PMID- 28098926
OWN - NLM
STAT- MEDLINE
DCOM- 20170929
LR  - 20190116
IS  - 1473-2262 (Electronic)
IS  - 1473-2262 (Linking)
VI  - 33
DP  - 2017 Jan 18
TI  - Relevance of osteoclast-specific enzyme activities in cell-based in vitro 
      resorption assays.
PG  - 28-42
LID - 10.22203/eCM.v033a03 [doi]
AB  - Cell-based in vitro resorption assays are an important tool to simulate the in 
      vivo biodegradation of resorbable bone graft materials and to predict their 
      clinical performance. The present study analyses the activity of 
      osteoclast-specific enzymes as potential surrogate measures for classical pit 
      assay, which is not applicable on irregular structured materials. Osteoclasts 
      derived from human peripheral blood mononuclear cells were cultivated on 
      different surfaces: calcium phosphate bone cements (CPC), dentin discs, 
      osteoblast-derived extracellular matrix (ECM) and tissue culture polystyrene as 
      control. Pit formation on the resorbable materials was investigated and 
      correlated with the activity of tartrate resistant acid phosphatase (TRAP), 
      carbonic anhydrase II (CAII) and cathepsin K (CTSK). Furthermore, the relation 
      between intra- and extracellular enzyme activities was examined for TRAP and CTSK 
      during resorption of the different materials. Resorbed area of CPC correlated 
      with intracellular TRAP activity and intracellular CAII activity. Highest 
      resorption was detected at around pH 7.2. Resorbed area on dentin correlated with 
      the extracellular CTSK activity and extracellular TRAP activity and was maximal 
      at around pH 6.8. Osteoclasts cultivated on cell-derived mineralised ECM showed a 
      good correlation between both extracellular TRAP and CTSK activity and the 
      release of calcium ions. Based on these data a different regulation of TRAP and 
      CTSK secretion is hypothesised for the resorption of inorganic calcium phosphate 
      compared to the resorption of collagenous mineralised matrix.
FAU - Bernhardt, A
AU  - Bernhardt A
AD  - Fetscherstr. 42, 01309 Dresden, Germany.anne.bernhardt@tu-dresden.de.
FAU - Koperski, K
AU  - Koperski K
FAU - Schumacher, M
AU  - Schumacher M
FAU - Gelinsky, M
AU  - Gelinsky M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170118
PL  - United States
TA  - Eur Cell Mater
JT  - European cells & materials
JID - 100973416
RN  - 0 (Bone Cements)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Polystyrenes)
RN  - 97Z1WI3NDX (calcium phosphate)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
SB  - IM
MH  - Biological Assay/*methods
MH  - Bone Cements/pharmacology
MH  - Bone Matrix/drug effects/metabolism
MH  - Bone Resorption/*enzymology/pathology
MH  - Calcium Phosphates/pharmacology
MH  - Cell Differentiation/drug effects
MH  - Dentin/metabolism
MH  - Extracellular Matrix/drug effects/metabolism
MH  - Humans
MH  - Osteoblasts/drug effects/enzymology/pathology
MH  - Osteoclasts/drug effects/*enzymology/pathology/ultrastructure
MH  - Polystyrenes/pharmacology
MH  - Staining and Labeling
MH  - Tartrate-Resistant Acid Phosphatase/metabolism
EDAT- 2017/01/19 06:00
MHDA- 2017/09/30 06:00
CRDT- 2017/01/19 06:00
PHST- 2017/01/19 06:00 [entrez]
PHST- 2017/01/19 06:00 [pubmed]
PHST- 2017/09/30 06:00 [medline]
AID - vol033a03 [pii]
AID - 10.22203/eCM.v033a03 [doi]
PST - epublish
SO  - Eur Cell Mater. 2017 Jan 18;33:28-42. doi: 10.22203/eCM.v033a03.