PMID- 28095444
OWN - NLM
STAT- MEDLINE
DCOM- 20170818
LR  - 20240327
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 1
DP  - 2017
TI  - The Role of DCT in HPV16 Infection of HaCaTs.
PG  - e0170158
LID - 10.1371/journal.pone.0170158 [doi]
LID - e0170158
AB  - Persistent infection with high-risk human papillomavirus (HPV) genotype is a 
      major factor leading to many human cancers. Mechanisms of HPV entry into host 
      cells and genome trafficking towards the nucleus are incompletely understood. 
      Dopachrome tautomerase (DCT) was identified as a cellular gene required for HPV 
      infection in HeLa cells on a siRNA screen study. Here, we confirm that DCT 
      knockdown significantly decreases HPV infection in the human keratinocyte HaCaT 
      cells as was observed in HeLas. We investigated the effects of DCT knockdown and 
      found that DCT depletion caused increased reactive oxygen species (ROS) levels, 
      DNA damage and altered cell cycle in HaCaT cells. We observed increased viral DNA 
      localization at the endoplasmic reticulum but an overall decrease in infection in 
      DCT knockdown cells. This observation suggests that viral DNA might be retained 
      in the ER due to altered cell cycle, and viral particles are incapable of further 
      movement towards the nucleus in DCT knockdown cells.
FAU - Aksoy, Pinar
AU  - Aksoy P
AD  - Department of Biological Sciences, Fordham University, Bronx, New York, United 
      States of America.
FAU - Meneses, Patricio I
AU  - Meneses PI
AD  - Department of Biological Sciences, Fordham University, Bronx, New York, United 
      States of America.
LA  - eng
GR  - R21 CA153096/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20170117
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 3.4.- (Amyloid Precursor Protein Secretases)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.3.12 (dopachrome isomerase)
SB  - IM
MH  - Amyloid Precursor Protein Secretases/metabolism
MH  - Cells, Cultured
MH  - DNA, Viral/genetics
MH  - Endoplasmic Reticulum/enzymology/virology
MH  - Human papillomavirus 16/*enzymology
MH  - Humans
MH  - Intramolecular Oxidoreductases/*antagonists & inhibitors/genetics/metabolism
MH  - Keratinocytes/enzymology/pathology/*virology
MH  - Papillomavirus Infections/genetics/metabolism/*virology
MH  - RNA, Small Interfering/*genetics
MH  - Reactive Oxygen Species/metabolism
PMC - PMC5240977
COIS- The authors have declared that no competing interests exist.
EDAT- 2017/01/18 06:00
MHDA- 2017/08/19 06:00
PMCR- 2017/01/17
CRDT- 2017/01/18 06:00
PHST- 2016/10/08 00:00 [received]
PHST- 2016/12/30 00:00 [accepted]
PHST- 2017/01/18 06:00 [entrez]
PHST- 2017/01/18 06:00 [pubmed]
PHST- 2017/08/19 06:00 [medline]
PHST- 2017/01/17 00:00 [pmc-release]
AID - PONE-D-16-40131 [pii]
AID - 10.1371/journal.pone.0170158 [doi]
PST - epublish
SO  - PLoS One. 2017 Jan 17;12(1):e0170158. doi: 10.1371/journal.pone.0170158. 
      eCollection 2017.