PMID- 28097463
OWN - NLM
STAT- MEDLINE
DCOM- 20170307
LR  - 20210109
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 42
IP  - 2
DP  - 2017 Feb
TI  - Neurokinin-1 Receptor-Immunopositive Neurons in the Medullary Dorsal Horn Provide 
      Collateral Axons to both the Thalamus and Parabrachial Nucleus in Rats.
PG  - 375-388
LID - 10.1007/s11064-016-2080-0 [doi]
AB  - It has been suggested that the trigemino-thalamic and trigemino-parabrachial 
      projection neurons in the medullary dorsal horn (MDH) are highly implicated in 
      the sensory-discriminative and emotional/affective aspects of orofacial pain, 
      respectively. In previous studies, some neurons were reported to send projections 
      to both the thalamus and parabrachial nucleus by way of collaterals in the MDH. 
      However, little is known about the chemoarchitecture of this group of neurons. 
      Thus, in the present study, we determined whether the neurokinin-1 (NK-1) 
      receptor, which is crucial for primary orofacial pain signaling, was expressed in 
      MDH neurons co-innervating the thalamus and parabrachial nucleus. Vesicular 
      glutamate transporter 2 (VGLUT2) mRNA, a biomarker for the subgroup of 
      glutamatergic neurons closely related to pain sensation, was assessed in 
      trigemino-parabrachial projection neurons in the MDH. After stereotactic 
      injection of fluorogold (FG) and cholera toxin subunit B (CTB) into the ventral 
      posteromedial thalamic nucleus (VPM) and parabrachial nucleus (PBN), 
      respectively, triple labeling with fluorescence dyes for FG, CTB and NK-1 
      receptor (NK-1R) revealed that approximately 76 % of the total FG/CTB dually 
      labeled neurons were detected as NK-1R-immunopositive, and more than 94 % of the 
      triple-labeled neurons were distributed in lamina I. In addition, by FG 
      retrograde tract-tracing combined with fluorescence in situ hybridization (FISH) 
      for VGLUT2 mRNA, 54, 48 and 70 % of FG-labeled neurons in laminae I, II and III, 
      respectively, of the MDH co-expressed FG and VGLUT2 mRNA. Thus, most of the MDH 
      neurons co-innervating the thalamus and PBN were glutamatergic. Most MDH neurons 
      providing the collateral axons to both the thalamus and parabrachial nucleus in 
      rats were NK-1R-immunopositive and expressed VGLUT2 mRNA. NK-1R and VGLUT2 in MDH 
      neurons may be involved in both sensory-discriminative and emotional/affective 
      aspects of orofacial pain processing.
FAU - Li, Xu
AU  - Li X
AD  - Department of Colorectal Surgery, Changhai Hospital, The Second Military Medical 
      University, Shanghai, 200433, China.
FAU - Ge, Shun-Nan
AU  - Ge SN
AD  - Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical 
      University, Xi'an, 710038, China. gesn8561@fmmu.edu.cn.
FAU - Li, Yang
AU  - Li Y
AD  - Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical 
      University, Xi'an, 710038, China.
FAU - Wang, Han-Tao
AU  - Wang HT
AD  - Department of Colorectal Surgery, Changhai Hospital, The Second Military Medical 
      University, Shanghai, 200433, China. hantaowang@126.com.
LA  - eng
PT  - Journal Article
DEP - 20170117
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Receptors, Neurokinin-1)
SB  - IM
MH  - Animals
MH  - Axons/*chemistry/metabolism
MH  - Male
MH  - Medulla Oblongata/*chemistry/metabolism
MH  - Parabrachial Nucleus/*chemistry/metabolism
MH  - Posterior Horn Cells/*chemistry/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Neurokinin-1/*analysis/metabolism
MH  - Thalamus/*chemistry/metabolism
OTO - NOTNLM
OT  - Medullary dorsal horn
OT  - Neurokinin-1 receptor
OT  - Parabrachial nucleus
OT  - Rat
OT  - Ventral posteromedial thalamic nucleus
OT  - Vesicular glutamate transporters
EDAT- 2017/01/18 06:00
MHDA- 2017/03/08 06:00
CRDT- 2017/01/19 06:00
PHST- 2016/03/17 00:00 [received]
PHST- 2016/10/04 00:00 [accepted]
PHST- 2016/08/18 00:00 [revised]
PHST- 2017/01/18 06:00 [pubmed]
PHST- 2017/03/08 06:00 [medline]
PHST- 2017/01/19 06:00 [entrez]
AID - 10.1007/s11064-016-2080-0 [pii]
AID - 10.1007/s11064-016-2080-0 [doi]
PST - ppublish
SO  - Neurochem Res. 2017 Feb;42(2):375-388. doi: 10.1007/s11064-016-2080-0. Epub 2017 
      Jan 17.