PMID- 28092843
OWN - NLM
STAT- MEDLINE
DCOM- 20170327
LR  - 20170327
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 88
DP  - 2017 Apr
TI  - Therapeutic effects of bach1 siRNA on human breast adenocarcinoma cell line.
PG  - 34-42
LID - S0753-3322(16)31634-1 [pii]
LID - 10.1016/j.biopha.2017.01.030 [doi]
AB  - BACKGROUND: Despite the great improvements in clinical and therapeutic techniques 
      in recent years, many advanced breast cancer patients still died of the 
      postoperative recurrence and metastasis of disease. Bach1 plays a role in the 
      development of the invasive phenotypes of cancer, cell division and apoptosis in 
      tumor cells. The aim of this study was to investigate the effect of specific 
      bach1 siRNAs, on the proliferation, migration, invasive, induction of apoptosis, 
      cell cycle arrest and alter EMT related miRNA of MDA-MB-468 cells (breast 
      cancer). METHODS: siRNA transfection was performed with transfection reagent. The 
      expression levels of Bach1 mRNA and protein were measured by qRT-PCR and western 
      blot analysis, respectively. The survival of cells was determined using MTT assay 
      cells, apoptosis using Tunel assay, Cell migration using scratch assay and Cell 
      cycle analysis by Propidium Iodide (PI) DNA staining method by using flow 
      cytometry on the MDA-MB-468. The expression levels of MMP-9 and CXCR4 were 
      measured by qRT-PCR. RESULTS: Transfection with siRNA significantly suppressed 
      the expression of bach1 gene in dose dependent manner after 48h (p<0.0001). 
      Surprisingly, treatment with bach1 siRNA arrest cell cycle in S phases 
      (p<0.0001). Moreover siRNA transfection had effects on breast adenocarcinoma 
      cells and inhibits the migration (p<0.0001), proliferation (p<0.0001), cell cycle 
      arrest (p=0.03) and induces apoptosis (p<0.0001) and reduces the expression of 
      miR-21 (P=0.0014). CONCLUSION: Our results suggest that the bach1 can be 
      considered as a potent adjuvant in breast cancer therapy.
CI  - Copyright (c) 2017 Elsevier Masson SAS. All rights reserved.
FAU - Aletaha, Mansoor
AU  - Aletaha M
AD  - Biotechnology, Department of Pathobiology, Shiraz University, Shiraz, Iran.
FAU - Mansoori, Behzad
AU  - Mansoori B
AD  - Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
FAU - Mohammadi, Ali
AU  - Mohammadi A
AD  - Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
FAU - Fazeli, Mehdi
AU  - Fazeli M
AD  - Department of Pharmacology and Toxicology, School of Veterinary Medicine, Shiraz 
      University, Shiraz, Iran.
FAU - Baradaran, Behzad
AU  - Baradaran B
AD  - Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. 
      Electronic address: behzad_im@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20170113
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (BACH1 protein, human)
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (CXCR4 protein, human)
RN  - 0 (Fanconi Anemia Complementation Group Proteins)
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, CXCR4)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Adenocarcinoma/*genetics/pathology
MH  - Apoptosis/genetics
MH  - Basic-Leucine Zipper Transcription Factors/genetics/*metabolism
MH  - Breast Neoplasms/*genetics/pathology
MH  - Cell Cycle Checkpoints/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation
MH  - Fanconi Anemia Complementation Group Proteins/genetics/*metabolism
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - Gene Silencing
MH  - Humans
MH  - Matrix Metalloproteinase 9/genetics/metabolism
MH  - MicroRNAs/genetics/metabolism
MH  - Neoplasm Metastasis
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Small Interfering/*therapeutic use
MH  - Receptors, CXCR4/genetics/metabolism
MH  - Transfection
OTO - NOTNLM
OT  - Bach1
OT  - Breast cancer
OT  - MDA-MB-468
OT  - RNA interference
OT  - siRNA
EDAT- 2017/01/17 06:00
MHDA- 2017/03/28 06:00
CRDT- 2017/01/17 06:00
PHST- 2016/09/17 00:00 [received]
PHST- 2017/01/05 00:00 [accepted]
PHST- 2017/01/17 06:00 [pubmed]
PHST- 2017/03/28 06:00 [medline]
PHST- 2017/01/17 06:00 [entrez]
AID - S0753-3322(16)31634-1 [pii]
AID - 10.1016/j.biopha.2017.01.030 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2017 Apr;88:34-42. doi: 10.1016/j.biopha.2017.01.030. Epub 
      2017 Jan 13.