PMID- 28097166
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2322-181X (Print)
IS  - 2345-2005 (Electronic)
IS  - 2322-181X (Linking)
VI  - 5
IP  - 3
DP  - 2016 Sep
TI  - Molecular characterization of Pasteurella multocida isolates obtained from 
      poultry, ruminant, cats and dogs using RAPD and REP-PCR analysis.
PG  - 123-132
AB  - In the present study, Randomly Amplified Polymorphic DNA (RAPD) and Repetitive 
      Extragenic Palindromic sequence-based Polymerase Chain Reaction (REP- PCR) were 
      used to characterize 131 isolates of Pasteurella multocida, originating from 
      different healthy and diseased animal species obtained from several geographical 
      regions of Iran. The RAPD and REP-PCR generated amplified products in the range 
      of 300 to 3400 bp and 200 to 2850 bp, respectively. Among all of the P. multocida 
      isolates, cluster analysis revealed that 63 clusters and nine untypable isolates 
      and 81 clusters and six untypable isolates were produced with RAPD and REP-PCR 
      methods, respectively. The results indicated that the REP-PCR method showed a 
      slightly higher level of discrimination power in differentiating of P. multocida 
      isolates as compared with RAPD. The results showed that a considerable level of 
      genetic diversity exists among P. multocida isolates even in the isolates with 
      the same animal or geographical origins. There was no host- and region-specific 
      pattern. In addition, the isolates obtained from the healthy and diseased animal 
      did not reveal any correlation genotypic profiles, which could be supported by 
      the hypothesis that P. multocida is a strictly opportunistic pathogen. In 
      conclusion, because of a large amount of genetic heterogeneity in the P. 
      multocida isolates, Pasteurellosis may be caused by different clones in the same 
      herd or animal.
FAU - Shirzad-Aski, Hesamaddin
AU  - Shirzad-Aski H
AD  - Department of Pathobiology, School of Veterinary Medicine, Shiraz University, 
      Shiraz, Iran.
FAU - Tabatabaei, Mohammad
AU  - Tabatabaei M
AD  - Department of Pathobiology, School of Veterinary Medicine, Shiraz University, 
      Shiraz, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Mol Biol Res Commun
JT  - Molecular biology research communications
JID - 101613459
PMC - PMC5219907
OTO - NOTNLM
OT  - Molecular analysis
OT  - Pasteurella multocida
OT  - RAPD
OT  - REP-PCR
EDAT- 2017/01/18 06:00
MHDA- 2017/01/18 06:01
PMCR- 2016/09/01
CRDT- 2017/01/19 06:00
PHST- 2017/01/19 06:00 [entrez]
PHST- 2017/01/18 06:00 [pubmed]
PHST- 2017/01/18 06:01 [medline]
PHST- 2016/09/01 00:00 [pmc-release]
AID - mbrc-5-123 [pii]
PST - ppublish
SO  - Mol Biol Res Commun. 2016 Sep;5(3):123-132.