PMID- 28095443
OWN - NLM
STAT- MEDLINE
DCOM- 20170822
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 1
DP  - 2017
TI  - Morphologic and Molecular Characterization of a Strain of Zika Virus Imported 
      into Guangdong, China.
PG  - e0169256
LID - 10.1371/journal.pone.0169256 [doi]
LID - e0169256
AB  - The recent outbreaks of Zika virus (ZIKV) disease have caused worldwide concerns. 
      Guangdong province is one of the commercial centers in China and communicates 
      frequently with the epidemic areas. To date, 65.2% of the ZIKV infection cases in 
      China were imported via port of entry in Guangdong. The continuous surveillance 
      of imported cases is crucial for the prevention and control of potential ZIKV 
      infection outbreak in China. In this study, a strain of ZIKV was isolated from 
      the serum of a 6-year-old child returning from Venezuela. The morphology of the 
      ZIKV was analyzed in vivo and in vitro by electron microscopy, and clusters of 
      virus particles were found in the loose cytoplasmic membrane structures. The 
      genomic sequence of the isolated ZIKV was determined, and the alignment and 
      phylogenetic analysis identified one unique amino acid substitution occurring in 
      the non-structural protein 4B (NS4B), and the isolated virus belonged to the 
      Asian lineage.
FAU - Li, Shufen
AU  - Li S
AD  - Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and 
      Quarantine Bureau, Guangzhou, P. R. China.
AD  - State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of 
      Guangzhou Medical University, Guangzhou, P. R. China.
FAU - Shi, Yongxia
AU  - Shi Y
AD  - Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and 
      Quarantine Bureau, Guangzhou, P. R. China.
FAU - Zheng, Kui
AU  - Zheng K
AD  - Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and 
      Quarantine Bureau, Guangzhou, P. R. China.
FAU - Dai, Jun
AU  - Dai J
AD  - Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and 
      Quarantine Bureau, Guangzhou, P. R. China.
FAU - Li, Xiaobo
AU  - Li X
AD  - Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and 
      Quarantine Bureau, Guangzhou, P. R. China.
FAU - Yuan, Shuai
AU  - Yuan S
AD  - Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and 
      Quarantine Bureau, Guangzhou, P. R. China.
FAU - Chen, Ling
AU  - Chen L
AD  - State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of 
      Guangzhou Medical University, Guangzhou, P. R. China.
FAU - Huang, Jicheng
AU  - Huang J
AD  - Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and 
      Quarantine Bureau, Guangzhou, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20170117
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Child
MH  - Genome, Viral
MH  - Humans
MH  - Mice
MH  - Microscopy, Electron
MH  - Phylogeny
MH  - RNA, Viral/genetics
MH  - Saliva/virology
MH  - Sequence Homology, Amino Acid
MH  - Urine/virology
MH  - Venezuela/epidemiology
MH  - Viral Load
MH  - Zika Virus/genetics/*isolation & purification/*ultrastructure
MH  - Zika Virus Infection/*diagnosis/epidemiology/*transmission
PMC - PMC5240961
COIS- The authors have declared that no competing interests exist.
EDAT- 2017/01/18 06:00
MHDA- 2017/08/23 06:00
PMCR- 2017/01/17
CRDT- 2017/01/18 06:00
PHST- 2016/08/11 00:00 [received]
PHST- 2016/12/14 00:00 [accepted]
PHST- 2017/01/18 06:00 [entrez]
PHST- 2017/01/18 06:00 [pubmed]
PHST- 2017/08/23 06:00 [medline]
PHST- 2017/01/17 00:00 [pmc-release]
AID - PONE-D-16-31216 [pii]
AID - 10.1371/journal.pone.0169256 [doi]
PST - epublish
SO  - PLoS One. 2017 Jan 17;12(1):e0169256. doi: 10.1371/journal.pone.0169256. 
      eCollection 2017.