - Lun Q
AD  - Modern Research Center for Traditional Chinese Medicine, Beijing University of 
      Chinese Medicine, Beijing 100029, China.
AD  - School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 
      100102, China.
FAU - Song, Yuelin
AU  - Song Y
AD  - Modern Research Center for Traditional Chinese Medicine, Beijing University of 
      Chinese Medicine, Beijing 100029, China.
FAU - Shi, Shepo
AU  - Shi S
AD  - Modern Research Center for Traditional Chinese Medicine, Beijing University of 
      Chinese Medicine, Beijing 100029, China.
FAU - Gu, Xiaopan
AU  - Gu X
AD  - Modern Research Center for Traditional Chinese Medicine, Beijing University of 
      Chinese Medicine, Beijing 100029, China.
AD  - School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 
      100102, China.
FAU - Pan, Bo
AU  - Pan B
AD  - Modern Research Center for Traditional Chinese Medicine, Beijing University of 
      Chinese Medicine, Beijing 100029, China.
AD  - School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 
      100102, China.
FAU - Qu, Changhai
AU  - Qu C
AD  - Modern Research Center for Traditional Chinese Medicine, Beijing University of 
      Chinese Medicine, Beijing 100029, China.
FAU - Li, Jun
AU  - Li J
AD  - Modern Research Center for Traditional Chinese Medicine, Beijing University of 
      Chinese Medicine, Beijing 100029, China.
FAU - Tu, Pengfei
AU  - Tu P
AD  - Modern Research Center for Traditional Chinese Medicine, Beijing University of 
      Chinese Medicine, Beijing 100029, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170116
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Apolipoproteins E)
RN  - 0 (Blood Glucose)
RN  - 0 (Insulin)
RN  - 0 (Plant Extracts)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Triglycerides)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Animals
MH  - Apolipoproteins E/deficiency/metabolism
MH  - Blood Glucose/metabolism
MH  - Cell Membrane/drug effects/metabolism
MH  - Cholesterol/metabolism
MH  - Corydalis/*chemistry
MH  - Cricetinae
MH  - Cytosol/drug effects/metabolism
MH  - Enzyme Activation/drug effects
MH  - Exocytosis/drug effects
MH  - Fasting/blood
MH  - Glucose Tolerance Test
MH  - Insulin/blood/*metabolism
MH  - Insulin Secretion
MH  - Insulin-Secreting Cells/drug effects/*enzymology
MH  - Male
MH  - Mice
MH  - Plant Extracts/*pharmacology
MH  - Protein Kinase C/*metabolism
MH  - Protein Transport/drug effects
MH  - RNA, Small Interfering/metabolism
MH  - Transfection
MH  - Triglycerides/metabolism
PMC - PMC5238372
EDAT- 2017/01/17 06:00
MHDA- 2018/10/16 06:00
PMCR- 2017/01/16
CRDT- 2017/01/17 06:00
PHST- 2016/08/16 00:00 [received]
PHST- 2016/11/30 00:00 [accepted]
PHST- 2017/01/17 06:00 [entrez]
PHST- 2017/01/17 06:00 [pubmed]
PHST- 2018/10/16 06:00 [medline]
PHST- 2017/01/16 00:00 [pmc-release]
AID - srep40454 [pii]
AID - 10.1038/srep40454 [doi]
PST - epublish
SO  - Sci Rep. 2017 Jan 16;7:40454. doi: 10.1038/srep40454.